... complexed with its NADP(H) cofactor, different saccharides and sugar alcohols. The structures demonstrate the molecular basis for substrate binding and allowed us to present a reaction mechanism for the enzyme. carbohydrate crystal structure enzyme mechanism glucose-fructose oxidoreductase/inositol...

... that represents a novel type of sialic acid-specific polysaccharide utilization locus. Bacteroides fragilis carbohydrate nanOU outer membrane sialic acid transport The Bacteroidetes are a phylum of Gram-negative anaerobic bacteria that often have intimate relationships with their human...

...Eneas A. Chavelas; Enrique García-Hernández Carbohydrates are crucial for living cells, playing myriads of functional roles that range from being structural or energy-storage devices to molecular labels that, through non-covalent interaction with proteins, impart exquisite selectivity in processes...

... 12 12 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 Aspergillus carbohydrate endoglucanase glycosyl hydrolase (GH) xyloglucanase xyloglucan-specific endo-β-1,4-glucanase (XEG) The activities of many microbial CAZymes (carbohydrate-active enzymes...

... To whom correspondence should be addressed (e-mail aplan@iqs.es ). 26 1 2004 18 3 2004 24 3 2004 24 3 2004 The Biochemical Society, London ©2004 2004 carbohydrate (1→3,1→4)-β- d -glucan glycosynthase polysaccharide synthesis polysaccharide structure...

... of carbohydrates to form unnatural oligosaccharide products that can attain unique crystalline forms. These findings reinforce the potential of glycosynthases as interesting and important tools for the enzymic synthesis of novel carbohydrates with specific and controlled structures. 1 To whom correspondence...

... used extensively to remove carbohydrate from glycoproteins, while leaving the protein backbone intact. Glycosylated proteins from animals, plants, fungi and bacteria have been deglycosylated with TFMS, and the most extensively studied types of carbohydrate chains in mammals, the N-linked, O-linked...

... The Biochemical Society, London ©2002 2002 carbohydrate mucosal gland stomach surface epithelium Abbreviations used: AP, alkaline phosphatase; DFP, di-isopropyl phosphorofluoridate; DTT, dithiothreitol; HRP, horseradish peroxidase; Le, Lewis antigen; PBST, PBS containing 0.05% (v/v) Tween 20...

... 2001 24 5 2001 22 6 2001 The Biochemical Society, London ©2001 2001 carbohydrate intestine Sd a /Cad antigen Abbreviations used: Gal, galactose; GalNAc, N -acetylgalactosamine; GalNAc-ol, N -acetylgalactosaminitol; GlcNAc, N -acetylglucosamine; HPAEC, high pH anion...

... of immunoglobulins is recognizing a carbohydrate residue that is attached to the protein when it is synthesized by the mouse myeloma cell line, NSO, in which it is made. If the cytokine receptors are similarly glycosylated in vivo , then the presence of these antibodies in normal human serum may modulate...

...-exchange chromatography and fluorophore-assisted carbohydrate electrophoresis. The predominant components were disialyl- bi-antennary and tetra-sialyl tetra-antennary complex carbohydrates. Of note is the presence in this human IgG of oligosaccharides containing N- glycolylneuraminic acid and N...

... transcription by carbohydrates such as glucose and fructose, although the L-III element is itself responsive to carbohydrates. The L-II element is also responsible for the gene regulation by polyunsaturated fatty acids. Nuclear factor-1 proteins and hepatocyte nuclear factor 4, which bind to the L-II element...