... 4 2006 The Biochemical Society, London 2006 affinity chromatography constitutive active/androstane receptor (CAR) cytochrome P450 (CYP) phenobarbital-responsive enhancer module structural maintenance of chromosomes 1 (SMC1) xenobiotic Preparation of total RNA...

... investigated using the immune-quantification assays. Only LAMP-1 (+Tail) was found to aggregate and interact with plasma proteins. Plasma proteins that interact with LAMP-1 were isolated by affinity chromatography with either the recombinant LAMP-1 (−Tail) or a synthesized peptide consisting of the 14 amino...

... correspondence should be addressed (e-mail roderic.eckenhoff@uphs.upenn.edu ). 29 10 2003 21 1 2004 3 2 2004 3 2 2004 The Biochemical Society, London ©2004 2004 affinity chromatography binding force human serum albumin (HSA) isothermal titration calorimetry (ITC) propofol...

...Mercedes POZUELO RUBIO; Kathryn M. GERAGHTY; Barry H. C. WONG; Nicola T. WOOD; David G. CAMPBELL; Nick MORRICE; Carol MACKINTOSH 14-3-3-interacting proteins were isolated from extracts of proliferating HeLa cells using 14-3-3 affinity chromatography, eluting with a phosphopeptide that competes...

... that bind to phosphoserine/phosphothreonine-containing sequences are the 14-3-3 proteins, which regulate a wide range of cellular targets. A new analysis of the 14-3-3 phosphoproteome using affinity chromatography has revealed many previously unknown 14-3-3 ligands whose binding to 14-3-3 proteins...

...Dipak PATEL; Srdjan VITOVSKI; H. Jennifer SENIOR; Michael D. EDGE; Robert C. HOCKNEY; Michael J. DEMPSEY; Jon R. SAYERS A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique process that mimics...

... for identifying and analysing the cellular enzyme/protein–ubiquitin complexes. affinity chromatography heat-shock immunoprecipitation thioester Abbreviations used: E1, ubiquitin-activating enzyme; E2, ubiquitin-conjugating enzyme; E3, ubiquitin-protein ligase; MUCRP1, multi-ubiquitin chain...

... Sepharose as a probe for site-specific S-glutathionylation and as a novel and potentially useful tool to isolate and identify proteins which are candidate targets for GSNO-induced mixed- disulphide formation. Key words: affinity chromatography, cysteine, glutathionylation, nitric oxide, nitrosothiol...

... -dodecyl β- D -maltoside-solubilized microsomes and their purifications by affinity chromatography on Reactive Red column reveal that inactivation is due to two main effects. (i) Solubilization activates an aspartic protease that cleaves down the α-subunit of the H + ,K + -ATPase. Addition of pepstatin...

... ). 28 1 1999 6 4 1999 30 4 1999 The Biochemical Society, London © 1999 1999 affinity chromatography glycosylation hybridoma secretory component (SC)IgA interaction Biochem. J. (1999) 341, 299 306 (Printed in Great Britain) 299 Expression, purification and biochemical...

... IgE-mediated degranulation of mast cells, as molecular probes in affinity chromatography. All of these drugs, which have different structures but similar function, scarcely bound to calmodulin in bovine lung extract, but bound to the same kinds of calcium-binding proteins, such as the 10-kDa proteins...

...Sabine PINGEL; Uta RHEINWEILER; Volker KOLB; Michael DUSZENKO A membrane-associated galactosyltransferase from Trypanosoma brucei was purified 34000-fold by affinity chromatography on UDP-hexanolamine–Sepharose™. Using SDS/PAGE under reducing conditions, the isolated enzyme ran as a relatively...