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Keywords: Saccharomyces cerevisiae
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Articles
Biochem J (2021) 478 (24): 4153–4167.
Published: 16 December 2021
... for this article was enabled by the participation of University of Liverpool in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. aging mitochondria molecular chaperones Saccharomyces cerevisiae Dietary...
Includes: Supplementary data
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Biochem J (2020) 477 (18): 3613–3623.
Published: 28 September 2020
...Pierre Voisin; Marianne Bernard; Thierry Bergès; Matthieu Régnacq Lipid droplets are ubiquitous organelles in eukaryotes that act as storage sites for neutral lipids. Under normal growth conditions, they are not required in the yeast Saccharomyces cerevisiae . However, recent works have shown...
Includes: Supplementary data
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Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
... been shown to increase the production of fatty acid esters, fatty alcohols, waxes and TAGs in Escherichia coli and yeast ( Saccharomyces cerevisiae ). For example, metabolic engineering of E. coli to produce fatty acid esters, fatty alcohols and waxes was achieved by overexpression of LACS...
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Biochem J (2018) 475 (17): 2831–2845.
Published: 11 September 2018
...: Cheol-Won Yun ( cheolwony@korea.ac.kr ) 29 5 2018 26 7 2018 1 8 2018 2 8 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 AfMac1 A. fumigatus copper iron Saccharomyces cerevisiae Copper and iron...
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Biochem J (2018) 475 (16): 2637–2652.
Published: 29 August 2018
...Wataru Nomura; Miho Aoki; Yoshiharu Inoue Dihydroxyacetone (DHA) is the smallest ketotriose, and it is utilized by many organisms as an energy source. However, at higher concentrations, DHA becomes toxic towards several organisms including the budding yeast Saccharomyces cerevisiae . In the present...
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Biochem J (2018) 475 (8): 1473–1489.
Published: 30 April 2018
... to form acyl-CoA, which in turn may serve as an acyl-donor in the DGAT-catalyzed reaction leading to TAG. To test this hypothesis, flax LACS and DGAT cDNAs were functionally expressed in Saccharomyces cerevisiae strains to probe their possible involvement in the enrichment of TAG with ALA. Among...
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Biochem J (2017) 474 (20): 3439–3454.
Published: 05 October 2017
... of TorA, the biological function of TorA remains to be established. Here, we use the yeast Saccharomyces cerevisiae as a tractable in vivo model to explore TorA function. We demonstrate that TorA can protect yeast cells against different forms of environmental stress and show that in the absence...
Includes: Supplementary data
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Biochem J (2017) 474 (11): 1807–1821.
Published: 16 May 2017
... Society 2017 oligopeptide transporter family Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport The electrochemical proton gradient acts as the driving force for the uptake and efflux of many metabolites and ions across the plasma membrane of fungi...
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Biochem J (2017) 474 (7): 1175–1193.
Published: 15 March 2017
... of glutathionylated proteins in the yeast Saccharomyces cerevisiae and present a detailed proteomic analysis of the targets of protein glutathionylation in cells undergoing constitutive metabolism and after exposure to various stress conditions. This work establishes the physiological importance...
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Biochem J (2017) 474 (2): 301–315.
Published: 06 January 2017
... that three metal-based compounds {[Fe(HPClNOL)Cl 2 ]NO 3 , [Cu(HPClNOL)(CH 3 CN)](ClO 4 ) 2 and Mn(HPClNOL)(Cl) 2 }, harboring in vitro SOD and/or CAT activities, were critical for protection of yeast cells against oxidative stress. In this work, treating Saccharomyces cerevisiae with these SOD/CAT mimics...
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Biochem J (2017) 474 (1): 51–63.
Published: 22 December 2016
... © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 contact site ER stress lipid asymmetry plasma membrane Rim101 pathway Saccharomyces cerevisiae Living organisms must respond appropriately to environmental changes. For instance...
Articles
Biochem J (2016) 473 (19): 3065–3079.
Published: 27 September 2016
... 29 7 2016 1 8 2016 1 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 Hot1 Saccharomyces cerevisiae Spt4/5 Sub1 tandem affinity purification transcription To analyze Hot1, Sub1, Spt5 or Yra1 binding...
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Biochem J (2016) 473 (15): 2369–2382.
Published: 28 July 2016
...Mohammad Zulkifli; Shambhu Yadav; Anil Thakur; Shiffalli Singla; Monika Sharma; Anand Kumar Bachhawat The high-affinity glutathione transporter Hgt1p of Saccharomyces cerevisiae belongs to a relatively new and structurally uncharacterized oligopeptide transporter (OPT) family. To understand...
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Biochem J (2016) 473 (9): 1203–1213.
Published: 26 April 2016
... are putative virulence factors of A. fumigatus ; sit1 and sit2 are homologous to yeast Sit1, and sit1 and sit2 gene expression was up-regulated after iron depletion. When expressed heterologously in Saccharomyces cerevisiae , sit1 and sit2 were localized to the plasma membrane ; sit1 efficiently complemented...
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Biochem J (2015) 466 (3): 547–559.
Published: 06 March 2015
...), an essential process for overcoming starvation. In Saccharomyces cerevisiae , sensing amino acid shortages requires that Gcn2 binds directly to its effector protein Gcn1 and both must associate with the ribosome. Our hypothesis is that uncharged tRNAs occur in the ribosomal A-site and that Gcn1 is directly...
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Biochem J (2015) 466 (2): 283–290.
Published: 20 February 2015
... that synthesis of trehalose-6-phosphate might not be the only mechanism by which AtTPS1 affects plant growth and development. Arabidopsis thaliana Saccharomyces cerevisiae trehalose trehalose phosphatase (TPP) trehalose 6-phosphate trehalose-6-phosphate synthase (TPS) Trehalose is a non...
Articles
Biochem J (2014) 462 (3): 567–579.
Published: 22 August 2014
...Clara Andrea Solari; Vanesa Tudisca; Marcelo Pugliessi; Alejandro Daniel Nadra; Silvia Moreno; Paula Portela PKA (cAMP-dependent protein kinase) activity, as well as that of other AGC members, is regulated by multiple phosphorylations of its catalytic subunits. In Saccharomyces cerevisiae , the PKA...
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Biochem J (2014) 462 (1): 185–197.
Published: 24 July 2014
... membrane-bound proton-translocating V o complex. This nutrient-dependent phenomenon had been first detected in the midgut epithelium of non-feeding moulting tobacco hornworms ( Manduca sexta ) and in glucose-deprived yeast cells ( Saccharomyces cerevisiae ). Since reversible disassembly to date had been...
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Biochem J (2014) 457 (3): 391–400.
Published: 10 January 2014
...Hyun-Jun Kang; Sook-Jin Jeong; Kyung-Nam Kim; In-Joon Baek; Miwha Chang; Chang-Min Kang; Yong-Sung Park; Cheol-Won Yun The homologue of human YTHDF2 , Ydr374c (Pho92), is the only protein that has a YTH (YT521-B homology) domain in Saccharomyces cerevisiae . Based on microarray analysis, genes...
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Biochem J (2013) 454 (3): 551–558.
Published: 29 August 2013
... 2012 12 6 2013 21 6 2013 21 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 ammonia production Jen1p protein monocarboxylic acid import Saccharomyces cerevisiae yeast colony development Yeast colonies growing on solid medium periodically...
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Biochem J (2012) 448 (3): 307–320.
Published: 21 November 2012
... of the PKA catalytic subunits on to the PIF (PDK1-interacting fragment) pocket of PDK1 is a critical step in this activation process. However, PDK1 regulation of PKA in vivo remains controversial. Saccharomyces cerevisiae contains three PKA catalytic subunits, TPK1 , TPK2 and TPK3 . We demonstrate that Pkh...
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Biochem J (2012) 446 (2): 225–233.
Published: 14 August 2012
... granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca 2+ level in Saccharomyces cerevisiae . Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused...
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Biochem J (2012) 445 (3): 413–422.
Published: 13 July 2012
...Dieter R. Samyn; Lorena Ruiz-Pávon; Michael R. Andersson; Yulia Popova; Johan M. Thevelein; Bengt L. Persson In Saccharomyces cerevisiae , the Pho84 phosphate transporter acts as the main provider of phosphate to the cell using a proton symport mechanism, but also mediates rapid activation...
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Biochem J (2012) 443 (3): 663–670.
Published: 16 April 2012
... between Saccharomyces cerevisiae Nth1 and 14-3-3 protein isoforms Bmh1 and Bmh2. We determined four residues that are phosphorylated by PKA (protein kinase A) in vitro within the disordered N-terminal segment of Nth1. Sedimentation analysis and enzyme kinetics measurements show that both yeast 14-3-3...
Includes: Supplementary data
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Biochem J (2012) 442 (2): 357–368.
Published: 13 February 2012
...J. Albert Abrie; Asier González; Erick Strauss; Joaquín Ariño The Saccharomyces cerevisiae Hal3 protein is a moonlighting protein, able to function both as an inhibitory subunit of the Ppz1 protein phosphatase and as a constituent protomer of an unprecedented heterotrimeric PPCDC...
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Biochem J (2012) 441 (1): 487–498.
Published: 14 December 2011
... to six rounds of directed evolution, achieving a level of secretion in Saccharomyces cerevisiae (21 mg/l) as yet unseen for any ligninolytic peroxidase. The evolved variant for expression harboured four mutations and increased its total VP activity 129-fold. The signal leader processing by the STE13...
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Biochem J (2012) 441 (1): 255–264.
Published: 14 December 2011
.... 1 To whom correspondence should be addressed (email rserrano@ibmcp.upv.es ). 14 7 2011 12 9 2011 15 9 2011 15 9 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 pH homoeostasis signal transduction Gcn2 Saccharomyces cerevisiae amino...
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Biochem J (2011) 440 (2): 195–202.
Published: 14 November 2011
... resistance multidrug resistance pleiotropic drug resistance Saccharomyces cerevisiae MDR (multidrug resistance), the ability to acquire simultaneous resistance to unrelated chemical compounds, is a widespread phenomenon that often results from the activity of multidrug efflux pumps of the ABC...
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Biochem J (2011) 438 (3): 523–533.
Published: 26 August 2011
...Carlos Casado; Asier González; Maria Platara; Amparo Ruiz; Joaquín Ariño Exposure of Saccharomyces cerevisiae to alkaline pH provokes a stress condition that generates a compensatory reaction. In the present study we examined a possible role for the PKA (protein kinase A) pathway in this response...
Includes: Supplementary data
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Biochem J (2011) 437 (2): 269–278.
Published: 28 June 2011
...-translocating inorganic pyrophosphatase Saccharomyces cerevisiae vacuolar-type ATPase The occurrence of an electrochemical proton gradient across the internal membranes of eukaryotic cells is one of the known requirements for the correct function of the endo- and exo-cytic pathways [ 1 ]. Moreover...
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Biochem J (2011) 436 (1): 83–90.
Published: 27 April 2011
... together, this implies that Taf14 is involved in transcriptional activation of Saccharomyces cerevisiae and the YEATS domain of Taf14 might play a negative role in cell growth. 1 To whom correspondence should be addressed (email xmtu@ustc.edu.cn ). The structural co-ordinates reported...
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Biochem J (2011) 435 (1): 259–266.
Published: 15 March 2011
...Yi-Hsuan Wu; Avery G. Frey; David J. Eide The Msc2 and Zrg17 proteins of Saccharomyces cerevisiae are members of the cation diffusion facilitator family of zinc transporters. These proteins form heteromeric complexes that transport zinc into the ER (endoplasmic reticulum). Previous studies...
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Biochem J (2011) 434 (1): 161–170.
Published: 27 January 2011
... discovered a PtdIns3 P -synthesizing activity in peroxisomes of Saccharomyces cerevisiae and have demonstrated that the lipid kinase Vps34p is already associated with peroxisomes during biogenesis. However, although Vps34 is required, it is not essential for optimal peroxisome biogenesis. The function...
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Biochem J (2010) 432 (3): 595–608.
Published: 25 November 2010
...María Guirola; Lina Barreto; Ayelen Pagani; Miriam Romagosa; Antonio Casamayor; Silvia Atrian; Joaquín Ariño The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1 Δ strain...
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Biochem J (2010) 432 (3): 445–454.
Published: 25 November 2010
... pyridoxal 5-phosphate synthase Saccharomyces cerevisiae site-directed mutagenesis The active form of vitamin B6, PLP (pyridoxal 5-phosphate), is an essential cofactor for numerous metabolic enzymes. PLP also has an important role in amino acid and carbohydrate metabolism [ 1 , 2 ]. To date, two...
Includes: Supplementary data
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Biochem J (2010) 431 (2): 257–265.
Published: 28 September 2010
...Dong-Hyuk Heo; In-Joon Baek; Hyun-Jun Kang; Ji-Hyun Kim; Miwha Chang; Mi-Young Jeong; Tae-Hyoung Kim; Il-Dong Choi; Cheol-Won Yun Cadmium is a toxic metal and the mechanism of its toxicity has been studied in various model systems from bacteria to mammals. We employed Saccharomyces cerevisiae...
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Biochem J (2010) 431 (1): 31–38.
Published: 14 September 2010
...L. Ashley Cowart; Jason L. Gandy; Baby Tholanikunnel; Yusuf A. Hannun Recent work, especially in the yeast Saccharomyces cerevisiae , has demonstrated that mRNA movement from active translation to cytoplasmic granules, termed mRNA‘p-bodies’ (processing bodies), occurs in concert with the regulation...
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Biochem J (2010) 426 (2): 205–217.
Published: 09 February 2010
... compilation © 2010 Biochemical Society 2010 ALG11 dolichol lipid-linked oligosaccharide mannosyltransferase protein N-glycosylation Saccharomyces cerevisiae The pathway of N-linked protein glycosylation in eukaryotes is highly conserved and starts with the assembly of the common core...
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Biochem J (2010) 426 (2): 147–157.
Published: 09 February 2010
...Rocío Drake; Aurelio Serrano; José R. Pérez-Castiñeira Expression of heterologous multispanning membrane proteins in Saccharomyces cerevisiae is a difficult task. Quite often, the use of multicopy plasmids where the foreign gene is under the control of a strong promoter does not guarantee efficient...
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Biochem J (2010) 425 (1): 235–243.
Published: 14 December 2009
...Raquel Castro-Prego; Mónica Lamas-Maceiras; Pilar Soengas; Isabel Carneiro; Isabel González-Siso; M. Esperanza Cerdán Ixr1p from Saccharomyces cerevisiae has been previously studied because it binds to DNA containing intrastrand cross-links formed by the anticancer drug cisplatin. Ixr1p is also...
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Biochem J (2009) 424 (1): 61–67.
Published: 23 October 2009
... that conditions of ER (endoplasmic reticulum) stress stimulate LD formation in Saccharomyces cerevisiae . We found that LDs accumulated in yeast mutants with compromised protein glycosylation or ER-associated protein degradation. Moreover, tunicamycin and Brefeldin A, agents which induce ER stress, were found...
Includes: Supplementary data
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Biochem J (2009) 422 (1): 181–191.
Published: 29 July 2009
...Mi-Young Jeong; Chang-Min Kang; Ji-Hyun Kim; Dong-Hyuk Heo; Miwha Chang; In-Joon Baek; Hyeon-Su Ro; Il-Dong Choi; Tae-Hyoung Kim; Cheol-Won Yun Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron...
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Biochem J (2009) 419 (1): 229–236.
Published: 13 March 2009
.... The SNARE Vti1p is required for several transport steps between late Golgi, endosomes and the vacuole in the yeast Saccharomyces cerevisiae . Here, we identified the late Golgi membrane protein TVP23 as a multicopy suppressor of the growth defect in vti1 - 2 cells. By contrast, the growth defect in vti1...
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Biochem J (2008) 416 (3): 365–374.
Published: 26 November 2008
...Aparna K. Sapra; Piyush Khandelia; Usha Vijayraghavan Saccharomyces cerevisiae PRP17 -null mutants are temperature-sensitive for growth. In vitro splicing with extracts lacking Prp17 are kinetically slow for the first step of splicing and are arrested for the second step at temperatures greater...
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Biochem J (2008) 415 (3): 455–466.
Published: 15 October 2008
... and signalling from the cell surface. A proteome-wide screen performed in Saccharomyces cerevisiae revealed that Ypp1 interacts physically with the plasma-membrane-associated phosphoinositide 4-kinase, Stt4. In the present study, we demonstrate that phenotypes of ypp1 and stt4 conditional mutants are identical...
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Biochem J (2008) 415 (2): 233–239.
Published: 25 September 2008
... (hexokinase 2) from Saccharomyces cerevisiae . In fact, it has been previously described that expression of GK β in yeast could replace Hxk2 in the glucose signalling pathway of S. cerevisiae . In the present study we report that GK β exerts its regulatory role by association with the yeast transcriptional...
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Biochem J (2008) 409 (3): 651–656.
Published: 15 January 2008
... topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA) 35 , lying in chromosome XIII of Saccharomyces cerevisiae , we can conclude that nucleosomes represent a physical barrier for the enzyme activity. 1 To whom correspondence should be addressed (email...
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Biochem J (2008) 409 (2): 399–406.
Published: 21 December 2007
...Tomokazu Ito; Hisashi Hemmi; Kunishige Kataoka; Yukio Mukai; Tohru Yoshimura YGL196W of Saccharomyces cerevisiae encodes a putative protein that is unidentified but is predicted to have a motif similar to that of the N-terminal domain of the bacterial alanine racemase. In the present study we found...
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Biochem J (2008) 409 (1): 299–309.
Published: 11 December 2007
...Thomas Desfougères; Thierry Ferreira; Thierry Bergès; Matthieu Régnacq The yeast Saccharomyces cerevisiae is a facultative anaerobic organism. Under anaerobiosis, sustained growth relies on the presence of exogenously supplied unsaturated fatty acids and ergosterol that yeast is unable...
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Biochem J (2007) 408 (1): 97–104.
Published: 29 October 2007
... with iron permease ScFtr1 and ferroxidase ScFet3 of Saccharomyces cerevisiae at the amino acid level. The genes encoding iron permease and ferroxidase were localized to the same chromosome in the manner of FgFtr1/FgFet1 and FgFtr2/FgFet2. The GFP (green fluorescent protein)-fused versions of FgFtr1...
Includes: Supplementary data