Page 1 | Search Results | Biochemical Journal

1-50 of 90

Sort by

Sort Order Select

Articles

Mitophagy in yeast: known unknowns and unknown unknowns

Hagai Abeliovich

Biochem J (2023) 480 (20): 1639–1657.

https://doi.org/10.1042/BCJ20230279

Published: 18 October 2023

... and muscle cells. Although the mechanistic studies of mitophagy in mammalian cells were initiated after the initial mechanistic findings in Saccharomyces cerevisiae , our current understanding of the physiological role of mitophagy in yeast remains more limited, despite the presence of better-defined assays...

View article titled, Mitophagy in yeast: known unknowns and unknown unknowns

Articles

Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Richard Campion, Leanne Bloxam, Kimberley Burrow, Philip J. Brownridge, Daniel R. Pentland, Patricia Thomas, Campbell W. Gourlay, Claire E. Eyers, Jeff W. Barclay, Alan Morgan

Biochem J (2021) 478 (24): 4153–4167.

https://doi.org/10.1042/BCJ20210432

Published: 16 December 2021

... for this article was enabled by the participation of University of Liverpool in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. aging mitochondria molecular chaperones Saccharomyces cerevisiae Dietary...

View article titled, Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Includes: Supplementary data

Articles

Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Pierre Voisin, Marianne Bernard, Thierry Bergès, Matthieu Régnacq

Biochem J (2020) 477 (18): 3613–3623.

https://doi.org/10.1042/BCJ20200551

Published: 28 September 2020

...Pierre Voisin; Marianne Bernard; Thierry Bergès; Matthieu Régnacq Lipid droplets are ubiquitous organelles in eukaryotes that act as storage sites for neutral lipids. Under normal growth conditions, they are not required in the yeast Saccharomyces cerevisiae . However, recent works have shown...

View article titled, Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Includes: Supplementary data

Articles

Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Yang Xu, Kristian Mark P. Caldo, Roman Holic, Elzbieta Mietkiewska, Jocelyn Ozga, Syed Masood Rizvi, Guanqun Chen, Randall J. Weselake

Biochem J (2019) 476 (1): 151–164.

https://doi.org/10.1042/BCJ20180787

Published: 15 January 2019

... been shown to increase the production of fatty acid esters, fatty alcohols, waxes and TAGs in Escherichia coli and yeast ( Saccharomyces cerevisiae ). For example, metabolic engineering of E. coli to produce fatty acid esters, fatty alcohols and waxes was achieved by overexpression of LACS...

View article titled, Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Includes: Supplementary data

Articles

A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Yong-Sung Park, Suzie Kang, Hyewon Seo, Cheol-Won Yun

Biochem J (2018) 475 (17): 2831–2845.

https://doi.org/10.1042/BCJ20180399

Published: 11 September 2018

...: Cheol-Won Yun ( cheolwony@korea.ac.kr ) Saccharomyces cerevisiae has been used to study iron and copper metabolism. In S. cerevisiae , reduced transition metals from the environment are transported into cytoplasmic compartments by metal-specific transporters [ 5 – 7 ]. Both reductive and non...

View article titled, A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Includes: Supplementary data

Articles

Toxicity of dihydroxyacetone is exerted through the formation of methylglyoxal in Saccharomyces cerevisiae : effects on actin polarity and nuclear division

Wataru Nomura, Miho Aoki, Yoshiharu Inoue

Biochem J (2018) 475 (16): 2637–2652.

https://doi.org/10.1042/BCJ20180234

Published: 29 August 2018

...Wataru Nomura; Miho Aoki; Yoshiharu Inoue Dihydroxyacetone (DHA) is the smallest ketotriose, and it is utilized by many organisms as an energy source. However, at higher concentrations, DHA becomes toxic towards several organisms including the budding yeast Saccharomyces cerevisiae . In the present...

View article titled, Toxicity of dihydroxyacetone is exerted through the formation of methylglyoxal in Saccharomyces cerevisiae : effects on actin polarity and nuclear division

Includes: Supplementary data

Articles

Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with α-linolenic acid

Yang Xu, Roman Holic, Darren Li, Xue Pan, Elzbieta Mietkiewska, Guanqun Chen, Jocelyn Ozga, Randall J. Weselake

Biochem J (2018) 475 (8): 1473–1489.

https://doi.org/10.1042/BCJ20170910

Published: 30 April 2018

... to form acyl-CoA, which in turn may serve as an acyl-donor in the DGAT-catalyzed reaction leading to TAG. To test this hypothesis, flax LACS and DGAT cDNAs were functionally expressed in Saccharomyces cerevisiae strains to probe their possible involvement in the enrichment of TAG with ALA. Among...

View article titled, Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with α-linolenic acid

Includes: Supplementary data

Articles

Human TorsinA can function in the yeast cytosol as a molecular chaperone

Ilectra Adam, Lyne Jossé, Mick F. Tuite

Biochem J (2017) 474 (20): 3439–3454.

https://doi.org/10.1042/BCJ20170395

Published: 05 October 2017

... of TorA, the biological function of TorA remains to be established. Here, we use the yeast Saccharomyces cerevisiae as a tractable in vivo model to explore TorA function. We demonstrate that TorA can protect yeast cells against different forms of environmental stress and show that in the absence...

View article titled, Human TorsinA can function in the yeast cytosol as a molecular chaperone

Includes: Supplementary data

Articles

Identification of residues critical for proton-coupled glutathione translocation in the yeast glutathione transporter, Hgt1p

Mohammad Zulkifli, Anand Kumar Bachhawat

Biochem J (2017) 474 (11): 1807–1821.

https://doi.org/10.1042/BCJ20161063

Published: 16 May 2017

... Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport The electrochemical proton gradient acts as the driving force for the uptake and efflux of many metabolites and ions across the plasma membrane of fungi and plants [ 1 , 2 ]. A large number of transporters...

View article titled, Identification of residues critical for proton-coupled glutathione translocation in the yeast glutathione transporter, Hgt1p

Includes: Supplementary data

Articles

Modulation of the specific glutathionylation of mitochondrial proteins in the yeast Saccharomyces cerevisiae under basal and stress conditions

Rachel Gergondey, Camille Garcia, Christophe H. Marchand, Stephane D. Lemaire, Jean-Michel Camadro, Françoise Auchère

Biochem J (2017) 474 (7): 1175–1193.

https://doi.org/10.1042/BCJ20160927

Published: 15 March 2017

... of glutathionylated proteins in the yeast Saccharomyces cerevisiae and present a detailed proteomic analysis of the targets of protein glutathionylation in cells undergoing constitutive metabolism and after exposure to various stress conditions. This work establishes the physiological importance...

View article titled, Modulation of the specific glutathionylation of mitochondrial proteins in the yeast Saccharomyces cerevisiae under basal and stress conditions

Includes: Supplementary data

Articles

Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae

Thales de P. Ribeiro, Fernanda L. Fonseca, Mariana D.C. de Carvalho, Rodrigo M. da C. Godinho, Fernando Pereira de Almeida, Tatiana D. Saint'Pierre, Nicolás A. Rey, Christiane Fernandes, Adolfo Horn, Jr, Marcos D. Pereira

Biochem J (2017) 474 (2): 301–315.

https://doi.org/10.1042/BCJ20160480

Published: 06 January 2017

... that three metal-based compounds {[Fe(HPClNOL)Cl 2 ]NO 3 , [Cu(HPClNOL)(CH 3 CN)](ClO 4 ) 2 and Mn(HPClNOL)(Cl) 2 }, harboring in vitro SOD and/or CAT activities, were critical for protection of yeast cells against oxidative stress. In this work, treating Saccharomyces cerevisiae with these SOD/CAT mimics...

View article titled, Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae

Includes: Supplementary data

Articles

The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

Keisuke Obara, Akio Kihara

Biochem J (2017) 474 (1): 51–63.

https://doi.org/10.1042/BCJ20160580

Published: 22 December 2016

... plasma membrane Rim101 pathway Saccharomyces cerevisiae Living organisms must respond appropriately to environmental changes. For instance, fungi that grow over a wide pH range, such as Saccharomyces cerevisiae , Candida albicans , and Aspergillus nidulans , adapt to alkaline conditions...

View article titled, The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

Articles

Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes

M. Gomar-Alba, M. del Olmo

Biochem J (2016) 473 (19): 3065–3079.

https://doi.org/10.1042/BCJ20160463

Published: 27 September 2016

... Saccharomyces cerevisiae Spt4/5 Sub1 tandem affinity purification transcription Changes in the extracellular environment force microorganisms to develop mechanisms of adaptation and survival. Under high osmolarity conditions, the synthesis and retention of compatible osmolyte glycerol is essential...

View article titled, Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes

Includes: Supplementary data

Articles

Substrate specificity and mapping of residues critical for transport in the high-affinity glutathione transporter Hgt1p

Mohammad Zulkifli, Shambhu Yadav, Anil Thakur, Shiffalli Singla, Monika Sharma, Anand Kumar Bachhawat

Biochem J (2016) 473 (15): 2369–2382.

https://doi.org/10.1042/BCJ20160231

Published: 28 July 2016

...Mohammad Zulkifli; Shambhu Yadav; Anil Thakur; Shiffalli Singla; Monika Sharma; Anand Kumar Bachhawat The high-affinity glutathione transporter Hgt1p of Saccharomyces cerevisiae belongs to a relatively new and structurally uncharacterized oligopeptide transporter (OPT) family. To understand...

View article titled, Substrate specificity and mapping of residues critical for transport in the high-affinity glutathione transporter Hgt1p

Includes: Supplementary data

Articles

Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by Aspergillus fumigatus

Yong-Sung Park, Ju-Yeon Kim, Cheol-Won Yun

Biochem J (2016) 473 (9): 1203–1213.

https://doi.org/10.1042/BCJ20160066

Published: 26 April 2016

... are putative virulence factors of A. fumigatus ; sit1 and sit2 are homologous to yeast Sit1, and sit1 and sit2 gene expression was up-regulated after iron depletion. When expressed heterologously in Saccharomyces cerevisiae , sit1 and sit2 were localized to the plasma membrane ; sit1 efficiently complemented...

View article titled, Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by Aspergillus fumigatus

Includes: Supplementary data

Articles

Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2

Su Jung Lee, Mark J. Swanson, Evelyn Sattlegger

Biochem J (2015) 466 (3): 547–559.

https://doi.org/10.1042/BJ20140782

Published: 06 March 2015

...), an essential process for overcoming starvation. In Saccharomyces cerevisiae , sensing amino acid shortages requires that Gcn2 binds directly to its effector protein Gcn1 and both must associate with the ribosome. Our hypothesis is that uncharged tRNAs occur in the ribosomal A-site and that Gcn1 is directly...

View article titled, Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2

Articles

Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana

Ines Delorge, Carlos M. Figueroa, Regina Feil, John E. Lunn, Patrick Van Dijck

Biochem J (2015) 466 (2): 283–290.

https://doi.org/10.1042/BJ20141322

Published: 20 February 2015

...-phosphate might not be the only mechanism by which AtTPS1 affects plant growth and development. Arabidopsis thaliana Saccharomyces cerevisiae trehalose trehalose phosphatase (TPP) trehalose 6-phosphate trehalose-6-phosphate synthase (TPS) Trehalose is a non-reducing disaccharide...

View article titled, Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana

Articles

Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae

Clara Andrea Solari, Vanesa Tudisca, Marcelo Pugliessi, Alejandro Daniel Nadra, Silvia Moreno, Paula Portela

Biochem J (2014) 462 (3): 567–579.

https://doi.org/10.1042/BJ20140577

Published: 22 August 2014

...Clara Andrea Solari; Vanesa Tudisca; Marcelo Pugliessi; Alejandro Daniel Nadra; Silvia Moreno; Paula Portela PKA (cAMP-dependent protein kinase) activity, as well as that of other AGC members, is regulated by multiple phosphorylations of its catalytic subunits. In Saccharomyces cerevisiae , the PKA...

View article titled, Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Reversible disassembly of the yeast V-ATPase revisited under in vivo conditions

Katharina Tabke, Andrea Albertmelcher, Olga Vitavska, Markus Huss, Hans-Peter Schmitz, Helmut Wieczorek

Biochem J (2014) 462 (1): 185–197.

https://doi.org/10.1042/BJ20131293

Published: 24 July 2014

... membrane-bound proton-translocating V o complex. This nutrient-dependent phenomenon had been first detected in the midgut epithelium of non-feeding moulting tobacco hornworms ( Manduca sexta ) and in glucose-deprived yeast cells ( Saccharomyces cerevisiae ). Since reversible disassembly to date had been...

View article titled, Reversible disassembly of the yeast V-ATPase revisited under in vivo conditions

Articles

A novel protein, Pho92, has a conserved YTH domain and regulates phosphate metabolism by decreasing the mRNA stability of PHO4 in Saccharomyces cerevisiae

Hyun-Jun Kang, Sook-Jin Jeong, Kyung-Nam Kim, In-Joon Baek, Miwha Chang, Chang-Min Kang, Yong-Sung Park, Cheol-Won Yun

Biochem J (2014) 457 (3): 391–400.

https://doi.org/10.1042/BJ20130862

Published: 10 January 2014

...Hyun-Jun Kang; Sook-Jin Jeong; Kyung-Nam Kim; In-Joon Baek; Miwha Chang; Chang-Min Kang; Yong-Sung Park; Cheol-Won Yun The homologue of human YTHDF2 , Ydr374c (Pho92), is the only protein that has a YTH (YT521-B homology) domain in Saccharomyces cerevisiae . Based on microarray analysis, genes...

View article titled, A novel protein, Pho92, has a conserved YTH domain and regulates phosphate metabolism by decreasing the mRNA stability of PHO4 in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies

Sandra Paiva, Dita Strachotová, Helena Kučerová, Otakar Hlaváček, Sandra Mota, Margarida Casal, Zdena Palková, Libuše Váchová

Biochem J (2013) 454 (3): 551–558.

https://doi.org/10.1042/BJ20120312

Published: 29 August 2013

..., in which cell dying occurs throughout the whole colony. ammonia production Jen1p protein monocarboxylic acid import Saccharomyces cerevisiae yeast colony development Yeast colonies growing on solid medium periodically acidify and alkalize their surroundings. The alkali developmental...

View article titled, The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies

Articles

The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae

Steven Haesendonckx, Vanesa Tudisca, Karin Voordeckers, Silvia Moreno, Johan M. Thevelein, Paula Portela

Biochem J (2012) 448 (3): 307–320.

https://doi.org/10.1042/BJ20121061

Published: 21 November 2012

... of the PKA catalytic subunits on to the PIF (PDK1-interacting fragment) pocket of PDK1 is a critical step in this activation process. However, PDK1 regulation of PKA in vivo remains controversial. Saccharomyces cerevisiae contains three PKA catalytic subunits, TPK1 , TPK2 and TPK3 . We demonstrate that Pkh...

View article titled, The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae

Aya Iwaki, Shingo Izawa

Biochem J (2012) 446 (2): 225–233.

https://doi.org/10.1042/BJ20120583

Published: 14 August 2012

... granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca 2+ level in Saccharomyces cerevisiae . Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused...

View article titled, Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Mutational analysis of putative phosphate- and proton-binding sites in the Saccharomyces cerevisiae Pho84 phosphate:H + transceptor and its effect on signalling to the PKA and PHO pathways

Dieter R. Samyn, Lorena Ruiz-Pávon, Michael R. Andersson, Yulia Popova, Johan M. Thevelein, Bengt L. Persson

Biochem J (2012) 445 (3): 413–422.

https://doi.org/10.1042/BJ20112086

Published: 13 July 2012

...Dieter R. Samyn; Lorena Ruiz-Pávon; Michael R. Andersson; Yulia Popova; Johan M. Thevelein; Bengt L. Persson In Saccharomyces cerevisiae , the Pho84 phosphate transporter acts as the main provider of phosphate to the cell using a proton symport mechanism, but also mediates rapid activation...

View article titled, Mutational analysis of putative phosphate- and proton-binding sites in the Saccharomyces cerevisiae Pho84 phosphate:H + transceptor and its effect on signalling to the PKA and PHO pathways

Includes: Supplementary data

Articles

Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1

Dana Veisova, Eva Macakova, Lenka Rezabkova, Miroslav Sulc, Petr Vacha, Hana Sychrova, Tomas Obsil, Veronika Obsilova

Biochem J (2012) 443 (3): 663–670.

https://doi.org/10.1042/BJ20111615

Published: 16 April 2012

... between Saccharomyces cerevisiae Nth1 and 14-3-3 protein isoforms Bmh1 and Bmh2. We determined four residues that are phosphorylated by PKA (protein kinase A) in vitro within the disordered N-terminal segment of Nth1. Sedimentation analysis and enzyme kinetics measurements show that both yeast 14-3-3...

View article titled, Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1

Includes: Supplementary data

Articles

Functional mapping of the disparate activities of the yeast moonlighting protein Hal3

J. Albert Abrie, Asier González, Erick Strauss, Joaquín Ariño

Biochem J (2012) 442 (2): 357–368.

https://doi.org/10.1042/BJ20111466

Published: 13 February 2012

...J. Albert Abrie; Asier González; Erick Strauss; Joaquín Ariño The Saccharomyces cerevisiae Hal3 protein is a moonlighting protein, able to function both as an inhibitory subunit of the Ppz1 protein phosphatase and as a constituent protomer of an unprecedented heterotrimeric PPCDC...

View article titled, Functional mapping of the disparate activities of the yeast moonlighting protein Hal3

Includes: Supplementary data

Articles

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase

Eva Garcia-Ruiz, David Gonzalez-Perez, Francisco J. Ruiz-Dueñas, Angel T. Martínez, Miguel Alcalde

Biochem J (2012) 441 (1): 487–498.

https://doi.org/10.1042/BJ20111199

Published: 14 December 2011

... to six rounds of directed evolution, achieving a level of secretion in Saccharomyces cerevisiae (21 mg/l) as yet unseen for any ligninolytic peroxidase. The evolved variant for expression harboured four mutations and increased its total VP activity 129-fold. The signal leader processing by the STE13...

View article titled, Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase

Includes: Supplementary data

Articles

A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

Guillem Hueso, Rafael Aparicio-Sanchis, Consuelo Montesinos, Silvia Lorenz, José R. Murguía, Ramón Serrano

Biochem J (2012) 441 (1): 255–264.

https://doi.org/10.1042/BJ20111264

Published: 14 December 2011

.... 1 To whom correspondence should be addressed (email rserrano@ibmcp.upv.es ). 14 7 2011 12 9 2011 15 9 2011 15 9 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 pH homoeostasis signal transduction Gcn2 Saccharomyces cerevisiae amino...

View article titled, A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

Articles

The yeast ABC transporter Pdr18 (ORF YNR070w ) controls plasma membrane sterol composition, playing a role in multidrug resistance

Tânia R. Cabrito, Miguel C. Teixeira, Ashutosh Singh, Rajendra Prasad, Isabel Sá-Correia

Biochem J (2011) 440 (2): 195–202.

https://doi.org/10.1042/BJ20110876

Published: 14 November 2011

... non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. ergosterol homoeostasis herbicide resistance multidrug resistance pleiotropic drug resistance Saccharomyces cerevisiae MDR (multidrug resistance), the ability to acquire...

View article titled, The yeast ABC transporter Pdr18 (ORF YNR070w ) controls plasma membrane sterol composition, playing a role in multidrug resistance

Articles

The role of the protein kinase A pathway in the response to alkaline pH stress in yeast

Carlos Casado, Asier González, Maria Platara, Amparo Ruiz, Joaquín Ariño

Biochem J (2011) 438 (3): 523–533.

https://doi.org/10.1042/BJ20110607

Published: 26 August 2011

...Carlos Casado; Asier González; Maria Platara; Amparo Ruiz; Joaquín Ariño Exposure of Saccharomyces cerevisiae to alkaline pH provokes a stress condition that generates a compensatory reaction. In the present study we examined a possible role for the PKA (protein kinase A) pathway in this response...

View article titled, The role of the protein kinase A pathway in the response to alkaline pH stress in yeast

Includes: Supplementary data

Articles

A plant proton-pumping inorganic pyrophosphatase functionally complements the vacuolar ATPase transport activity and confers bafilomycin resistance in yeast

José R. Pérez-Castiñeira, Agustín Hernández, Rocío Drake, Aurelio Serrano

Biochem J (2011) 437 (2): 269–278.

https://doi.org/10.1042/BJ20110447

Published: 28 June 2011

...-translocating inorganic pyrophosphatase Saccharomyces cerevisiae vacuolar-type ATPase The YPC3 mutant strain was generated from the S. cerevisiae haploid strain W303-1A (MATa, ade2-1 can1-100 his3-11 , 15 leu2-3 , 112 trp1-1 , ura3-1 ) by the single-step transplacement procedure as described...

View article titled, A plant proton-pumping inorganic pyrophosphatase functionally complements the vacuolar ATPase transport activity and confers bafilomycin resistance in yeast

Includes: Supplementary data

Articles

Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae

Wen Zhang, Jiahai Zhang, Xuecheng Zhang, Chao Xu, Xiaoming Tu

Biochem J (2011) 436 (1): 83–90.

https://doi.org/10.1042/BJ20110004

Published: 27 April 2011

... together, this implies that Taf14 is involved in transcriptional activation of Saccharomyces cerevisiae and the YEATS domain of Taf14 might play a negative role in cell growth. All NMR data were collected at 298 K on a Bruker DMX500 spectrometer. A set of standard triple-resonance spectra...

View article titled, Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Transcriptional regulation of the Zrg17 zinc transporter of the yeast secretory pathway

Yi-Hsuan Wu, Avery G. Frey, David J. Eide

Biochem J (2011) 435 (1): 259–266.

https://doi.org/10.1042/BJ20102003

Published: 15 March 2011

...Yi-Hsuan Wu; Avery G. Frey; David J. Eide The Msc2 and Zrg17 proteins of Saccharomyces cerevisiae are members of the cation diffusion facilitator family of zinc transporters. These proteins form heteromeric complexes that transport zinc into the ER (endoplasmic reticulum). Previous studies...

View article titled, Transcriptional regulation of the Zrg17 zinc transporter of the yeast secretory pathway

Articles

The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae

Silke Grunau, Dorothee Lay, Sabrina Mindthoff, Harald W. Platta, Wolfgang Girzalsky, Wilhelm W. Just, Ralf Erdmann

Biochem J (2011) 434 (1): 161–170.

https://doi.org/10.1042/BJ20101115

Published: 27 January 2011

... discovered a PtdIns3 P -synthesizing activity in peroxisomes of Saccharomyces cerevisiae and have demonstrated that the lipid kinase Vps34p is already associated with peroxisomes during biogenesis. However, although Vps34 is required, it is not essential for optimal peroxisome biogenesis. The function...

View article titled, The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae

Articles

Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast

María Guirola, Lina Barreto, Ayelen Pagani, Miriam Romagosa, Antonio Casamayor, Silvia Atrian, Joaquín Ariño

Biochem J (2010) 432 (3): 595–608.

https://doi.org/10.1042/BJ20101032

Published: 25 November 2010

...María Guirola; Lina Barreto; Ayelen Pagani; Miriam Romagosa; Antonio Casamayor; Silvia Atrian; Joaquín Ariño The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1 Δ strain...

View article titled, Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast

Includes: Supplementary data

Articles

Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1

Xuan Zhang, Yan-Bin Teng, Jian-Ping Liu, Yong-Xing He, Kang Zhou, Yuxing Chen, Cong-Zhao Zhou

Biochem J (2010) 432 (3): 445–454.

https://doi.org/10.1042/BJ20101241

Published: 25 November 2010

... Saccharomyces cerevisiae site-directed mutagenesis Compared with the extensively studied DXP-dependent pathway in Escherichia coli , the structural basis for the DXP-independent pathway is poorly understood, despite the availability of structural data on several Pdx1 enzymes. The crystal structure...

View article titled, Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1

Includes: Supplementary data

Articles

Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Dong-Hyuk Heo, In-Joon Baek, Hyun-Jun Kang, Ji-Hyun Kim, Miwha Chang, Mi-Young Jeong, Tae-Hyoung Kim, Il-Dong Choi, Cheol-Won Yun

Biochem J (2010) 431 (2): 257–265.

https://doi.org/10.1042/BJ20100638

Published: 28 September 2010

...Dong-Hyuk Heo; In-Joon Baek; Hyun-Jun Kang; Ji-Hyun Kim; Miwha Chang; Mi-Young Jeong; Tae-Hyoung Kim; Il-Dong Choi; Cheol-Won Yun Cadmium is a toxic metal and the mechanism of its toxicity has been studied in various model systems from bacteria to mammals. We employed Saccharomyces cerevisiae...

View article titled, Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Articles

Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae

L. Ashley Cowart, Jason L. Gandy, Baby Tholanikunnel, Yusuf A. Hannun

Biochem J (2010) 431 (1): 31–38.

https://doi.org/10.1042/BJ20100307

Published: 14 September 2010

...L. Ashley Cowart; Jason L. Gandy; Baby Tholanikunnel; Yusuf A. Hannun Recent work, especially in the yeast Saccharomyces cerevisiae , has demonstrated that mRNA movement from active translation to cytoplasmic granules, termed mRNA‘p-bodies’ (processing bodies), occurs in concert with the regulation...

View article titled, Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae

Articles

N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast

Rocío Drake, Aurelio Serrano, José R. Pérez-Castiñeira

Biochem J (2010) 426 (2): 147–157.

https://doi.org/10.1042/BJ20091491

Published: 09 February 2010

...Rocío Drake; Aurelio Serrano; José R. Pérez-Castiñeira Expression of heterologous multispanning membrane proteins in Saccharomyces cerevisiae is a difficult task. Quite often, the use of multicopy plasmids where the foreign gene is under the control of a strong promoter does not guarantee efficient...

View article titled, N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast

Includes: Supplementary data

Articles

Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae , an α1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide

Birgit Absmanner, Verena Schmeiser, Michael Kämpf, Ludwig Lehle

Biochem J (2010) 426 (2): 205–217.

https://doi.org/10.1042/BJ20091121

Published: 09 February 2010

... oligosaccharide mannosyltransferase protein N-glycosylation Saccharomyces cerevisiae The pathway of N-linked protein glycosylation in eukaryotes is highly conserved and starts with the assembly of the common core oligosaccharide donor Glc 3 Man 9 GlcNAc 2 -PP-Dol, the glycan moiety of which...

View article titled, Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae , an α1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide

Articles

Regulatory factors controlling transcription of Saccharomyces cerevisiae IXR1 by oxygen levels: a model of transcriptional adaptation from aerobiosis to hypoxia implicating ROX1 and IXR1 cross-regulation

Raquel Castro-Prego, Mónica Lamas-Maceiras, Pilar Soengas, Isabel Carneiro, Isabel González-Siso, M. Esperanza Cerdán

Biochem J (2010) 425 (1): 235–243.

https://doi.org/10.1042/BJ20091500

Published: 14 December 2009

...Raquel Castro-Prego; Mónica Lamas-Maceiras; Pilar Soengas; Isabel Carneiro; Isabel González-Siso; M. Esperanza Cerdán Ixr1p from Saccharomyces cerevisiae has been previously studied because it binds to DNA containing intrastrand cross-links formed by the anticancer drug cisplatin. Ixr1p is also...

View article titled, Regulatory factors controlling transcription of Saccharomyces cerevisiae IXR1 by oxygen levels: a model of transcriptional adaptation from aerobiosis to hypoxia implicating ROX1 and IXR1 cross-regulation

Articles

Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae

Weihua Fei, Han Wang, Xin Fu, Christopher Bielby, Hongyuan Yang

Biochem J (2009) 424 (1): 61–67.

https://doi.org/10.1042/BJ20090785

Published: 23 October 2009

... that conditions of ER (endoplasmic reticulum) stress stimulate LD formation in Saccharomyces cerevisiae . We found that LDs accumulated in yeast mutants with compromised protein glycosylation or ER-associated protein degradation. Moreover, tunicamycin and Brefeldin A, agents which induce ER stress, were found...

View article titled, Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Mi-Young Jeong, Chang-Min Kang, Ji-Hyun Kim, Dong-Hyuk Heo, Miwha Chang, In-Joon Baek, Hyeon-Su Ro, Il-Dong Choi, Tae-Hyoung Kim, Cheol-Won Yun

Biochem J (2009) 422 (1): 181–191.

https://doi.org/10.1042/BJ20082399

Published: 29 July 2009

...Mi-Young Jeong; Chang-Min Kang; Ji-Hyun Kim; Dong-Hyuk Heo; Miwha Chang; In-Joon Baek; Hyeon-Su Ro; Il-Dong Choi; Tae-Hyoung Kim; Cheol-Won Yun Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron...

View article titled, A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Articles

TVP23 interacts genetically with the yeast SNARE VTI1 and functions in retrograde transport from the early endosome to the late Golgi

Ivar S. Stein, Anna Gottfried, Jana Zimmermann, Gabriele Fischer von Mollard

Biochem J (2009) 419 (1): 229–236.

https://doi.org/10.1042/BJ20081973

Published: 13 March 2009

.... The SNARE Vti1p is required for several transport steps between late Golgi, endosomes and the vacuole in the yeast Saccharomyces cerevisiae . Here, we identified the late Golgi membrane protein TVP23 as a multicopy suppressor of the growth defect in vti1 - 2 cells. By contrast, the growth defect in vti1...

View article titled, TVP23 interacts genetically with the yeast SNARE VTI1 and functions in retrograde transport from the early endosome to the late Golgi

Articles

The splicing factor Prp17 interacts with the U2, U5 and U6 snRNPs and associates with the spliceosome pre- and post-catalysis

Aparna K. Sapra, Piyush Khandelia, Usha Vijayraghavan

Biochem J (2008) 416 (3): 365–374.

https://doi.org/10.1042/BJ20081195

Published: 26 November 2008

...Aparna K. Sapra; Piyush Khandelia; Usha Vijayraghavan Saccharomyces cerevisiae PRP17 -null mutants are temperature-sensitive for growth. In vitro splicing with extracts lacking Prp17 are kinetically slow for the first step of splicing and are arrested for the second step at temperatures greater...

View article titled, The splicing factor Prp17 interacts with the U2, U5 and U6 snRNPs and associates with the spliceosome pre- and post-catalysis

Includes: Supplementary data

Articles

Ypp1/YGR198w plays an essential role in phosphoinositide signalling at the plasma membrane

Chao Zhai, Kuoyu Li, Valentini Markaki, John P. Phelan, Katherine Bowers, Frank T. Cooke, Barry Panaretou

Biochem J (2008) 415 (3): 455–466.

https://doi.org/10.1042/BJ20080209

Published: 15 October 2008

... and signalling from the cell surface. A proteome-wide screen performed in Saccharomyces cerevisiae revealed that Ypp1 interacts physically with the plasma-membrane-associated phosphoinositide 4-kinase, Stt4. In the present study, we demonstrate that phenotypes of ypp1 and stt4 conditional mutants are identical...

View article titled, Ypp1/YGR198w plays an essential role in phosphoinositide signalling at the plasma membrane

Articles

Human pancreatic β-cell glucokinase: subcellular localization and glucose repression signalling function in the yeast cell

Alberto Riera, Deifilia Ahuatzi, Pilar Herrero, Maria Adelaida Garcia-Gimeno, Pascual Sanz, Fernando Moreno

Biochem J (2008) 415 (2): 233–239.

https://doi.org/10.1042/BJ20080797

Published: 25 September 2008

... (hexokinase 2) from Saccharomyces cerevisiae . In fact, it has been previously described that expression of GK β in yeast could replace Hxk2 in the glucose signalling pathway of S. cerevisiae . In the present study we report that GK β exerts its regulatory role by association with the yeast transcriptional...

View article titled, Human pancreatic β-cell glucokinase: subcellular localization and glucose repression signalling function in the yeast cell

Articles

Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

Francesca Di Felice, Francesco Chiani, Giorgio Camilloni

Biochem J (2008) 409 (3): 651–656.

https://doi.org/10.1042/BJ20070893

Published: 15 January 2008

... topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA) 35 , lying in chromosome XIII of Saccharomyces cerevisiae , we can conclude that nucleosomes represent a physical barrier for the enzyme activity. 1 To whom correspondence should be addressed (email...

View article titled, Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

Articles

A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae

Tomokazu Ito, Hisashi Hemmi, Kunishige Kataoka, Yukio Mukai, Tohru Yoshimura

Biochem J (2008) 409 (2): 399–406.

https://doi.org/10.1042/BJ20070642

Published: 21 December 2007

...Tomokazu Ito; Hisashi Hemmi; Kunishige Kataoka; Yukio Mukai; Tohru Yoshimura YGL196W of Saccharomyces cerevisiae encodes a putative protein that is unidentified but is predicted to have a motif similar to that of the N-terminal domain of the bacterial alanine racemase. In the present study we found...

View article titled, A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae

Includes: Supplementary data

Articles

SFH2 regulates fatty acid synthase activity in the yeast Saccharomyces cerevisiae and is critical to prevent saturated fatty acid accumulation in response to haem and oleic acid depletion

Thomas Desfougères, Thierry Ferreira, Thierry Bergès, Matthieu Régnacq

Biochem J (2008) 409 (1): 299–309.

https://doi.org/10.1042/BJ20071028

Published: 11 December 2007

...Thomas Desfougères; Thierry Ferreira; Thierry Bergès; Matthieu Régnacq The yeast Saccharomyces cerevisiae is a facultative anaerobic organism. Under anaerobiosis, sustained growth relies on the presence of exogenously supplied unsaturated fatty acids and ergosterol that yeast is unable...

View article titled, SFH2 regulates fatty acid synthase activity in the yeast Saccharomyces cerevisiae and is critical to prevent saturated fatty acid accumulation in response to haem and oleic acid depletion

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only