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ERK1/2 inhibitors act as monovalent degraders inducing ubiquitylation and proteasome-dependent turnover of ERK2, but not ERK1

In Collection

Cell signaling in the 21st century

Kathryn Balmanno, Andrew M. Kidger, Dominic P. Byrne, Matthew J. Sale, Nejma Nassman, Patrick A. Eyers, Simon J. Cook

Biochem J (2023) 480 (9): 587–605.

https://doi.org/10.1042/BCJ20220598

Published: 04 May 2023

... on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . ERK inhibitors extracellular signal-regulated kinases MEK RAF RAS ubiquitin proteasome system The RAS-regulated RAF-MEK1/2-ERK1/2 signalling pathway drives cell survival...

View article titled, ERK1/2 inhibitors act as monovalent degraders inducing ubiquitylation and proteasome-dependent turnover of ERK2, but not ERK1

Includes: Supplementary data

Articles

RAS Function in cancer cells: translating membrane biology and biochemistry into new therapeutics

Walaa E. Kattan, John F. Hancock

Biochem J (2020) 477 (15): 2893–2919.

https://doi.org/10.1042/BCJ20190839

Published: 14 August 2020

...Walaa E. Kattan; John F. Hancock The three human RAS proteins are mutated and constitutively activated in ∼20% of cancers leading to cell growth and proliferation. For the past three decades, many attempts have been made to inhibit these proteins with little success. Recently; however, multiple...

View article titled, RAS Function in cancer cells: translating membrane biology and biochemistry into new therapeutics

Articles

Amplification of PIP3 signalling by macropinocytic cups

Robert R. Kay, Thomas D. Williams, Peggy Paschke

Biochem J (2018) 475 (3): 643–648.

https://doi.org/10.1042/BCJ20170785

Published: 14 February 2018

...Robert R. Kay; Thomas D. Williams; Peggy Paschke In a role distinct from and perhaps more ancient than that in signal transduction, PIP3 and Ras help to spatially organize the actin cytoskeleton into macropinocytic cups. These large endocytic structures are extended by actin polymerization from...

View article titled, Amplification of PIP3 signalling by macropinocytic cups

Articles

An essential role for functional lysosomes in ferroptosis of cancer cells

Seiji Torii, Ryosuke Shintoku, Chisato Kubota, Makoto Yaegashi, Ryoko Torii, Masaya Sasaki, Toshinobu Suzuki, Masanobu Mori, Yuhei Yoshimoto, Toshiyuki Takeuchi, Keiichi Yamada

Biochem J (2016) 473 (6): 769–777.

https://doi.org/10.1042/BJ20150658

Published: 10 March 2016

...Seiji Torii; Ryosuke Shintoku; Chisato Kubota; Makoto Yaegashi; Ryoko Torii; Masaya Sasaki; Toshinobu Suzuki; Masanobu Mori; Yuhei Yoshimoto; Toshiyuki Takeuchi; Keiichi Yamada Pharmacological challenges to oncogenic Ras-expressing cancer cells have shown a novel type of cell death, ferroptosis...

View article titled, An essential role for functional lysosomes in ferroptosis of cancer cells

Includes: Supplementary data

Articles

A novel RCE1 isoform is required for H-Ras plasma membrane localization and is regulated by USP17

Jakub Jaworski, Ureshnie Govender, Cheryl McFarlane, Michelle de la Vega, Michelle K. Greene, Neil D. Rawlings, James A. Johnston, Christopher J. Scott, James F. Burrows

Biochem J (2014) 457 (2): 289–300.

https://doi.org/10.1042/BJ20131213

Published: 20 December 2013

...Jakub Jaworski; Ureshnie Govender; Cheryl McFarlane; Michelle de la Vega; Michelle K. Greene; Neil D. Rawlings; James A. Johnston; Christopher J. Scott; James F. Burrows Processing of the ‘CaaX’ motif found on the C-termini of many proteins, including the proto-oncogene Ras, requires the ER...

View article titled, A novel RCE1 isoform is required for H-Ras plasma membrane localization and is regulated by USP17

Includes: Supplementary data

Articles

Ras palmitoylation is necessary for N-Ras activation and signal propagation in growth factor signalling

Shu-Ping Song, Anne Hennig, Katja Schubert, Robby Markwart, Philipp Schmidt, Ian A. Prior, Frank-Dietmar Böhmer, Ignacio Rubio

Biochem J (2013) 454 (2): 323–332.

https://doi.org/10.1042/BJ20121799

Published: 09 August 2013

...Shu-Ping Song; Anne Hennig; Katja Schubert; Robby Markwart; Philipp Schmidt; Ian A. Prior; Frank-Dietmar Böhmer; Ignacio Rubio Ras GTPases undergo post-translational modifications that govern their subcellular trafficking and localization. In particular, palmitoylation of the Golgi tags N-Ras and H...

View article titled, Ras palmitoylation is necessary for N-Ras activation and signal propagation in growth factor signalling

Includes: Supplementary data

Articles

A requirement for wild-type Ras isoforms in mutant KRas-driven signalling and transformation

Carolyn Bentley, Stefanie S. Jurinka, Noelyn M. Kljavin, Steffan Vartanian, Sree R. Ramani, Lino C. Gonzalez, Kebing Yu, Zora Modrusan, Pan Du, Richard Bourgon, Richard M. Neve, David Stokoe

Biochem J (2013) 452 (2): 313–320.

https://doi.org/10.1042/BJ20121578

Published: 10 May 2013

..., but less is known about the role of WT (wild-type) Ras alleles and isoforms in cancer. We used zinc-finger nucleases targeting HRas and NRas to modify both alleles of these genes in the mutant KRas-driven Hec1A endometrial cancer cell line, which normally expresses WT copies of these genes. The disruption...

View article titled, A requirement for wild-type Ras isoforms in mutant KRas-driven signalling and transformation

Includes: Supplementary data

Articles

The BH3 mimetic ABT-263 synergizes with the MEK1/2 inhibitor selumetinib/AZD6244 to promote BIM-dependent tumour cell death and inhibit acquired resistance

Matthew J. Sale, Simon J. Cook

Biochem J (2013) 450 (2): 285–294.

https://doi.org/10.1042/BJ20121212

Published: 15 February 2013

... survival of colorectal cancer and melanoma cell lines with BRAF V600E or RAS mutations. This cell death absolutely required BAX (BCL2-associated X protein) and was inhibited by RNAi (RNA interference)-mediated knockdown of BIM (BCL2-interacting mediator of cell death) in the BRAF V600E -positive COLO205...

View article titled, The BH3 mimetic ABT-263 synergizes with the MEK1/2 inhibitor selumetinib/AZD6244 to promote BIM-dependent tumour cell death and inhibit acquired resistance

Includes: Supplementary data

Articles

Identification of the topology and functional domains of PAQR10

Ting Jin, Daqian Xu, Qiurong Ding, Yixuan Zhang, Chenqian Mao, Yi Pan, Zhenzhen Wang, Yan Chen

Biochem J (2012) 443 (3): 643–653.

https://doi.org/10.1042/BJ20112105

Published: 16 April 2012

...Ting Jin; Daqian Xu; Qiurong Ding; Yixuan Zhang; Chenqian Mao; Yi Pan; Zhenzhen Wang; Yan Chen PAQR10 (progestin and adipoQ receptor 10) is a Golgi-localized protein that is able to enhance the retention and activation of Ras proteins in the Golgi apparatus, subsequently leading to a sustained ERK...

View article titled, Identification of the topology and functional domains of PAQR10

Includes: Supplementary data

Articles

Phospholipase D1 mediates bFGF-induced Bcl-2 expression leading to neurite outgrowth in H19-7 cells

Sung Nyo Yoon, Kang Sik Kim, Ju Hwan Cho, Weina Ma, Hye-Jin Choi, Sung-Joon Kwon, Joong-Soo Han

Biochem J (2012) 441 (1): 407–416.

https://doi.org/10.1042/BJ20110302

Published: 14 December 2011

..., whereas PLD1 overexpression markedly induced Bcl-2 expression. bFGF treatment activated Ras, Src, PI3K (phosphoinositide 3-kinase), PLCγ (phospholipase Cγ) and PKCα (protein kinase Cα). Among these molecules, Src and PKCα were not required for Bcl-2 expression. PLD activity was decreased by Ras, PI3K...

View article titled, Phospholipase D1 mediates bFGF-induced Bcl-2 expression leading to neurite outgrowth in H19-7 cells

Articles

The progesterone receptor as a transcription factor regulates phospholipase D1 expression through independent activation of protein kinase A and Ras during 8-Br-cAMP-induced decidualization in human endometrial stromal cells

Ju Hwan Cho, Mee-Sup Yoon, Jun Bon Koo, Yong Seok Kim, Ki-Sung Lee, Jung Han Lee, Joong-Soo Han

Biochem J (2011) 436 (1): 181–191.

https://doi.org/10.1042/BJ20101614

Published: 27 April 2011

... decidualization of hES cells. In the present study, we focused on how PLD1 expression is up-regulated during decidualization. Treatment with PKA (protein kinase A) inhibitors (Rp-cAMP or H89) or a Ras inhibitor (manumycin) partially inhibited PLD1 expression and decidua formation in response to cAMP treatment...

View article titled, The progesterone receptor as a transcription factor regulates phospholipase D1 expression through independent activation of protein kinase A and Ras during 8-Br-cAMP-induced decidualization in human endometrial stromal cells

Includes: Supplementary data

Articles

The Golgi apparatus: an organelle with multiple complex functions

Cathal Wilson, Rossella Venditti, Laura R. Rega, Antonino Colanzi, Giovanni D'Angelo, M. Antonietta De Matteis

Biochem J (2011) 433 (1): 1–9.

https://doi.org/10.1042/BJ20101058

Published: 15 December 2010

... calcium homoeostasis Golgi complex mitotic entry Ras signalling Src The GC (Golgi complex) is a central organelle in the endomembrane system whose origin dates back to the last common eukaryotic ancestor [ 1 ]. Despite its ancient origin and the conservation of many of the Golgi-associated...

View article titled, The Golgi apparatus: an organelle with multiple complex functions

Includes: Supplementary data

Articles

Electrostatic interactions play a minor role in the binding of ExoS to 14-3-3 proteins

Lubna Yasmin, Jeffrey L. Veesenmeyer, Maureen H. Diaz, Matthew S. Francis, Christian Ottmann, Ruth H. Palmer, Alan R. Hauser, Bengt Hallberg

Biochem J (2010) 427 (2): 217–224.

https://doi.org/10.1042/BJ20100043

Published: 29 March 2010

... 2010 8 2 2010 9 2 2010 9 2 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 ADP-ribosyltransferase Akt/protein kinase B (PKB) exoenzyme S (ExoS) 14-3-3 protein Pseudomonas aeruginosa Ras The highly conserved eukaryotic protein family 14-3...

View article titled, Electrostatic interactions play a minor role in the binding of ExoS to 14-3-3 proteins

Includes: Supplementary data

Articles

Ras triggers acidosis-induced activation of the extracellular-signal-regulated kinase pathway in cardiac myocytes

Robert S. Haworth, Semjidmaa Dashnyam, Metin Avkiran

Biochem J (2006) 399 (3): 493–501.

https://doi.org/10.1042/BJ20051628

Published: 13 October 2006

... the phosphorylation of ERK1/2 by sustained intracellular acidosis, as did inhibition of Raf-1 with GW 5074 or ZM 336372. Interference with Ras signalling by the adenoviral expression of dominant-negative N17-Ras protein or with FPT III (farnesyl protein transferase inhibitor III) also prevented acidosis-induced ERK1...

View article titled, Ras triggers acidosis-induced activation of the extracellular-signal-regulated kinase pathway in cardiac myocytes

Articles

Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells

Ignacio Rubio, Knut Rennert, Ute Wittig, Katrin Beer, Matthias Dürst, Stacey L. Stang, Jim Stone, Reinhard Wetzker

Biochem J (2006) 398 (2): 243–256.

https://doi.org/10.1042/BJ20060160

Published: 15 August 2006

...Ignacio Rubio; Knut Rennert; Ute Wittig; Katrin Beer; Matthias Dürst; Stacey L. Stang; Jim Stone; Reinhard Wetzker Ras is a major mediator of PE (phorbol ester) effects in mammalian cells. Various mechanisms for PE activation of Ras have been reported [Downward, Graves, Warne, Rayter and Cantrell...

View article titled, Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells

Articles

Gα q binds to p110α/p85α phosphoinositide 3-kinase and displaces Ras

Lisa M. Ballou, Mohar Chattopadhyay, Yan Li, Suzanne Scarlata, Richard Z. Lin

Biochem J (2006) 394 (3): 557–562.

https://doi.org/10.1042/BJ20051493

Published: 24 February 2006

... showed that Gα q competes with Ras, a PI3K activator, for binding to p110α/p85α. Interestingly, co-precipitation studies using deletion mutants showed that Gα q binds to the p85-binding domain of p110α and not to the Ras-binding domain. Expression of constitutively active Gα q Q209L in cells inhibited...

View article titled, Gα q binds to p110α/p85α phosphoinositide 3-kinase and displaces Ras

Articles

Quantitative model of Ras–phosphoinositide 3-kinase signalling cross-talk based on co-operative molecular assembly

Harjeet Kaur, Chang Shin Park, Jodee M. Lewis, Jason M. Haugh

Biochem J (2006) 393 (1): 235–243.

https://doi.org/10.1042/BJ20051022

Published: 12 December 2005

...Harjeet Kaur; Chang Shin Park; Jodee M. Lewis; Jason M. Haugh In growth-factor-stimulated signal transduction, cell-surface receptors recruit PI3Ks (phosphoinositide 3-kinases) and Ras-specific GEFs (guanine nucleotide-exchange factors) to the plasma membrane, where they produce 3′-phosphorylated...

View article titled, Quantitative model of Ras–phosphoinositide 3-kinase signalling cross-talk based on co-operative molecular assembly

Includes: Supplementary data

Articles

Ras plasma membrane signalling platforms

John F. HANCOCK, Robert G. PARTON

Biochem J (2005) 389 (1): 1–11.

https://doi.org/10.1042/BJ20050231

Published: 21 June 2005

... will consider specifically how Ras proteins interact with the plasma membrane. The focus will be on recent studies that provide novel spatial and dynamic insights into the micro-environments that different Ras proteins utilize for signal transduction. We will correlate these recent studies suggesting Ras...

View article titled, Ras plasma membrane signalling platforms

Articles

Distinct requirements for the Sprouty domain for functional activity of Spred proteins

James A. J. KING, Andrew F. L. STRAFFON, Giovanna M. D'ABACO, Carole L. C. POON, Stacey T. T. I, Craig M. SMITH, Michael BUCHERT, Niall M. CORCORAN, Nathan E. HALL, Bernard A. CALLUS, Boris SARCEVIC, Daniel MARTIN, Peter LOCK, Christopher M. HOVENS

Biochem J (2005) 388 (2): 445–454.

https://doi.org/10.1042/BJ20041284

Published: 24 May 2005

... the Sprouty domain is required for Spred-2 function. We also demonstrate that the inhibitory function of Spred proteins is restricted to the Ras/MAPK pathway, that tyrosine phosphorylation is not required for this function, and that the Sprouty domain mediates heterodimer formation of Spred proteins. Growth...

View article titled, Distinct requirements for the Sprouty domain for functional activity of Spred proteins

Includes: Supplementary data

Articles

The effect of the farnesyl protein transferase inhibitor SCH66336 on isoprenylation and signalling by the prostacyclin receptor

Sarah J. O'MEARA, B. Therese KINSELLA

Biochem J (2005) 386 (1): 177–189.

https://doi.org/10.1042/BJ20041290

Published: 08 February 2005

...Sarah J. O'MEARA; B. Therese KINSELLA Like Ras, farnesylation of the IP (prostacyclin receptor) is required for its efficient intracellular signalling, and hence the IP represents a potential target for inhibition by FTIs [FTase (farnesyl protein transferase) inhibitors]. Herein, the effect...

View article titled, The effect of the farnesyl protein transferase inhibitor SCH66336 on isoprenylation and signalling by the prostacyclin receptor

Articles

RLP, a novel Ras-like protein, is an immediate-early transforming growth factor-β (TGF-β) target gene that negatively regulates transcriptional activity induced by TGF-β

Ester PIEK, Maarten van DINTHER, W. Tony PARKS, John M. SALLEE, Erwin P. BÖTTINGER, Anita B. ROBERTS, Peter ten DIJKE

Biochem J (2004) 383 (1): 187–199.

https://doi.org/10.1042/BJ20040774

Published: 24 September 2004

... in Smad2 or Smad3 [Yang, Piek, Zavadil, Liang, Xie, Heyer, Pavlidis, Kucherlapati, Roberts and Böttinger (2003) Proc. Natl. Acad. Sci. U.S.A. 100 , 10269–10274]. Among the TGF-β target genes identified, a novel gene with sequence homology to members of the Ras superfamily was identified, which we have...

View article titled, RLP, a novel Ras-like protein, is an immediate-early transforming growth factor-β (TGF-β) target gene that negatively regulates transcriptional activity induced by TGF-β

Articles

Regulation of the MST1 kinase by autophosphorylation, by the growth inhibitory proteins, RASSF1 and NORE1, and by Ras

Maria PRASKOVA, Andrei KHOKLATCHEV, Sara ORTIZ-VEGA, Joseph AVRUCH

Biochem J (2004) 381 (2): 453–462.

https://doi.org/10.1042/BJ20040025

Published: 06 July 2004

.... Endogenous MST1 immunoprecipitated from KB cells is specifically associated with substoichiometric amounts of the growth inhibitory polypeptides RASSF1A and NORE1A (novel Ras effector 1A; a Ras-GTP-binding protein). Co-expression of RASSF1A, RASSF1C, NORE1A and NORE1B with MST1 markedly suppresses MST1(Thr...

View article titled, Regulation of the MST1 kinase by autophosphorylation, by the growth inhibitory proteins, RASSF1 and NORE1, and by Ras

Articles

p120 Ras GTPase-activating protein associates with fibroblast growth factor receptors in Drosophila

Simon A. WOODCOCK, David A. HUGHES

Biochem J (2004) 380 (3): 767–774.

https://doi.org/10.1042/bj20031848

Published: 15 June 2004

...Simon A. WOODCOCK; David A. HUGHES Btl (breathless) and Htl (heartless), the two FGFRs (fibroblast growth factor receptors) in Drosophila melanogaster , control cell migration and differentiation in the developing embryo. These receptors signal through the conserved Ras/mitogen-activated protein...

View article titled, p120 Ras GTPase-activating protein associates with fibroblast growth factor receptors in Drosophila

Articles

Hormonal regulation of phospholipase Cepsilon through distinct and overlapping pathways involving G12 and Ras family G-proteins

Grant G. KELLEY, Sarah E. REKS, Alan V. SMRCKA

Biochem J (2004) 378 (1): 129–139.

https://doi.org/10.1042/bj20031370

Published: 15 February 2004

...Grant G. KELLEY; Sarah E. REKS; Alan V. SMRCKA PLC∊ (phospholipase C∊) is a novel PLC that has a CDC25 guanine nucleotide exchange factor domain and two RA (Ras-association) domains of which the second (RA2) is critical for Ras activation of the enzyme. In the present studies, we examined hormonal...

View article titled, Hormonal regulation of phospholipase Cepsilon through distinct and overlapping pathways involving G12 and Ras family G-proteins

Articles

Transcriptional and translational control of ornithine decarboxylase during Ras transformation

Lisa M. SHANTZ

Biochem J (2004) 377 (1): 257–264.

https://doi.org/10.1042/bj20030778

Published: 01 January 2004

...Lisa M. SHANTZ ODC (ornithine decarboxylase) activity is induced following ras activation. However, the Ras effector pathways responsible are unknown. These experiments used NIH-3T3 cells expressing partial-loss-of-function Ras mutants to activate selectively pathways downstream of Ras and examined...

View article titled, Transcriptional and translational control of ornithine decarboxylase during Ras transformation

Articles

Ras activation in response to lysophosphatidic acid requires a permissive input from the epidermal growth factor receptor

Ignacio RUBIO, Knut RENNERT, Ute WITTIG, Reinhard WETZKER

Biochem J (2003) 376 (3): 571–576.

https://doi.org/10.1042/bj20031410

Published: 15 December 2003

... signals at the level of Ras-GTP formation and Ras nucleotide exchange discriminates true mediatory signals from permissive activities that do not participate in signal relay. Hence, whereas pertussis toxin (PTX) treatment impairs stimulation of nucleotide exchange, epidermal growth factor receptor (EGFR...

View article titled, Ras activation in response to lysophosphatidic acid requires a permissive input from the epidermal growth factor receptor

Articles

Role of receptor tyrosine kinases in G-protein-coupled receptor regulation of Ras: transactivation or parallel pathways?

Julian DOWNWARD

Biochem J (2003) 376 (1): e9–e10.

https://doi.org/10.1042/bj20031745

Published: 15 November 2003

...Julian DOWNWARD Ras protein regulation by G-protein-coupled receptors has been thought to occur through transactivation of receptor tyrosine kinases. New evidence suggests that these two receptor types independently control different pathways leading to Ras activation in response...

View article titled, Role of receptor tyrosine kinases in G-protein-coupled receptor regulation of Ras: transactivation or parallel pathways?

Articles

Identification, functional expression and enzymic analysis of two distinct CaaX proteases from Caenorhabditis elegans

Juan CADIÑANOS, Walter K. SCHMIDT, Antonio FUEYO, Ignacio VARELA, Carlos LÓPEZ-OTÍN, José M.P. FREIJE

Biochem J (2003) 370 (3): 1047–1054.

https://doi.org/10.1042/bj20021514

Published: 15 March 2003

...Juan CADIÑANOS; Walter K. SCHMIDT; Antonio FUEYO; Ignacio VARELA; Carlos LÓPEZ-OTÍN; José M.P. FREIJE Post-translational processing of proteins such as the Ras GTPases, which contain a C-terminal CaaX motif (where C stands for cysteine, a for aliphatic and X is one of several amino acids), includes...

View article titled, Identification, functional expression and enzymic analysis of two distinct CaaX proteases from Caenorhabditis elegans

Articles

A novel form of the RelA nuclear factor κB subunit is induced by and forms a complex with the proto-oncogene c-Myc

Neil R. CHAPMAN, Gill A. WEBSTER, Peter J. GILLESPIE, Brian J. WILSON, Dorothy H. CROUCH, Neil D. PERKINS

Biochem J (2002) 366 (2): 459–469.

https://doi.org/10.1042/bj20020444

Published: 01 September 2002

... be addressed (e-mail n.d.perkins@dundee.ac.uk ). 22 3 2002 22 5 2002 23 5 2002 23 5 2002 The Biochemical Society, London ©2002 2002 cancer proteolysis Ras repression transcription Abbreviations used: DTT, dithiothreitol; EMSA, electrophoretic mobility-shift assay...

View article titled, A novel form of the RelA nuclear factor κB subunit is induced by and forms a complex with the proto-oncogene c-Myc

Articles

A novel role for farnesyl pyrophosphate synthase in fibroblast growth factor-mediated signal transduction

John F. REILLY, Shawndra D. MARTINEZ, Gregory MICKEY, Pamela A. MAHER

Biochem J (2002) 366 (2): 501–510.

https://doi.org/10.1042/bj20020560

Published: 01 September 2002

... proliferation, accompanied by a failure of the FGF-2-mediated induction of cyclins D1 and E. Overexpression of FPPS in fibroblasts also promotes increased farnesylation of Ras, and temporally extends FGF-2-stimulated activation of the Ras/ERK (extracellular-signal-regulated kinase) cascade. These data suggest...

View article titled, A novel role for farnesyl pyrophosphate synthase in fibroblast growth factor-mediated signal transduction

Articles

Regulation of phospholipase D isoenzymes by transforming Ras and atypical protein kinase C-ι

James MWANJEWE, Martin SPITALER, Maria EBNER, Michaela WINDEGGER, Markus GEIGER, Sonja KAMPFER, Johann HOFMANN, Florian ÜBERALL, Hans H. GRUNICKE

Biochem J (2001) 359 (1): 211–217.

https://doi.org/10.1042/bj3590211

Published: 24 September 2001

...James MWANJEWE; Martin SPITALER; Maria EBNER; Michaela WINDEGGER; Markus GEIGER; Sonja KAMPFER; Johann HOFMANN; Florian ÜBERALL; Hans H. GRUNICKE The activation of phospholipase D (PLD) by transforming Ras is well documented. Although two distinct PLD isoforms, PLD1 and PLD2, have been cloned from...

View article titled, Regulation of phospholipase D isoenzymes by transforming Ras and atypical protein kinase C-ι

Articles

Phosphate-binding loop and Rab GTPase function: mutations at Ser29 and Ala30 of Rab5 lead to loss-of-function as well as gain-of-function phenotype

Guangpu LI, Zhimin LIANG

Biochem J (2001) 355 (3): 681–689.

https://doi.org/10.1042/bj3550681

Published: 24 April 2001

...Guangpu LI; Zhimin LIANG Ras-like GTPases contain a structurally conserved GTP-binding domain. An important element of the GTP-binding domain is the phosphate-binding loop, which contains two Gly residues (Gly 12 and Gly 13 ) in Ras. Because the two Gly residues are crucial for normal Ras function...

View article titled, Phosphate-binding loop and Rab GTPase function: mutations at Ser29 and Ala30 of Rab5 lead to loss-of-function as well as gain-of-function phenotype

Articles

Molecular modelling and site-directed mutagenesis of the inositol 1,3,4,5-tetrakisphosphate-binding pleckstrin homology domain from the Ras GTPase-activating protein GAP1IP4BP

Gyles COZIER, Richard SESSIONS, Joanna R. BOTTOMLEY, Jon S. REYNOLDS, Peter J. CULLEN

Biochem J (2000) 349 (1): 333–342.

https://doi.org/10.1042/bj3490333

Published: 26 June 2000

...Gyles COZIER; Richard SESSIONS; Joanna R. BOTTOMLEY; Jon S. REYNOLDS; Peter J. CULLEN GAP1 IP4BP is a Ras GTPase-activating protein (GAP) that in vitro is regulated by the cytosolic second messenger inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P 4 ]. We have studied Ins(1,3,4,5)P 4 binding...

View article titled, Molecular modelling and site-directed mutagenesis of the inositol 1,3,4,5-tetrakisphosphate-binding pleckstrin homology domain from the Ras GTPase-activating protein GAP1IP4BP

Articles

Specific role of the extracellular signal-regulated kinase pathway in angiotensin II-induced cardiac hypertrophy in vitro

Hiroki AOKI, Mary RICHMOND, Seigo IZUMO, Junichi SADOSHIMA

Biochem J (2000) 347 (1): 275–284.

https://doi.org/10.1042/bj3470275

Published: 27 March 2000

... promoter activation was significantly inhibited by SB203580 and dominant-negative MEKK1, but not by PD98059 or dominant-negative MEK1. Dominant-negative Ras inhibited both ERK activation and ANF up-regulation by Ang II, whereas constitutively active forms of Ras and MEK were sufficient to activate the ANF...

View article titled, Specific role of the extracellular signal-regulated kinase pathway in angiotensin II-induced cardiac hypertrophy in vitro

Articles

GTPase mechanism and function: new insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5

Zhimin LIANG, Timothy MATHER, Guangpu LI

Biochem J (2000) 346 (2): 501–508.

https://doi.org/10.1042/bj3460501

Published: 22 February 2000

...Zhimin LIANG; Timothy MATHER; Guangpu LI Structural and biochemical data indicate the importance of the phosphate-binding loop residues Gly 12 and Gly 13 of Ras both in the GTP hydrolysis reaction and in biological activity, but these two residues are not conserved in other Ras-related GTPases...

View article titled, GTPase mechanism and function: new insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5

Articles

Sphingosine 1-phosphate stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle

Soma RAKHIT, Ann-Marie CONWAY, Rothwelle TATE, Tara BOWER, Nigel J. PYNE, Susan PYNE

Biochem J (1999) 338 (3): 643–649.

https://doi.org/10.1042/bj3380643

Published: 08 March 1999

... , n.j.pyne@strath.ac.uk ). 18 8 1998 27 10 1998 5 1 1999 The Biochemical Society, London © 1999 1999 lipid kinases proliferation Ras sphingolipids Biochem. J. (1999) 338, 643 649 (Printed in Great Britain) 643 Sphingosine 1-phosphate stimulation of the p42/p44 mitogen...

View article titled, Sphingosine 1-phosphate stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle

Articles

Synergistic activation of JNK/SAPK by interleukin-1 and platelet-derived growth factor is independent of Rac and Cdc42

W. DAVIS, Leonard R. STEPHENS, Phillip T. HAWKINS, Jeremy SAKLATVALA

Biochem J (1999) 338 (2): 387–392.

https://doi.org/10.1042/bj3380387

Published: 22 February 1999

... activation of JNK by interleukin 1 (IL-1) in human fibroblasts and a pig aortic endothelial (PAE) cell line. This synergistic activation of JNK by IL-1 and PDGF was unaffected by bacterial toxins that inactivate Rho proteins and Ras. Since Rho proteins have been implicated in JNK activation, their possible...

View article titled, Synergistic activation of JNK/SAPK by interleukin-1 and platelet-derived growth factor is independent of Rac and Cdc42

Articles

Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor

Jee-Young KIM, Myung-Soon YANG, Chun-Do OH, Kyong-Tai KIM, Mahn Joon HA, Shin-Sung KANG, Jang-Soo CHUN

Biochem J (1999) 337 (2): 275–280.

https://doi.org/10.1042/bj3370275

Published: 08 January 1999

... Erk-1/-2 activation. Ectopic expression of inhibitory small GTPase Ras, RasN17, blocked the carbachol-induced Raf activation without affecting the activation of PKCε, while the inhibition of PKC blocked the Raf activation. Thus, these results suggest that carbachol-induced activation of PKCε mediates...

View article titled, Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor

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Platelet-derived-growth-factor stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle: role of pertussis-toxin-sensitive G-proteins, c-Src tyrosine kinases and phosphoinositide 3-kinase

Ann-Marie CONWAY, Soma RAKHIT, Susan PYNE, Nigel J. PYNE

Biochem J (1999) 337 (2): 171–177.

https://doi.org/10.1042/bj3370171

Published: 08 January 1999

... ). 20 4 1998 2 9 1998 15 10 1998 The Biochemical Society, London © 1999 1999 growth-factor receptor lipid kinase mitogenesis protein kinase Ras Biochem. J. (1999) 337, 171 177 (Printed in Great Britain) 171 Platelet-derived-growth-factor stimulation of the p42/p44 mitogen...

View article titled, Platelet-derived-growth-factor stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle: role of pertussis-toxin-sensitive G-proteins, c-Src tyrosine kinases and phosphoinositide 3-kinase

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