... by regulating the expression of the dormancy regulon. We have previously shown that the interaction of DevR with RNA polymerase is essential for the expression of DevR-regulated genes. Here, we developed a M. tuberculosis -specific in vivo transcription system to enrich our understanding of DevR–RNA polymerase...

... with the RNA polymerase core enzyme (E) to form holoenzymes (Eσ) with different promoter recognition specificities. The alternative σ factor RpoS/σ S is produced in stationary phase and under stress conditions and reprograms global gene expression to promote bacterial survival. To date, the three-dimensional...

...Xiaojuan Zhang; Zhixuan Yao; Yanting Duan; Xiaomei Zhang; Jinsong Shi; Zhenghong Xu The specific recognition and binding of promoter and RNA polymerase is the first step of transcription initiation in bacteria and largely determines transcription activity. Therefore, direct analysis...

...Ivan Petushkov; Daria Esyunina; Vladimir Mekler; Konstantin Severinov; Danil Pupov; Andrey Kulbachinskiy In bacterial RNA polymerase (RNAP), conserved region 3.2 of the σ subunit was proposed to contribute to promoter escape by interacting with the 5′-end of nascent RNA, thus facilitating σ...

... regulation of alternative splicing. The epigenetic modifications may regulate alternative splicing by either influencing the transcription elongation rate of RNA polymerase II or by recruiting a specific splicing regulator via different chromatin adaptors. The epigenetic alterations and aberrant alternative...

...Aleksei Agapov; Daria Esyunina; Danil Pupov; Andrey Kulbachinskiy Transcription factors of the Gre family bind within the secondary channel of bacterial RNA polymerase (RNAP) directly modulating its catalytic activities. Universally conserved Gre factors activate RNA cleavage by RNAP, by chelating...

... through its ability to lock the RNA polymerase down into a state unable to melt out promoter DNA for transcription initiation. Promoter DNA opening then occurs through the action of specialized transcription control proteins called bacterial enhancer-binding proteins (bEBPs) that remodel the sigma54...

...Paola Cavaliere; Fabienne Levi-Acobas; Claudine Mayer; Frederick A. Saul; Patrick England; Patrick Weber; Bertrand Raynal; Véronique Monteil; Jacques Bellalou; Ahmed Haouz; Françoise Norel In many γ-proteobacteria, the RpoS/σ S sigma factor associates with the core RNAP (RNA polymerase) to modify...

...Je Ko; Tomasz Heyduk Promoter escape by RNA polymerase, the transition between the initiation and elongation, is a critical step that defines transcription output at many promoters. In the present study we used a real-time fluorescence assay for promoter melting and escape to study the determinants...

... the optimal promoter spacing. Moreover, shortening in length of the flexible linker between the two promoter DNA-binding domains of σ A also does not enable SND100-σ A to sense the optimal promoter spacing. Efficient recognition of optimal promoter spacing by SND100-σ A requires core RNAP (RNA polymerase...

...Danil Pupov; Daria Esyunina; Andrey Feklistov; Andrey Kulbachinskiy Besides canonical double-strand DNA promoters, multisubunit RNAPs (RNA polymerases) recognize a number of specific single-strand DNA and RNA templates, resulting in synthesis of various types of RNA transcripts. The general...

...Dina Grohmann; Angela Hirtreiter; Finn Werner Archaeal and eukaryotic RNAPs (DNA-dependent RNA polymerases) are complex multi-subunit enzymes. Two of the subunits, F and E, which together form the F/E complex, have been hypothesized to associate with RNAP in a reversible manner during...

...Boon Shang Chew; Norbert Lehming The TBP (TATA-box-binding protein), Tbp1p, plays a vital role in all three classes of transcription by RNA polymerases I–III. A TBP1 (E186D) mutation had been described that affected interaction of Tbp1p with TFIIB (transcription factor IIB) and that caused slow...