... to environmental stimuli in both prokaryotic and eukaryotic organisms, these types of proteins are still uncharacterized in the trypanosomatid parasites, Trypanosome and Leishmania . In addition, PAS-containing phosphoglycerate kinase (PGK) protein is uncharacterized in the literature. Here, we report a PAS domain...

... the effectiveness of this approach against trypanosomatid parasites [ 4 ]. PFKs from the three trypanosomatid species that cause significant mortality and morbidity: Trypanosoma brucei (Human African Trypanosomiasis), Trypanosoma cruzi (Chagas disease) and Leishmania spp . (Leishmaniasis) are all potential...

...Rubén Perandrés-López; María P. Sánchez-Cañete; Francisco Gamarro; Santiago Castanys The P4 family of P-type ATPases (P4-ATPases) plays an important role in maintaining phospholipid asymmetry in eukaryotic cell membranes. Leishmania miltefosine transporter (LMT) is a plasma membrane (PM) P4-ATPase...

...Normand Cyr; Terry K. Smith; Élodie Boisselier; Louis-Philippe Leroux; Anwar Hasil Kottarampatel; Amanda Davidsen; Christian Salesse; Armando Jardim Protein import into the Leishmania glycosome requires docking of the cargo-loaded peroxin 5 (PEX5) receptor to the peroxin 14 (PEX14) bound...

...Ana Perea; José Ignacio Manzano; Yasuhisa Kimura; Kazumitsu Ueda; Santiago Castanys; Francisco Gamarro The Leishmania LABCG2 transporter has a key role in the redox metabolism of these protozoan parasites. Recently, the involvement of LABCG2 in virulence, autophagy and oxidative stress has been...

...Hamed Hojjat; Armando Jardim Trafficking of peroxisomal targeting signal 1 (PTS1) proteins to the Leishmania glycosome is dependent on the docking of the LdPEX5 receptor to LdPEX14 on the glycosomal membrane. A combination of deletion and random mutagenesis was used to identify residues...

...Ana Victoria C. Pilar; Rona Strasser; James McLean; Elizabeth Quinn; Normand Cyr; Hamed Hojjat; Anwer Hasil Kottarampatel; Armando Jardim LPEX7 ( Leishmania peroxin 7) is essential for targeting newly synthesized proteins with a PTS2 (peroxisome-targeting signal type 2) import signal...

... of P4-ATPases and proper translocase activity. In the present study, we analysed the role of Leishmania infantum LiRos3, the Cdc50 subunit of the P4-ATPase MLF (miltefosine) transporter [LiMT ( L. infantum MLF transporter)], on trafficking and complex functionality using site-directed mutagenesis...

... as reservoirs of organic osmolytes. These reflect adaptation to their insect vectors whose haemolymphs are exceptionally rich in the two amino acids. In the present study we identify and characterize a new neutral amino acid transporter, LdAAP24, that translocates proline and alanine across the Leishmania...

... Attribution Non-Commercial Licence ( http://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. α-difluoromethylornithine Leishmania malaria ornithine decarboxylase (ODC...

..., in contrast with its mammalian counterpart which is predominately nucleoplasmic, is localized at the nuclear rim. The aim of the present study was to characterize the Ld Ran ( Leishmania donovani Ran) orthologue with an emphasis on the Ran–histone association. Ld Ran was found to be developmentally regulated...

...Roderick A. M. Williams; Gareth D. Westrop; Graham H. Coombs Genome mining and biochemical analyses have shown that Leishmania major possesses two pathways for cysteine synthesis – the de novo biosynthesis pathway comprising SAT (serine acetyltransferase) and CS (cysteine synthase) and the RTS...

... of inhibitors against this target. Tb TDPX2 from strain S427 was cloned into pET15b (Novagen), expressed in Escherichia coli BL21(DE3)pLysS and purified as the His 6 -tagged protein essentially as described for Lm TDPX1 ( Leishmania major TDPX1) [ 13 ]. The His 6 tag was removed...

...Somdeb Bosedasgupta; Benu Brata Das; Souvik Sengupta; Agneyo Ganguly; Amit Roy; Gayatri Tripathi; Hemanta K. Majumder The unusual, heterodimeric topoisomerase IB of Leishmania shows functional activity upon reconstitution of the DNA-binding large subunit (LdTOPIL; or L) and the catalytic small...

...Kleber P. Madrid; Armando Jardim Import of proteins with a PTS1 (peroxisomal targeting signal 1) into the Leishmania glycosomal organelle involves docking of a PTS1-laden LdPEX5 [ Leishmania donovani PEX5 (peroxin 5)] receptor to LdPEX14 on the surface of the glycosomal membrane. In higher...

...Tanushri Sengupta; Mandira Mukherjee; Aditi Das; Chhabinath Mandal; Rakhee Das; Tanmoy Mukherjee; Hemanta K. Majumder We have cloned and expressed the 43 kDa N-terminal domain of Leishmania donovani topoisomerase II. This protein has an intrinsic ATPase activity and obeys Michaelis–Menten kinetics...

...Helen DENTON; Joanne C. McGREGOR; Graham H. COOMBS The reason why Leishmania parasites are susceptible to organic antimonial drugs, the standard chemotherapeutic agents for over 50 years, apparently lies in the fact that the mammalian stage of the parasite reduces the pentavalent form...

...Manuel SOTO; Salvador IBORRA; Luis QUIJADA; Cristina FOLGUEIRA; Carlos ALONSO; Jose M. REQUENA The cell-cycle-dependent expression of the four core histones (H2A, H2B, H3 and H4) has been studied in the protozoan parasite Leishmania infantum . For that purpose, the cell cycle was arrested...

...J. Román LUQUE-ORTEGA; José M. SAUGAR; Cristina CHIVA; David ANDREU; Luis RIVAS Leishmanicidal drugs interacting stoichiometrically with parasite plasma membrane lipids, thus promoting permeability, have raised significant expectations for Leishmania chemotherapy due to their nil or very low...

...Sanjay GHOSH; Srikanta GOSWAMI; Samit ADHYA Intracellular parasitic protozoans of the genus Leishmania depend for their survival on the elaboration of enzymic and other mechanisms for evading toxic free-radical damage inflicted by their phagocytic macrophage host. One such mechanism may involve...

.... Size fractionation of the high-pH extract indicated that the active component(s) was 30–50kDa in size and was inactivated by iodoacetamide. Activity could also be restored by reconstituting the inactivated membranes with Escherichia coli -expressed, polyhistidine-tagged Leishmania mexicana GPI8 (GPI8...