... PtdIns5 P using conventional HPLC, owing to poor separation from PtdIns4 P . In the present paper we describe a new HPLC method for resolving PtdIns5 P from PtdIns4 P , which makes possible accurate measurements of basal and inducible levels of cellular PtdIns5 P in the context of other phosphoinositides...

... in the lumen of the ER. There is a growing amount of evidence that N-linked glycans play important roles in folding, and intra- and/or inter-cellular trafficking of N-glycosylated proteins [ 1 – 3 ]. cancer cells endoplasmic reticulum-associated degradation (ERAD) free complex-type glycans HPLC...

... ADPR are sufficient for TRPM2 gating in neutrophil granulocytes, we devised an HPLC method to determine ADPR contents in HClO 4 cell extracts. The reversed-phase ion-pair HPLC method with an Mg 2+ -containing isocratic eluent allows baseline resolution of one ADPR peak. Intracellular ADPR...

...- centration range from 0 23 to 2 mM. Key words: amino acids, biosynthetic pathway, HPLC, in Šitro model, pathway flux. EXPERIMENTAL Chemicals Aspartate, homoserine and threonine were from Sigma. d, l-Aspartic b-semialdehyde (ASA), O-phospho-homoserine and b-aspartyl phosphate (aspp) were synthesized by using...

.... Struct. Biol. 7 , 147Ő153], and (2) computer-modelling analyses of enzymeŐsubstrate complexes, a novel mode of phytic acid hydrolysis is proposed. HPLC metal-dye detection phytic acid Biochem. J. (2000) 352, 623 628 (Printed in Great Britain) 623 Analysis of myo-inositol hexakisphosphate...

... are absorbed by humans, as indicated by their appearance in the circulating blood. 25 9 1998 20 1 1999 5 2 1999 The Biochemical Society, London © 1999 1999 5,6-epoxy-b-carotene epoxyretinyl palmitate HPLC mango plasma Biochem. J. (1999) 339, 359 362 (Printed in Great Britain...

... by lack of exogenous substrate. This involved isolation of the acid-insoluble fraction of PAEC, which was presumed to contain purinogen, and analysis by HPLC and 31 P NMR. No evidence supporting the presence of triphosphate-containing compounds (purinogen) was found. Similar results were obtained...