... The Authors Journal compilation © 2014 Biochemical Society 2014 aequorin Ca 2+ measurement endoplasmic reticulum fluorescent Ca 2+ indicator GCaMP mitochondrion Since the identification of the intense Ca 2+ connection that occurs between the ER (endoplasmic reticulum) and mitochondria...

...+ indicators, such as the GCaMP series based on GFP, are powerful tools for imaging changes in the concentration of Ca 2+ associated with intracellular signalling pathways. Most GCaMP-type indicators have dissociation constants ( K d ) for Ca 2+ in the high nanomolar to low micromolar range and are therefore...