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Keywords: DNA binding
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Biochem J (2020) 477 (7): 1345–1362.
Published: 17 April 2020
... reinforced the proposed similarity to Fis and supported the identification of residues involved in DNA binding which were demonstrated experimentally. Overexpression of NinH proved toxic and this correlated with its capacity to associate with DNA. NinH is the first example of a phage-encoded Fis-like NAP...
Includes: Supplementary data
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Biochem J (2019) 476 (21): 3261–3279.
Published: 11 November 2019
... the DNA binding activity of HpDnaB. Furthermore, overexpression of Hp0897 full-length protein in H. pylori leads to an elongated cell phenotype. While the overexpression of Hp0897CTD does not show a phenotype of cell elongation, overexpression of Hp0897NTD shows extensive cell elongation. These results...
Includes: Supplementary data
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Biochem J (2013) 456 (2): 275–282.
Published: 08 November 2013
... extensions that modulate DNA-binding properties. We report in the present paper that the lysine-rich C-terminal extension of M. smegmatis Ku contacts the core protein domain as evidenced by an increase in DNA-binding affinity and a decrease in thermal stability and intrinsic tryptophan fluorescence upon its...
Includes: Supplementary data
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Biochem J (2013) 449 (3): 707–717.
Published: 09 January 2013
... cognate) 70-interacting protein] and MDM2 (murine double minute 2), which dock to the Mf2 domain, was specific for lysine residues found predominantly in loop structures that extend from the DNA-binding domain, whereas no modification was detected in the more conformationally flexible C-terminal half...
Includes: Supplementary data
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Biochem J (2012) 442 (3): 527–538.
Published: 24 February 2012
... Biochemical Society 2012 DNA binding histone methylation nucleosome Pdp1 PWWP domain Schizosaccharomyces pombe Set9 Post-translational modification of histones has emerged as a key mechanism for controlling chromosome processes, such as transcription. These modifications, including...
Includes: Supplementary data
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Biochem J (2005) 388 (3): 921–928.
Published: 07 June 2005
... metalloproteinase-12 (metalloelastase). YB-1 transrepression of both the minimal and matrix metalloproteinase-12 promoter reporter constructs is dependent on the AP-1 sequence. To identify new nuclear proteins that bind specifically to the AP-1 DNA-binding site, we devised a DNA-affinity-chromatography-based assay...
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Biochem J (2002) 362 (1): 41–50.
Published: 08 February 2002
... membrane cardiovascular DNA binding extracellular matrix transcription Abbreviations used: AP-2, activator protein 2; EMSA, electrophoretic mobility-shift assay. Biochem. J. (2002) 362, 41 50 (Printed in Great Britain) 41 Structural and functional characterization of the human and mouse...
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Biochem J (2002) 361 (1): 87–95.
Published: 17 December 2001
... to form oligomeric structures and that the presence of physiological ligands, such as nucleotides or heteroduplex DNA, but not homoduplex DNA, plays an important role in keeping the protein in an active conformation by preventing protein oligo- merization. Key words: DNA binding, electron microscopy, MutS...
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Biochem J (2000) 347 (3): 881–886.
Published: 25 April 2000
... aquaticus ( Taq ) MutS protein in solution. Analytical gel filtration and cross-linking of MutS protein with disuccinimidyl suburate suggest that Taq MutS is a dimer in the presence of ATP. ATP binding and hydrolysis by Taq MutS reduces the heteroduplex-DNA binding by the protein. Using limited proteolysis...
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Biochem J (2000) 345 (3): 417–421.
Published: 25 January 2000
... into the nucleus. We provide evidence that the event of STAT activation is more complex than previously assumed. Key words: DNA binding, HepG2 hepatoma cells, interleukin- 6, A375 melanoma cells, nuclear translocation. Here we show that STATs form stable complexes in hepatoma and melanoma cells before stimulation...
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Biochem J (1999) 344 (1): 85–92.
Published: 08 November 1999
... as a model for a universal inhibitor of the superfamily of DNA polymerases. 1 To whom correspondence should be addressed (e-mail: ahizy@post.tau.ac.il). 28 5 1999 3 8 1999 31 8 1999 The Biochemical Society, London © 1999 1999 DNA binding polycitrin A reverse...
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Biochem J (1999) 341 (1): 89–93.
Published: 24 June 1999
... 2 1999 12 3 1999 20 4 1999 The Biochemical Society, London © 1999 1999 DNA binding human mutation induced fit Pax genes protein structure Biochem. J. (1999) 341, 89 93 (Printed in Great Britain) 89 Structural defects of a Pax8 mutant that give rise to congenital...
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Biochem J (1999) 337 (2): 253–262.
Published: 08 January 1999
... accounting for a sort of chronology of events during DNA binding. Since the RED subdomain is much more variable than the PAI subdomain among Pax proteins, these results could explain how distinct Pax proteins may select different target genes. Biochem. J. (1999) 337, 253 262 (Printed in Great Britain) 253 Co...