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Keywords: Arabidopsis thaliana
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Biochem J (2023) 480 (8): 495–520.
Published: 21 April 2023
... that leaves of Arabidopsis thaliana contain a family of Prens composed of 9 to 11 isoprene units (Pren-9 to Pren-11) synthesized by CPT7. The Dol spectrum in A. thaliana (studied in hairy roots) comprises two main families: medium-length Dols (Dol-15, -16 and -17, synthesized by CPT3 [ 12 ]) and longer...
Includes: Supplementary data
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Biochem J (2020) 477 (24): 4785–4796.
Published: 24 December 2020
... 2020 arabidopsis thaliana crystallography protein structure tetrapyrroles Heme is an essential pigment for plants and participates in various key biological processes [ 1 ]. It carries electrons for the respiratory and photosynthetic electron transfer chains within mitochondria...
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Biochem J (2020) 477 (19): 3885–3896.
Published: 12 October 2020
... of Rubisco, we purified Rubisco from leaves of Arabidopsis thaliana in the daytime and night-time and established Lys-acetylation stoichiometries by two independent methods. Our findings indicate that Rubisco is Lys-acetylated at very low stoichiometries that could not meaningfully influence Rubisco...
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Biochem J (2020) 477 (19): 3743–3767.
Published: 12 October 2020
...) . Arabidopsis thaliana embryogenesis seed biology somatic embryo transcription factor Seeds first appeared a little under four million years ago [ 1 ]. Fossil evidence places the origin of angiosperms (flowering plants) during the Cretaceous, but molecular evidence supports an earlier origin [ 2...
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Biochem J (2020) 477 (16): 3019–3032.
Published: 26 August 2020
... in Arabidopsis thaliana by determining the abundance of different proteins during enrichment of ribosomes from cell cultures using peptide mass spectrometry. The turnover rates of 26 40S subunit r-proteins and 29 60S subunit r-proteins were also determined, showing that half of the ribosome population...
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Biochem J (2019) 476 (12): 1781–1790.
Published: 26 June 2019
... evidence that the TrxL2/2CP redox cascade supports oxidative activation of G6PDH. The direct involvement of H 2 O 2 in G6PDH activation is also shown. 2-Cys peroxiredoxin Arabidopsis thaliana glucose-6-phosphate dehydrogenase hydrogen peroxide redox regulation thioredoxin Total RNA...
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In Collection
Seeds
Biochem J (2019) 476 (6): 965–974.
Published: 22 March 2019
... by Portland Press Limited on behalf of the Biochemical Society 2019 abscisic acid Arabidopsis thaliana glutathione reactive oxygen species seed longevity vigour Seeds age during storage and the resulting loss of seed vigour and viability can impact crop yields and sustainable agricultural...
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In Collection
Seeds
Biochem J (2019) 476 (5): 843–857.
Published: 12 March 2019
... protein profiling of proteases to evaluate the quality of artificially and naturally aged seeds of Arabidopsis thaliana . Using this approach, we have identified two protease activities with opposite behaviours in aged seeds of Arabidopsis that correlate with the quality status of the seeds. We show...
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Biochem J (2019) 476 (5): 783–794.
Published: 06 March 2019
... in Arabidopsis thaliana , in complex with the ALA-interacting subunit 5 (ALIS5). The ATP hydrolytic activity of the ALA2–ALIS5 complex was stimulated in a highly specific manner by phosphatidylserine. Small changes in the stereochemistry or the functional groups of the phosphatidylserine head group affected...
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Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
... the enzyme activity of Arabidopsis thaliana LACS9 (AtLACS9) by introducing random mutations into its cDNA using error-prone PCR. Two AtLACS9 variants containing multiple amino acid residue substitutions were identified with enhanced enzyme activity. To explore the effect of each amino acid residue...
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Biochem J (2018) 475 (1): 61–74.
Published: 02 January 2018
... of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Arabidopsis thaliana AUX/IAA proteins protein modification SUMO SUMO chains We have previoulsy described a mutation in the first alpha helix of SCE, a Lys 15-to-Arg (K15R) change...
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Biochem J (2017) 474 (21): 3675–3687.
Published: 23 October 2017
... and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Arabidopsis thaliana eukaryotic gene expression myb transcription factor phosphate surface plasmon resonance Phosphorus (P) is well recognized for its importance in multiple biological processes; however, its...
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Biochem J (2017) 474 (11): 1789–1801.
Published: 10 May 2017
...John W. Riggs; Judy Callis The Arabidopsis thaliana fructokinase-like proteins FLN1 and FLN2 are required for the differentiation of plastids into photosynthetically competent chloroplasts. However, their specific roles are unknown. FLN1 and FLN2 localize in a multisubunit prokaryotic-type...
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Biochem J (2017) 474 (8): 1347–1360.
Published: 04 April 2017
..., Arabidopsis thaliana genome sequencing has revealed that as many as five Trx subtypes encoded by a total of 10 nuclear genes are targeted to chloroplasts. Because each Trx isoform seems to have a distinct target selectivity, the electron distribution from FTR to multiple Trxs is thought to be the critical...
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Biochem J (2016) 473 (11): 1605–1615.
Published: 27 May 2016
... Arabidopsis thaliana Cdc50 ectodomain disulfide bond flippase N-glycosylation The P-type ATPase superfamily comprises a large number of primary transmembrane-spanning transporters that share the common characteristic of forming a phosphorylated intermediate during their catalytic cycle [ 1 ]. P...
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Biochem J (2016) 473 (5): 593–603.
Published: 24 February 2016
... synthase isoforms are found in Arabidopsis thaliana each with specific substrate preferences and sensitivity to inhibitors and activators. Arabidopsis thaliana ceramide enzyme kinetics plant biochemistry sphingolipid Sphingolipids are a unique subset of membrane lipids...
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Biochem J (2016) 473 (2): 157–166.
Published: 05 January 2016
... to require an active ThMPase for growth. In vitro assays confirmed that these candidates all preferred ThMP to any of 45 other phosphate ester substrates tested. An Arabidopsis thaliana ThMPase homologue (At4g29530) of unknown function whose expression pattern and compartmentation fit with a role in ThDP...
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Biochem J (2015) 466 (1): 137–145.
Published: 06 February 2015
... in Arabidopsis thaliana and Escherichia coli helps maintain flavin levels. COG3236 proteins thus illustrate two emerging principles in chemical biology: directed overflow metabolism, in which excess flux is diverted out of a pathway, and the pre-emption of damage from reactive metabolites. Subsequent steps...
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Biochem J (2014) 459 (2): 289–299.
Published: 28 March 2014
... the Arabidopsis thaliana cpi gene were screened for inactive CPI mutant enzymes on the basis of their ability to genetically complement a Saccharomyces cerevisiae erg7 (defective in oxidosqualene cyclase) ergosterol auxotroph grown in the presence of exogenous cycloeucalenol, and led to the identification of four...
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Biochem J (2012) 448 (2): 243–251.
Published: 07 November 2012
... (email thorsten.seidel@uni-bielefeld.de or karin.schumacher@cos.uni-heidelberg.de ). 15 6 2012 28 8 2012 4 9 2012 4 9 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 Arabidopsis thaliana glutathione nitrosoglutathione (GSNO) redox...
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Biochem J (2012) 447 (2): 291–299.
Published: 26 September 2012
... 480 real-time PCR system using SYBR Green I master mix (Roche). TUB4 (tubulin β-chain 4; At5g44340) and EIF4a (eukaryotic translation initiation factor 4A; At3g13920) were used as control genes. All primers used for qRT-PCR have been described previously [ 24 ]. Arabidopsis thaliana...
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Biochem J (2011) 435 (1): 167–174.
Published: 15 March 2011
... unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Arabidopsis thaliana complex formation degenerated protease domain DEG7 serine protease taxonomic distribution Deg/HtrA (for deg radation of periplasmic proteins/ h...
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Biochem J (2010) 425 (1): 207–218.
Published: 14 December 2009
... ]. For Fe/S cluster transfer holo-HCF101 was mixed with recombinant reduced apo-Leu1 [ 32 ]. Aliquots (5 µl) were analysed at various time points for Leu1 activity. [4Fe-4S]-cluster-containing P-loop NTPase (FSC-NTPase) Arabidopsis thaliana chloroplast high chlorophyll fluorescence 101 (HCF101...
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Biochem J (2009) 417 (1): 257–269.
Published: 12 December 2008
... of one of these subunits in Arabidopsis thaliana , ClpR1. Loss of ClpR1 caused a slow-growth phenotype, with chlorotic leaves during early development that later partially recovered upon maturity. Analysis of the Clp proteolytic core in the clpR1 mutant ( clpR1-1 ) revealed approx. 10% of the wild-type...
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Biochem J (2008) 415 (3): 387–399.
Published: 15 October 2008
... whether intact PtdIns(4,5) P 2 , Ins(1,4,5) P 3 or other derived metabolites represent physiological stress signals. Arabidopsis thaliana clathrin-coated vesicle (CCV) hyperosmotic stress lipid PtdIns(4,5) P 2 In the present paper, we show that PtdIns(4,5) P 2 formed as a result...
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Biochem J (2008) 415 (2): 247–255.
Published: 25 September 2008
... light exposure, whereas phyB–phyE are light-stable in both forms [ 6 ]. Arabidopsis thaliana dephosphorylation phosphatase phytochrome p hytochrome- a ssociated p rotein p hosphatase type 2C (PAPP2C) phytochrome-interacting factor 3 (PIF3) © The Authors Journal compilation ©...
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Biochem J (2008) 412 (2): 275–285.
Published: 14 May 2008
... an important role in the antioxidant system of plants. In the present study we addressed the significance of chloroplast APXs in stress tolerance and signalling in Arabidopsis thaliana . To this end, T-DNA (transfer DNA) insertion mutants tapx , sapx and tapx sapx , lacking the tAPX (thylakoid-bound APX), sAPX...
Includes: Supplementary data