Issues

An epifluorescence image of HEK293 cells expressing mKate2–GPER (red) and loaded with the Ca²⁺ indicator fura-2/AM (blue). The approach allows for direct comparison of Ca²⁺ signals in cells transfected with or without mKate2–GPER in the same microscopic field. In this issue of the Biochemical Journal, Terry et al. report on how activation of GPER (G protein-coupled estrogen receptor 1) “clamps” cytoplasmic Ca²⁺ signals via suppression of store-operated Ca²⁺ entry and Ca²⁺ efflux; see pages 3627–3642 for details.