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Volume 346, Issue 2

March 2000

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Cover Image

Cover Image
Front Matter
Table of Contents

ISSN 0264-6021

EISSN 1470-8728

In this Issue

Research communications
Research articles

Research communications

On the preparation of β-haematin

G. BLAUER; M. AKKAWI

Biochem J (2000) 346 (2): 249–250.

Abstract

View Articletitled, On the preparation of β-haematin

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The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin

Stewart D. NUTTALL; Simone E. DEUTSCHEL; Robert A. IRVING; Juan A. SERRANO-GOMICIA; Michael L. DYALL-SMITH

Biochem J (2000) 346 (2): 251–254.

Abstract

View Articletitled, The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin

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Research articles

Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli

Richard GRIEßLER; Sabato D'AURIA; Reinhard SCHINZEL; Fabio TANFANI; Bernd NIDETZKY

Biochem J (2000) 346 (2): 255–263.

Abstract

View Articletitled, Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli

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Some polyphenols inhibit the formation of pentyl radical and octanoic acid radical in the reaction mixture of linoleic acid hydroperoxide with ferrous ions

Hideo IWAHASHI

Biochem J (2000) 346 (2): 265–273.

Abstract

View Articletitled, Some polyphenols inhibit the formation of pentyl radical and octanoic acid radical in the reaction mixture of linoleic acid hydroperoxide with ferrous ions

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Ca2+ and calmodulin differentially modulate myo-inositol 1,4,5-trisphosphate (IP3)-binding to the recombinant ligand-binding domains of the various IP3 receptor isoforms

Sara VANLINGEN; Henk SIPMA; Patrick DE SMET; Geert CALLEWAERT; Ludwig MISSIAEN; Humbert DE SMEDT; Jan B. PARYS

Biochem J (2000) 346 (2): 275–280.

Abstract

View Articletitled, Ca2+ and calmodulin differentially modulate myo-inositol 1,4,5-trisphosphate (IP3)-binding to the recombinant ligand-binding domains of the various IP3 receptor isoforms

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Pancreatic eukaryotic initiation factor-2α kinase (PEK) homologues in humans, Drosophila melanogaster and Caenorhabditis elegans that mediate translational control in response to endoplasmic reticulum stress

Ruchira SOOD; Amy C. PORTER; Kun MA; Lawrence A. QUILLIAM; Ronald C. WEK

Biochem J (2000) 346 (2): 281–293.

Abstract

View Articletitled, Pancreatic eukaryotic initiation factor-2α kinase (PEK) homologues in humans, Drosophila melanogaster and Caenorhabditis elegans that mediate translational control in response to endoplasmic reticulum stress

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Target site search and effective inhibition of leukaemic cell growth by a covalently closed multiple anti-sense oligonucleotide to c-myb

Ik-Jae MOON; Youngik LEE; Chun-Sik KWAK; Je-Ho LEE; Kyusam CHOI; Alan D. SCHREIBER; Jong-Gu PARK

Biochem J (2000) 346 (2): 295–303.

Abstract

View Articletitled, Target site search and effective inhibition of leukaemic cell growth by a covalently closed multiple anti-sense oligonucleotide to c-myb

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HIV-2 protease is inactivated after oxidation at the dimer interface and activity can be partly restored with methionine sulphoxide reductase

David A. DAVIS; Fonda M. NEWCOMB; Jackob MOSKOVITZ; Paul T. WINGFIELD; Stephen J. STAHL; Joshua KAUFMAN; Henry M. FALES; Rodney L. LEVINE; Robert YARCHOAN

Biochem J (2000) 346 (2): 305–311.

Abstract

View Articletitled, HIV-2 protease is inactivated after oxidation at the dimer interface and activity can be partly restored with methionine sulphoxide reductase

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Inhibition of atrial natriuretic peptide (ANP) C receptor expression by antisense oligodeoxynucleotides in A10 vascular smooth-muscle cells is associated with attenuation of ANP-C-receptor-mediated inhibition of adenylyl cyclase

Anuradha PALAPARTI; Yuan LI; Madhu B. ANAND-SRIVASTAVA

Biochem J (2000) 346 (2): 313–320.

Abstract

View Articletitled, Inhibition of atrial natriuretic peptide (ANP) C receptor expression by antisense oligodeoxynucleotides in A10 vascular smooth-muscle cells is associated with attenuation of ANP-C-receptor-mediated inhibition of adenylyl cyclase

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Actin filaments play a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes

Waka OMATA; Hiroshi SHIBATA; Lu LI; Kuniaki TAKATA; Itaru KOJIMA

Biochem J (2000) 346 (2): 321–328.

Abstract

View Articletitled, Actin filaments play a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes

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Zinc-regulated ubiquitin conjugation signals endocytosis of the yeast ZRT1 zinc transporter

Raad S. GITAN; David J. EIDE

Biochem J (2000) 346 (2): 329–336.

Abstract

View Articletitled, Zinc-regulated ubiquitin conjugation signals endocytosis of the yeast ZRT1 zinc transporter

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Down-regulation of cyclic-nucleotide phosphodiesterase 3B in 3T3-L1 adipocytes induced by tumour necrosis factor α and cAMP

Tova RAHN LANDSTRÖM; Jie MEI; Marie KARLSSON; Vincent MANGANIELLO; Eva DEGERMAN

Biochem J (2000) 346 (2): 337–343.

Abstract

View Articletitled, Down-regulation of cyclic-nucleotide phosphodiesterase 3B in 3T3-L1 adipocytes induced by tumour necrosis factor α and cAMP

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Reagent or myeloperoxidase-generated hypochlorite affects discrete regions in lipid-free and lipid-associated human apolipoprotein A-I

Constanze BERGT; Karl OETTL; Walter KELLER; Fritz ANDREAE; Hans Jörg LEIS; Ernst MALLE; Wolfgang SATTLER

Biochem J (2000) 346 (2): 345–354.

Abstract

View Articletitled, Reagent or myeloperoxidase-generated hypochlorite affects discrete regions in lipid-free and lipid-associated human apolipoprotein A-I

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Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics

Rachel V. DUNN; Valerie RÉAT; John FINNEY; Michel FERRAND; Jeremy C. SMITH; Roy M. DANIEL

Biochem J (2000) 346 (2): 355–358.

Abstract

View Articletitled, Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics

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Stimulation of cleavage of membrane proteins by calmodulin inhibitors

Elena DÍAZ-RODRÍGUEZ; Azucena ESPARÍS-OGANDO; Juan Carlos MONTERO; Laura YUSTE; Atanasio PANDIELLA

Biochem J (2000) 346 (2): 359–367.

Abstract

View Articletitled, Stimulation of cleavage of membrane proteins by calmodulin inhibitors

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Stress-induced generation of N-acylethanolamines in mouse epidermal JB6 P+ cells

Evgueni V. BERDYSHEV; Patricia C. SCHMID; Zigang DONG; Harald H. O. SCHMID

Biochem J (2000) 346 (2): 369–374.

Abstract

View Articletitled, Stress-induced generation of N-acylethanolamines in mouse epidermal JB6 P+ cells

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Ferredoxin III of Desulfovibrio africanus: sequencing of the native gene and characterization of a histidine-tagged form

Johanneke L. H. BUSCH; Jacques L. BRETON; Sharon L. DAVY; Richard JAMES; Geoffrey R. MOORE; Fraser A. ARMSTRONG; Andrew J. THOMSON

Biochem J (2000) 346 (2): 375–384.

Abstract

View Articletitled, Ferredoxin III of Desulfovibrio africanus: sequencing of the native gene and characterization of a histidine-tagged form

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Estimation of systolic and diastolic free intracellular Ca2+ by titration of Ca2+ buffering in the ferret heart

Heide L. KIRSCHENLOHR; Andrew A. GRACE; Jamie I. VANDENBERG; James C. METCALFE; Gerry A. SMITH

Biochem J (2000) 346 (2): 385–391.

Abstract

View Articletitled, Estimation of systolic and diastolic free intracellular Ca2+ by titration of Ca2+ buffering in the ferret heart

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Ca2+ buffering in the heart: Ca2+ binding to and activation of cardiac myofibrils

Gerry A. SMITH; Henry B. F. DIXON; Heide L. KIRSCHENLOHR; Andrew A. GRACE; James C. METCALFE; Jamie I. VANDENBERG

Biochem J (2000) 346 (2): 393–402.

Abstract

View Articletitled, Ca2+ buffering in the heart: Ca2+ binding to and activation of cardiac myofibrils

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Thyroxine regulation of monolysocardiolipin acyltransferase activity in rat heart

Thomas MUTTER; Vernon W. DOLINSKY; Brian J. MA; William A. TAYLOR; Grant M. HATCH

Biochem J (2000) 346 (2): 403–406.

Abstract

View Articletitled, Thyroxine regulation of monolysocardiolipin acyltransferase activity in rat heart

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Continuous exposure to high concentrations of nitric oxide leads to persistent inhibition of oxygen consumption by J774 cells as well as extraction of oxygen by the extracellular medium

Antonia ORSI; Belén BELTRÁN; Emilio CLEMENTI; Katarina HALLÉN; Martin FEELISCH; Salvador MONCADA

Biochem J (2000) 346 (2): 407–412.

Abstract

View Articletitled, Continuous exposure to high concentrations of nitric oxide leads to persistent inhibition of oxygen consumption by J774 cells as well as extraction of oxygen by the extracellular medium

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Investigation of the mechanism by which glucose analogues cause translocation of glucokinase in hepatocytes: evidence for two glucose binding sites

Loranne AGIUS; Mark STUBBS

Biochem J (2000) 346 (2): 413–421.

Abstract

View Articletitled, Investigation of the mechanism by which glucose analogues cause translocation of glucokinase in hepatocytes: evidence for two glucose binding sites

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Identification and expression of an allergen Asp f 13 from Aspergillus fumigatus and epitope mapping using human IgE antibodies and rabbit polyclonal antibodies

Lu-Ping CHOW; Shu-Ling LIU; Chia-Jung YU; Hsin-Kai LIAO; Jaw-Ji TSAI; Tswen-Kei TANG

Biochem J (2000) 346 (2): 423–431.

Abstract

View Articletitled, Identification and expression of an allergen Asp f 13 from Aspergillus fumigatus and epitope mapping using human IgE antibodies and rabbit polyclonal antibodies

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Protein phosphatase 2A is associated in an inactive state with microtubules through 2A1-specific interaction with tubulin

Akira HIRAGA; Shinri TAMURA

Biochem J (2000) 346 (2): 433–439.

Abstract

View Articletitled, Protein phosphatase 2A is associated in an inactive state with microtubules through 2A1-specific interaction with tubulin

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Identification of a DNA-binding domain and an active-site residue of pseudorabies virus DNase

Tin-Yun HO; Shih-Lu WU; Chin-Hui HSIANG; Tien-Jye CHANG; Chien-Yun HSIANGǁ

Biochem J (2000) 346 (2): 441–445.

Abstract

View Articletitled, Identification of a DNA-binding domain and an active-site residue of pseudorabies virus DNase

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Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells

Robert C. GARDNER; Stephen J. ASSINDER; Gary CHRISTIE; Grant G. F. MASON; Roger MARKWELL; Harry WADSWORTH; Mark MCLAUGHLIN; Ron KING; Marie C. CHABOT-FLETCHER; John J. BRETON; David ALLSOP; A. Jennifer RIVETT

Biochem J (2000) 346 (2): 447–454.

Abstract

View Articletitled, Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells

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Involvement of Giα2 in sodium butyrate-induced erythroblastic differentiation of K562 cells

Mark G. DAVIS; Yumiko KAWAI; Ifeanyi J. ARINZE

Biochem J (2000) 346 (2): 455–461.

Abstract

View Articletitled, Involvement of Giα2 in sodium butyrate-induced erythroblastic differentiation of K562 cells

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Expression of heparan sulphate l-iduronyl 2-O-sulphotransferase in human kidney 293 cells results in increased d-glucuronyl 2-O-sulphation

Jianhui RONG; Hiroko HABUCHI; Koji KIMATA; Ulf LINDAHL; Marion KUSCHE-GULLBERG

Biochem J (2000) 346 (2): 463–468.

Abstract

View Articletitled, Expression of heparan sulphate l-iduronyl 2-O-sulphotransferase in human kidney 293 cells results in increased d-glucuronyl 2-O-sulphation

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Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methyl-esterified oligogalacturonates

Harry C. M. KESTER; Jacques A. E. BENEN; Jaap VISSER; Maria Esteban WARREN; Ron ORLANDO; Carl BERGMANN; Didier MAGAUD; Daniel ANKER; Alain DOUTHEAU

Biochem J (2000) 346 (2): 469–474.

Abstract

View Articletitled, Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methyl-esterified oligogalacturonates

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Cloning and functional characterization of the 5′-flanking region of human methionine adenosyltransferase 1A gene

Zhaohui ZENG; Zong-Zhi HUANG; Changjin CHEN; Heping YANG; Zebin MAO; Shelly C. LU

Biochem J (2000) 346 (2): 475–482.

Abstract

View Articletitled, Cloning and functional characterization of the 5′-flanking region of human methionine adenosyltransferase 1A gene

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The N-terminal 34 residues of the 55 kDa regulatory subunits of phosphoinositide 3-kinase interact with tubulin

Kouichi INUKAI; Makoto FUNAKI; Masao NAWANO; Hideki KATAGIRI; Takehide OGIHARA; Motonobu ANAI; Yukiko ONISHI; Hideyuki SAKODA; Hiraku ONO; Yasushi FUKUSHIMA; Masatoshi KIKUCHI; Yoshitomo OKA; Tomoichiro ASANO

Biochem J (2000) 346 (2): 483–489.

Abstract

View Articletitled, The N-terminal 34 residues of the 55 kDa regulatory subunits of phosphoinositide 3-kinase interact with tubulin

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Vitamin C protects against and reverses specific hypochlorous acid- and chloramine-dependent modifications of low-density lipoprotein

Anitra C. CARR; Terry TIJERINA; Balz FREI

Biochem J (2000) 346 (2): 491–499.

Abstract

View Articletitled, Vitamin C protects against and reverses specific hypochlorous acid- and chloramine-dependent modifications of low-density lipoprotein

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GTPase mechanism and function: new insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5

Zhimin LIANG; Timothy MATHER; Guangpu LI

Biochem J (2000) 346 (2): 501–508.

Abstract

View Articletitled, GTPase mechanism and function: new insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5

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Mutations in the reduced-folate carrier affect protein localization and stability

Heather SADLISH; Richard C. MURRAY; Frederick M. R. WILLIAMS; Wayne F. FLINTOFF

Biochem J (2000) 346 (2): 509–518.

Abstract

View Articletitled, Mutations in the reduced-folate carrier affect protein localization and stability

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Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts

Mark K. BOEHM; Adam L. CORPER; Tommy WAN; Maninder K. SOHI; Brian J. SUTTON; Jeremy D. THORNTON; Patricia A. KEEP; Kerry A. CHESTER; Richard H. J. BEGENT; Stephen J. PERKINS

Biochem J (2000) 346 (2): 519–528.

Abstract

View Articletitled, Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts

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Interaction between the homeodomain proteins Cdx2 and HNF1α mediates expression of the lactase-phlorizin hydrolase gene

Cathy MITCHELMORE; Jesper T. TROELSEN; Nikolaj SPODSBERG; Hans SJÖSTRÖM; Ove NORÉN

Biochem J (2000) 346 (2): 529–535.

Abstract

View Articletitled, Interaction between the homeodomain proteins Cdx2 and HNF1α mediates expression of the lactase-phlorizin hydrolase gene

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Rapid replenishment of sphingomyelin in the plasma membrane upon degradation by sphingomyelinase in NIH3T3 cells overexpressing the phosphatidylinositol transfer protein β

Claudia M. VAN TIEL; Chiara LUBERTO; Gerry T. SNOEK; Yusuf A. HANNUN; Karel W. A. WIRTZ

Biochem J (2000) 346 (2): 537–543.

Abstract

View Articletitled, Rapid replenishment of sphingomyelin in the plasma membrane upon degradation by sphingomyelinase in NIH3T3 cells overexpressing the phosphatidylinositol transfer protein β

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Plasmodium falciparum-infected red blood cells depend on a functional glutathione de novo synthesis attributable to an enhanced loss of glutathione

Kai LÜERSEN; Rolf D. WALTER; Sylke MÜLLER

Biochem J (2000) 346 (2): 545–552.

Abstract

View Articletitled, Plasmodium falciparum-infected red blood cells depend on a functional glutathione de novo synthesis attributable to an enhanced loss of glutathione

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Modulation of the glutathione S-transferase in Ochrobactrum anthropi: function of xenobiotic substrates and other forms of stress

Bartolo FAVALORO; Antonio TAMBURRO; Massimo A. TROFINO; Luciano BOLOGNA; Domenico ROTILIO; Hermann J. HEIPIEPER

Biochem J (2000) 346 (2): 553–559.

Abstract

View Articletitled, Modulation of the glutathione S-transferase in Ochrobactrum anthropi: function of xenobiotic substrates and other forms of stress

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