Plant organelles cope with endogenous DNA damaging agents, byproducts of respiration and photosynthesis, and exogenous agents like ultraviolet light. Plant organellar DNA polymerases (DNAPs) are not phylogenetically related to yeast and metazoan DNAPs and they harbor three insertions not present in any other DNAPs. Plant organellar DNAPs from Arabidopsis thaliana (AtPolIA and AtPolIB) are translesion synthesis (TLS) DNAPs able to bypass abasic sites, a lesion that poses a strong block to replicative polymerases. Besides abasic sites, reactive oxidative species and ionizing radiation react with thymine resulting in thymine glycol (Tg), a DNA adduct that is also a strong block to replication. Here, we report that AtPolIA and AtPolIB bypass Tg by inserting an adenine opposite the lesion and efficiently extend from a Tg-A base pair. The TLS ability of AtPolIB is mapped to two conserved lysine residues: K593 and K866. Residue K593 is situated in insertion 1 and K866 is in insertion 3. With basis on the location of both insertions on a structural model of AtPolIIB, we hypothesize that the two positively charged residues interact to form a clamp around the primer-template. In contrast with nuclear and bacterial replication, where lesion bypass involves an interplay between TLS and replicative DNA polymerases, we postulate that plant organellar DNAPs evolved to exert replicative and TLS activities.
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March 2020
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Native electrospray ionization mass spectrometry revealed coronaviral polyprotein processing by the viral protease and subsequent complex formation. For further information, see the article by Kirchel and colleagues (pp. 1009–1019) in this issue. The image was in parts created by Anne Rupprecht (Rostock, Germany) and compiled by Boris Krichel. Image provided by Charlotte Uetrecht.
Research Article|
March 13 2020
Plant organellar DNA polymerases bypass thymine glycol using two conserved lysine residues
Noe Baruch-Torres;
Noe Baruch-Torres
1Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP 36821, Irapuato, Guanajuato, Mexico
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Junpei Yamamoto;
Junpei Yamamoto
2Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
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Víctor Juárez-Quintero;
Víctor Juárez-Quintero
1Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP 36821, Irapuato, Guanajuato, Mexico
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Shigenori Iwai;
Shigenori Iwai
2Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
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Luis G. Brieba
1Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP 36821, Irapuato, Guanajuato, Mexico
Correspondence: (luis.brieba@cinvestav.mx)
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Publisher: Portland Press Ltd
Received:
January 15 2020
Revision Received:
February 25 2020
Accepted:
February 28 2020
Accepted Manuscript online:
February 28 2020
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem J (2020) 477 (5): 1049–1059.
Article history
Received:
January 15 2020
Revision Received:
February 25 2020
Accepted:
February 28 2020
Accepted Manuscript online:
February 28 2020
Citation
Noe Baruch-Torres, Junpei Yamamoto, Víctor Juárez-Quintero, Shigenori Iwai, Luis G. Brieba; Plant organellar DNA polymerases bypass thymine glycol using two conserved lysine residues. Biochem J 13 March 2020; 477 (5): 1049–1059. doi: https://doi.org/10.1042/BCJ20200043
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