Bardhan, I., Barman, S., Roy, A., and Sudhamalla, B. (2023) Novel insights into the recognition of acetylated histone H4 tail by the TRIM24 PHD-Bromo module. Biochem. J.480 (9): 629–647. doi: https://doi.org/10.1042/BCJ20230011

Due to an error in the production process, Table 1 was omitted from the published article. The complete Table 1 is presented here. Portland Press apologises for the error.

Table 1.
Binding affinities of the TRIM24 PHD-Bromo and Bromo alone to acetylated histone H4 peptides as measured by ITC
Histone peptidePeptide sequenceTRIM24-PHD-Bromo ITC KD (μM)TRIM24-Bromo ITC KD (μM)
H4(1-20)unmodified SGRGKGGKGLGKGGAKRHRK No binding No binding 
H4(1-15)K5ac SGRGKacGGKGLGKGGA 7.5 ± 1.0 6.5 ± 1.2 
H4(1-15)K8ac SGRGKGGKacGLGKGGA 4.2 ± 1.3 5.5 ± 1.0 
H4(1-15)K12ac SGRGKGGKGLGKacGGA  > 500  > 500 
H4(11-21)K16ac GKGGAKacRHRKVY 6.0 ± 1.0 6.5 ± 1.0 
H4(1-15)K5acK8ac SGRGKacGGKacGLGKGGA 4.2 ± 1.4 5.2 ± 1.0 
H4(1-15)K5acK12ac SGRGKacGGKGLGKacGGA 9.4 ± 1.0 8.6 ± 1.0 
H4(1-15)K8acK12ac SGRGKGGKacGLGKacGGA 8.6 ± 1.0 9.4 ± 1.2 
H4(1-20)Kac4 (K5/K8/K12/K16) SGRGKacGGKacGLGKacGGAKacRHRK 12.8 ± 1.0 14.5 ± 1.0 
Histone peptidePeptide sequenceTRIM24-PHD-Bromo ITC KD (μM)TRIM24-Bromo ITC KD (μM)
H4(1-20)unmodified SGRGKGGKGLGKGGAKRHRK No binding No binding 
H4(1-15)K5ac SGRGKacGGKGLGKGGA 7.5 ± 1.0 6.5 ± 1.2 
H4(1-15)K8ac SGRGKGGKacGLGKGGA 4.2 ± 1.3 5.5 ± 1.0 
H4(1-15)K12ac SGRGKGGKGLGKacGGA  > 500  > 500 
H4(11-21)K16ac GKGGAKacRHRKVY 6.0 ± 1.0 6.5 ± 1.0 
H4(1-15)K5acK8ac SGRGKacGGKacGLGKGGA 4.2 ± 1.4 5.2 ± 1.0 
H4(1-15)K5acK12ac SGRGKacGGKGLGKacGGA 9.4 ± 1.0 8.6 ± 1.0 
H4(1-15)K8acK12ac SGRGKGGKacGLGKacGGA 8.6 ± 1.0 9.4 ± 1.2 
H4(1-20)Kac4 (K5/K8/K12/K16) SGRGKacGGKacGLGKacGGAKacRHRK 12.8 ± 1.0 14.5 ± 1.0 
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© 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2023