Tomato powdery mildew, caused by Oidium neolycopersici, is a fungal disease that results in severe yield loss in infected plants. Herein, we describe the function of a class of proteins, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which play a role in vesicle transport during defense signaling. To date, there have been no reports describing the function of tomato SNAREs during resistance signaling to powdery mildew. Using a combination of classical plant pathology-, genetics-, and cell biology-based approaches, we evaluate the role of ShNPSN11 in resistance to the powdery mildew pathogen O. neolycopersici. Quantitative RT-PCR analysis of tomato SNAREs revealed that ShNPSN11 mRNA accumulation in disease-resistant varieties was significantly increased following pathogen, compared with susceptible varieties, suggesting a role during induced defense signaling. Using in planta subcellular localization, we demonstrate that ShNPSN11 was primarily localized at the plasma membrane, consistent with the localization of SNARE proteins and their role in defense signaling and trafficking. Silencing of ShNPSN11 resulted in increased susceptibility to O. neolycopersici, with pathogen-induced levels of H2O2 and cell death elicitation in ShNPSN11-silenced lines showing a marked reduction. Transient expression of ShNPSN11 did not result in the induction of a hypersensitive cell death response or suppress cell death induced by BAX. Taken together, these data demonstrate that ShNPSNl11 plays an important role in defense activation and host resistance to O. neolycopersici in tomato LA1777.
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October 2020
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Cover Image
The cover image showing the crystal structure of human hemoglobin (PDB: 1GZX). For more information, see the article by Natarajan et al. (pp. 3839–3850). Artwork Image courtesy of C. Natarajan.
Research Article|
October 12 2020
ShNPSN11, a vesicle-transport-related gene, confers disease resistance in tomato to Oidium neolycopersici
Qinggui Lian
;
Qinggui Lian
*
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
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Yanan Meng;
Yanan Meng
*
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
2College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China
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Xinbei Zhao;
Xinbei Zhao
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
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Yuanliu Xu;
Yuanliu Xu
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
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Yang Wang;
Yang Wang
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
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Brad Day;
3Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, U.S.A.
4Plant Resilience Institute, Michigan State University, East Lansing, MI 48824, U.S.A.
Correspondence: Brad Day (bday@msu.edu) or Qing Ma (maqing@nwsuaf.edu.cn)
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Qing Ma
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
Correspondence: Brad Day (bday@msu.edu) or Qing Ma (maqing@nwsuaf.edu.cn)
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Publisher: Portland Press Ltd
Received:
October 22 2019
Revision Received:
September 11 2020
Accepted:
September 21 2020
Accepted Manuscript online:
September 21 2020
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem J (2020) 477 (19): 3851–3866.
Article history
Received:
October 22 2019
Revision Received:
September 11 2020
Accepted:
September 21 2020
Accepted Manuscript online:
September 21 2020
Citation
Qinggui Lian, Yanan Meng, Xinbei Zhao, Yuanliu Xu, Yang Wang, Brad Day, Qing Ma; ShNPSN11, a vesicle-transport-related gene, confers disease resistance in tomato to Oidium neolycopersici. Biochem J 16 October 2020; 477 (19): 3851–3866. doi: https://doi.org/10.1042/BCJ20190776
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