The pluripotency factor, OCT4 gene is a stemness marker that is involved in the tumorigenicity of different cancer types and knowing about molecular mechanisms of its regulation is crucially important. To date, a few microRNAs (miRNAs) are known to be regulators of OCT4 gene expression. Looking for the novel miRNAs which are capable of regulating OCT4 gene expression, our bioinformatics analysis introduced hsa-miR-3658 (miR-3658) as a bona fide candidate. Then, RT-qPCR results indicated that miR-3658 expression is decreased in colorectal cancer (CRC) tumor tissues, compared with normal pairs. Furthermore, RT-qPCR and western blot analysis showed that the OCT4 gene has been down-regulated following the miR-3658 overexpression. Consistently, dual-luciferase assay supported the direct interaction of miR-3658 with the 3′-UTR sequence of OCT4 gene. Unlike in HCT116 cells, overexpression of miR-3658 in SW480 cells brought about growth inhibition, cell cycle arrest and reduced cell migration, detected by flow cytometry, and scratch test assay. Overall, these findings demonstrated that miR-3658 as a tumor suppressor miRNA exerts its effect against OCT4 gene expression, and it has the potential of being used as a prognostic marker and therapeutic target against colorectal cancer.
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Cover Image
The Autographa californica Multiple Nucleo-Polyhedrosis virus encodes for a variant of Ubiquitin molecule that can create atypical linkages mediated by Lysine 54 (shown in red). We show that the Ubiquitin signalling via the atypical chains is protected from the host Deubiquitinase enzymes, which possibly allows the virus to circumvent antiviral responses. For more information, see the article by Negi and colleagues in this issue (pp. 2193–2219). The image was provided by Ranabir Das.
Hsa-miR-3658 down-regulates OCT4 gene expression followed by suppressing SW480 cell proliferation and migration
Fahimeh Hosseini, Bahram M. Soltani, Hossein Baharvand, Saman Hosseinkhani; Hsa-miR-3658 down-regulates OCT4 gene expression followed by suppressing SW480 cell proliferation and migration. Biochem J 26 June 2020; 477 (12): 2281–2293. doi: https://doi.org/10.1042/BCJ20190619
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