Phosphatase of regenerating liver (PRL) is highly expressed in malignant cancers and promotes cancer progression. Recent studies have suggested its functional relationship with Mg2+, but the importance and molecular details of this relationship remain unknown. Here, we report that PRL expression is regulated by Mg2+ and PRL protects cells from apoptosis under Mg2+-depleted conditions. When cultured cells were subjected to Mg2+ depletion, endogenous PRL protein levels increased significantly. siRNA-mediated knockdown of endogenous PRL did not significantly affect cell proliferation under normal culture conditions, but it increased cell death after Mg2+ depletion. Imaging analyses with a fluorescent probe for Mg2+ showed that PRL knockdown severely reduced intracellular Mg2+ levels, indicating a role for PRL in maintaining intracellular Mg2+. We also examined the mechanism of augmented expression of PRL proteins and found that PRL mRNA transcription was stimulated by Mg2+ depletion. A series of analyses revealed the activation and the crucial importance of signal transducer and activator of transcription 1 in this process. Collectively, these results implicate PRL in maintaining cellular Mg2+ homeostasis.
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March 2018
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Endoplasmic reticulum (ER) surrounding a cell nucleus. In this issue of the Biochemical Journal, Stupka et al. discuss the role of ER selenoproteins in the regulation of cellular stress responses. For further information, see pages 1037–1057.
Research Article|
March 26 2018
Phosphatase of regenerating liver maintains cellular magnesium homeostasis
Atsushi Yoshida;
Atsushi Yoshida
1Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
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Yosuke Funato;
Yosuke Funato
1Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
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Hiroaki Miki
1Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
Correspondence: Hiroaki Miki (hmiki@biken.osaka-u.ac.jp)
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Publisher: Portland Press Ltd
Received:
October 05 2017
Revision Received:
February 16 2018
Accepted:
February 27 2018
Accepted Manuscript online:
February 27 2018
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Biochem J (2018) 475 (6): 1129–1139.
Article history
Received:
October 05 2017
Revision Received:
February 16 2018
Accepted:
February 27 2018
Accepted Manuscript online:
February 27 2018
Citation
Atsushi Yoshida, Yosuke Funato, Hiroaki Miki; Phosphatase of regenerating liver maintains cellular magnesium homeostasis. Biochem J 30 March 2018; 475 (6): 1129–1139. doi: https://doi.org/10.1042/BCJ20170756
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