The cockroach, which is a household insect, is an established model organism in research. Periplanetasin-2, derived from the American cockroach Periplaneta americana, exerted potent antifungal effect against pathogenic fungi without causing hemolysis. Periplanetasin-2 induced oxidative stress by generation of reactive oxygen species (ROS) and lipid peroxidation. Periplanetasin-2 also caused apoptosis by exposure of phosphatidylserine and fragmentation of DNA, exerted in a concentration-dependent manner. Hence, we investigated the mitochondrial apoptotic mechanism of periplanetasin-2 in Candida albicans. After treatment with periplanetasin-2, we observed mitochondrial depolarization and calcium accumulation. Moreover, we observed a decrease in cytosolic glutathione, and an increase in mitochondrial glutathione, indicating that periplanetasin-2 induced oxidative stress and high ROS production in the mitochondria. Because of this mitochondrial dysfunction, cytochrome c was released from the mitochondria into the cytosol, and caspase was activated in a time-dependent manner. In summary, the antifungal peptide periplanetasin-2 activates apoptotic signals in the mitochondria by induction of oxidative stress.
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September 2017
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Cover Image
The American cockroach (Periplaneta americana). In this issue of the Biochemical Journal, Yun et al. describe the antifungal activity of the peptide, periplanetasin-2 from P. americana (for details, see pages 3027–3043)
Research Article|
August 22 2017
The antifungal activity of the peptide, periplanetasin-2, derived from American cockroach Periplaneta americana
JiEun Yun;
JiEun Yun
*
1School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea
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Jae-Sam Hwang;
Jae-Sam Hwang
*
2Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 55365, Republic of Korea
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Dong Gun Lee
1School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea
Correspondence: Dong Gun Lee (dglee222@knu.ac.kr)
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Publisher: Portland Press Ltd
Received:
June 07 2017
Revision Received:
July 19 2017
Accepted:
July 20 2017
Accepted Manuscript online:
July 21 2017
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2017
Biochem J (2017) 474 (17): 3027–3043.
Article history
Received:
June 07 2017
Revision Received:
July 19 2017
Accepted:
July 20 2017
Accepted Manuscript online:
July 21 2017
Citation
JiEun Yun, Jae-Sam Hwang, Dong Gun Lee; The antifungal activity of the peptide, periplanetasin-2, derived from American cockroach Periplaneta americana. Biochem J 1 September 2017; 474 (17): 3027–3043. doi: https://doi.org/10.1042/BCJ20170461
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