Many secreted bacterial effector proteins play a critical role in host–pathogen interactions by mediating a variety of post-translational modifications, some of which do not occur natively within the eukaryotic proteome. The characterization of bacterial effector protein activity remains an important step to understanding the subversion of host cell biology during pathogen infection and although molecular biology and immunochemistry remain critical tools for gaining insights into bacterial effector functions, increasingly mass spectrometry (MS) and proteomic approaches are also playing an indispensable role. The focus of this editorial is to highlight the strengths of specific MS approaches and their utility for the characterization of bacterial effector activity. With the capability of new generation MS instrumentation, MS-based technologies can provide information that is inaccessible using traditional molecular or immunochemical approaches.
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August 2017
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The distribution of haemagglutinin (green) and β-catenin (red) in MDCK cells overexpressing Akt1-HA. In this issue of the Biochemical Journal, Castañeda et al. report on the suppression of Akt/β-catenin-mediated cell proliferation by the the inhibition of 14-3-3ζ expression (see pages 2679–2689).
Editorial|
August 07 2017
The role of mass spectrometry analysis in bacterial effector characterization
Nichollas E. Scott;
Nichollas E. Scott
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne 3000, Australia
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Elizabeth L. Hartland
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne 3000, Australia
Correspondence: Elizabeth L. Hartland (hartland@unimelb.edu.au)
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Publisher: Portland Press Ltd
Received:
February 18 2017
Revision Received:
June 26 2017
Accepted:
July 06 2017
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2017
Biochem J (2017) 474 (16): 2779–2784.
Article history
Received:
February 18 2017
Revision Received:
June 26 2017
Accepted:
July 06 2017
Citation
Nichollas E. Scott, Elizabeth L. Hartland; The role of mass spectrometry analysis in bacterial effector characterization. Biochem J 15 August 2017; 474 (16): 2779–2784. doi: https://doi.org/10.1042/BCJ20160797
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