Human fibrinogen is an important coagulation factor as well as an independent predictor of coronary heart disease and stroke. Analysis of dysfibrinogens may provide useful information and help us to understand the molecular defects in fibrin polymerization. In the present study, we investigated the influence of oxidative stress of fibrinogen induced by H2O2 on the polymerization state of fibrin. UV absorbance spectroscopy, circular dichroism, ζ-potential, dynamic light scattering and steady shear viscosity were all employed to study the influence of oxidative stress on the molecular structure, the surface charges, and the size and shape of fibrinogen molecules. The fibrin morphology obtained was imaged and investigated using atomic force microscopy. The results demonstrated that the cross-linking, branching and height distribution of formed fibrin will be influenced by the oxidative stress of fibrinogen. This study presents new insights into the aggregation behaviour of fibrinogen and will be helpful to understand the formation mechanism of thrombosis under oxidative stress.
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December 2016
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Cover Image
Structure of the C-terminal part of C. elegans cyclase-associated protein (CAS-2) (top) was modeled as a dimer based on the structure of the C-terminal part of human CAP1 (bottom). Mutagenesis study revealed an essential role of the C-terminal dimerization motif for the actin-regulatory activities of CAS-2. Please see pp. 4427–4441 for further information. Image provided by S. Ono.
Research Article|
November 25 2016
Study on the influence of oxidative stress on the fibrillization of fibrinogen
Lei Wang;
Lei Wang
1Department of Chemistry, Liaocheng University, Liaocheng 252000, China
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Lianzhi Li;
Lianzhi Li
1Department of Chemistry, Liaocheng University, Liaocheng 252000, China
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Huaisheng Wang;
Huaisheng Wang
1Department of Chemistry, Liaocheng University, Liaocheng 252000, China
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Jifeng Liu
Jifeng Liu
2Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China
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Publisher: Portland Press Ltd
Received:
July 21 2016
Revision Received:
September 27 2016
Accepted:
October 04 2016
Accepted Manuscript online:
October 04 2016
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society
2016
Biochem J (2016) 473 (23): 4373–4384.
Article history
Received:
July 21 2016
Revision Received:
September 27 2016
Accepted:
October 04 2016
Accepted Manuscript online:
October 04 2016
Citation
Lei Wang, Lianzhi Li, Huaisheng Wang, Jifeng Liu; Study on the influence of oxidative stress on the fibrillization of fibrinogen. Biochem J 1 December 2016; 473 (23): 4373–4384. doi: https://doi.org/10.1042/BCJ20160702
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