Obesity affects the functional capability of adipose-derived stem cells (ASCs) and their effective use in regenerative medicine through mechanisms that are still poorly understood. In the present study we used a multiplatform [LC/MS, GC/MS and capillary electrophoresis/MS (CE/MS)], metabolomics, untargeted approach to investigate the metabolic alteration underlying the inequalities observed in obesity-derived ASCs. The metabolic fingerprint (metabolites within the cells) and footprint (metabolites secreted in the culture medium), from obesity- and non-obesity-derived ASCs of humans or mice, were characterized to provide valuable information. Metabolites associated with glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and the polyol pathway were increased in the footprint of obesity-derived human ASCs, indicating alterations in carbohydrate metabolism, whereas, from the murine model, deep differences in lipid and amino acid catabolism were highlighted. Therefore, new insights on the ASCs’ metabolome were provided that enhance our understanding of the processes underlying ASCs’ stemness capacity and its relationship with obesity, in different cell models.
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Research Article|
July 12 2016
New insight on obesity and adipose-derived stem cells using comprehensive metabolomics
Annalaura Mastrangelo;
Annalaura Mastrangelo
*Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain
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María I. Panadero;
María I. Panadero
*Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain
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Laura M. Pérez;
Laura M. Pérez
†Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain
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Beatriz G. Gálvez;
Beatriz G. Gálvez
†Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain
‡School of Doctoral Studies & Research, Universidad Europea (UE), Madrid, Spain
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Antonia García;
Antonia García
*Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain
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Coral Barbas;
Coral Barbas
*Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain
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Francisco J. Rupérez
Francisco J. Rupérez
1
*Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain
1To whom correspondence should be addressed (email ruperez@ceu.es).
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Publisher: Portland Press Ltd
Received:
March 19 2016
Revision Received:
April 28 2016
Accepted:
May 19 2016
Accepted Manuscript online:
May 19 2016
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
Biochem J (2016) 473 (14): 2187–2203.
Article history
Received:
March 19 2016
Revision Received:
April 28 2016
Accepted:
May 19 2016
Accepted Manuscript online:
May 19 2016
Citation
Annalaura Mastrangelo, María I. Panadero, Laura M. Pérez, Beatriz G. Gálvez, Antonia García, Coral Barbas, Francisco J. Rupérez; New insight on obesity and adipose-derived stem cells using comprehensive metabolomics. Biochem J 15 July 2016; 473 (14): 2187–2203. doi: https://doi.org/10.1042/BCJ20160241
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