Selenophosphate synthetase (SPS) was initially detected in bacteria and was shown to synthesize selenophosphate, the active selenium donor. However, mammals have two SPS paralogues, which are designated SPS1 and SPS2. Although it is known that SPS2 catalyses the synthesis of selenophosphate, the function of SPS1 remains largely unclear. To examine the role of SPS1 in mammals, we generated a Sps1-knockout mouse and found that systemic SPS1 deficiency led to embryos that were clearly underdeveloped by embryonic day (E)8.5 and virtually resorbed by E14.5. The knockout of Sps1 in the liver preserved viability, but significantly affected the expression of a large number of mRNAs involved in cancer, embryonic development and the glutathione system. Particularly notable was the extreme deficiency of glutaredoxin 1 (GLRX1) and glutathione transferase Omega 1 (GSTO1). To assess these phenotypes at the cellular level, we targeted the removal of SPS1 in F9 cells, a mouse embryonal carcinoma (EC) cell line, which affected the glutathione system proteins and accordingly led to the accumulation of hydrogen peroxide in the cell. Furthermore, we found that several malignant characteristics of SPS1-deficient F9 cells were reversed, suggesting that SPS1 played a role in supporting and/or sustaining cancer. In addition, the overexpression of mouse or human GLRX1 led to a reversal of observed increases in reactive oxygen species (ROS) in the F9 SPS1/GLRX1-deficient cells and resulted in levels that were similar to those in F9 SPS1-sufficient cells. The results suggested that SPS1 is an essential mammalian enzyme with roles in regulating redox homoeostasis and controlling cell growth.
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Research Article|
July 12 2016
Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals
Ryuta Tobe;
Ryuta Tobe
1
*Molecular Biology of Selenium, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Bradley A. Carlson;
Bradley A. Carlson
1
*Molecular Biology of Selenium, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Jang Hoe Huh;
Jang Hoe Huh
1
†School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
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Nadia P. Castro;
Nadia P. Castro
‡Tumor Growth Factor Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Xue-Ming Xu;
Xue-Ming Xu
2
*Molecular Biology of Selenium, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Petra A. Tsuji;
Petra A. Tsuji
§Department of Biological Sciences, Towson University, Towson, MD 21252, U.S.A.
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Sang-Goo Lee;
Sang-Goo Lee
║Division of Genetics, Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, U.S.A.
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Jeyoung Bang;
Jeyoung Bang
†School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
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Ji-Woon Na;
Ji-Woon Na
†School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
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Young-Yun Kong;
Young-Yun Kong
†School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
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Daniel Beaglehole;
Daniel Beaglehole
*Molecular Biology of Selenium, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Eileen Southon;
Eileen Southon
¶Basic Science Program, SAIC-Frederick, NCI-Frederick, Frederick, MD 21702, U.S.A.
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Harold Seifried;
Harold Seifried
**Nutritional Science Research Group, National Cancer Institute, Rockville, MD 20892, U.S.A.
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Lino Tessarollo;
Lino Tessarollo
††Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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David S. Salomon;
David S. Salomon
‡Tumor Growth Factor Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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Ulrich Schweizer;
Ulrich Schweizer
‡‡Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, Germany
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Vadim N. Gladyshev;
Vadim N. Gladyshev
║Division of Genetics, Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, U.S.A.
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Dolph L. Hatfield;
Dolph L. Hatfield
3
*Molecular Biology of Selenium, Mouse Cancer Genetics Program, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, U.S.A.
3Correspondence may be addressed to either of these authors (email hatfield@mail.nih.gov or imbglmg@snu.ac.kr).
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Byeong Jae Lee
Byeong Jae Lee
3
†School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
3Correspondence may be addressed to either of these authors (email hatfield@mail.nih.gov or imbglmg@snu.ac.kr).
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Publisher: Portland Press Ltd
Received:
November 20 2015
Revision Received:
May 13 2016
Accepted:
May 16 2016
Accepted Manuscript online:
May 16 2016
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
Biochem J (2016) 473 (14): 2141–2154.
Article history
Received:
November 20 2015
Revision Received:
May 13 2016
Accepted:
May 16 2016
Accepted Manuscript online:
May 16 2016
Citation
Ryuta Tobe, Bradley A. Carlson, Jang Hoe Huh, Nadia P. Castro, Xue-Ming Xu, Petra A. Tsuji, Sang-Goo Lee, Jeyoung Bang, Ji-Woon Na, Young-Yun Kong, Daniel Beaglehole, Eileen Southon, Harold Seifried, Lino Tessarollo, David S. Salomon, Ulrich Schweizer, Vadim N. Gladyshev, Dolph L. Hatfield, Byeong Jae Lee; Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals. Biochem J 15 July 2016; 473 (14): 2141–2154. doi: https://doi.org/10.1042/BCJ20160393
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