HOX (homeobox) genes encode a family of transcriptional regulators, which have an important role in morphogenesis and differentiation during embryonic development. Their deregulated expression is involved in the carcinogenesis of many human solid tumours. In the present study, we show that HOXB5 mRNA was significantly overexpressed in gastric cancer tissues compared with adjacent normal tissues. HOXB5-up-regulated cancer cells showed increased invasion and migration activity, but no change in proliferation activity, whereas HOXB5-down-regulated cells showed decreased invasion and migration activity. Up-regulation of HOXB5 resulted in up-regulation of β-catenin, whereas inhibition of HOXB5 expression by siRNA led to the down-regulation of β-catenin. Moreover, a significant correlation between HOXB5 and CTNNB1 (β-catenin) mRNA expression was detected in gastric cancer tissues. Furthermore, we found that HOXB5 binds directly to the CTNNB1 promoter region and activates the transcriptional expression of β-catenin, as well as its downstream target genes, encoding cyclin D1 and c-Myc, leading to an increase in the invasion and migration activity of human gastric cancer cells. Thus HOXB5 may be an important regulator of the Wnt/β-catenin signalling pathway, thereby contributing to gastric cancer progression and metastasis.
Skip Nav Destination
Article navigation
December 2015
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
November 27 2015
HOXB5 induces invasion and migration through direct transcriptional up-regulation of β-catenin in human gastric carcinoma
Chang-Soo Hong;
Chang-Soo Hong
1
*Research Center for Molecular Therapeutic to GI Tract, Institute for Biomedical Science, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
Search for other works by this author on:
Oh Jeong;
Oh Jeong
1
†Department of Surgery, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
Search for other works by this author on:
Zhengri Piao;
Zhengri Piao
2
*Research Center for Molecular Therapeutic to GI Tract, Institute for Biomedical Science, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
‡Center for Creative Biomedical Scientists (BK-21 Plus Project), Chonnam National University Medical School, Gwangju 501-746, Republic of Korea
Search for other works by this author on:
Chen Guo;
Chen Guo
3
*Research Center for Molecular Therapeutic to GI Tract, Institute for Biomedical Science, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
Search for other works by this author on:
Mi-Ran Jung;
Mi-Ran Jung
†Department of Surgery, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
Search for other works by this author on:
Chan Choi;
Chan Choi
§Department of Pathology, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
Search for other works by this author on:
Young-Kyu Park
Young-Kyu Park
4
*Research Center for Molecular Therapeutic to GI Tract, Institute for Biomedical Science, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
†Department of Surgery, Chonnam National University Hwasun Hospital, Jeonnam 519-763, Republic of Korea
‡Center for Creative Biomedical Scientists (BK-21 Plus Project), Chonnam National University Medical School, Gwangju 501-746, Republic of Korea
4To whom correspondence should be addressed (email parkyk@jnu.ac.kr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
February 16 2015
Revision Received:
October 01 2015
Accepted:
October 14 2015
Accepted Manuscript online:
October 14 2015
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2015 Authors; published by Portland Press Limited
2015
Biochem J (2015) 472 (3): 393–403.
Article history
Received:
February 16 2015
Revision Received:
October 01 2015
Accepted:
October 14 2015
Accepted Manuscript online:
October 14 2015
Citation
Chang-Soo Hong, Oh Jeong, Zhengri Piao, Chen Guo, Mi-Ran Jung, Chan Choi, Young-Kyu Park; HOXB5 induces invasion and migration through direct transcriptional up-regulation of β-catenin in human gastric carcinoma. Biochem J 15 December 2015; 472 (3): 393–403. doi: https://doi.org/10.1042/BJ20150213
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.