miRNAs regulate protein abundance and control diverse aspects of cellular processes and biological functions in metabolic diseases, such as obesity and type 2 diabetes (T2D). Let (lethal)-7 miRNAs specifically targets genes associated with T2D and have been implicated in the regulation of peripheral glucose metabolism, yet the direct regulators of let-7 miRNA expression are unknown. In the present study, we report on a putative promoter region for the let-7a-1, let-7f-1 and let-7d gene cluster on chromosome 9 and characterize the promoter activity of this novel area. We show that promoter activity and let-7 miRNA expression is dynamically regulated in response to different factors including serum, glucose, tumour necrosis factor (TNF)-α and caffeine. These findings will contribute to understanding the interaction between precise promoter elements to control the transcription and translation of let-7 miRNA genes.
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Research Article|
November 13 2015
miRNA let-7 expression is regulated by glucose and TNF-α by a remote upstream promoter
Mutsumi Katayama;
Mutsumi Katayama
*Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 77 Stockholm, Sweden
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Rasmus J.O. Sjögren;
Rasmus J.O. Sjögren
†Department of Molecular Medicine and Surgery, Karolinska Institutet, SE-171 77 Stockholm, Sweden
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Brendan Egan;
Brendan Egan
†Department of Molecular Medicine and Surgery, Karolinska Institutet, SE-171 77 Stockholm, Sweden
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Anna Krook
Anna Krook
1
*Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 77 Stockholm, Sweden
1To whom correspondence should be addressed (email Anna.Krook@ki.se).
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Publisher: Portland Press Ltd
Received:
February 20 2015
Revision Received:
September 01 2015
Accepted:
September 16 2015
Accepted Manuscript online:
September 16 2015
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2015 Authors; published by Portland Press Limited
2015
Biochem J (2015) 472 (2): 147–156.
Article history
Received:
February 20 2015
Revision Received:
September 01 2015
Accepted:
September 16 2015
Accepted Manuscript online:
September 16 2015
Citation
Mutsumi Katayama, Rasmus J.O. Sjögren, Brendan Egan, Anna Krook; miRNA let-7 expression is regulated by glucose and TNF-α by a remote upstream promoter. Biochem J 1 December 2015; 472 (2): 147–156. doi: https://doi.org/10.1042/BJ20150224
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