Phosphofructokinase-1 (Pfk) acts as the main control point of flux through glycolysis. It is involved in complex allosteric regulation and Pfk mutations have been linked to cancer development. Whereas the 3D structure and structural basis of allosteric regulation of prokaryotic Pfk has been studied in great detail, our knowledge about the molecular basis of the allosteric behaviour of the more complex mammalian Pfk is still very limited. To characterize the structural basis of allosteric regulation, the subunit interfaces and the functional consequences of modifications in Tarui's disease and cancer, we analysed the physiological homotetramer of human platelet Pfk at up to 2.67 Å resolution in two crystal forms. The crystallized enzyme is permanently activated by a deletion of the 22 C-terminal residues. Complex structures with ADP and fructose-6-phosphate (F6P) and with ATP suggest a role of three aspartates in the deprotonation of the OH-nucleophile of F6P and in the co-ordination of the catalytic magnesium ion. Changes at the dimer interface, including an asymmetry observed in both crystal forms, are the primary mechanism of allosteric regulation of Pfk by influencing the F6P-binding site. Whereas the nature of this conformational switch appears to be largely conserved in bacterial, yeast and mammalian Pfk, initiation of these changes differs significantly in eukaryotic Pfk.
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Research Article|
July 23 2015
Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation
Marco Kloos;
Marco Kloos
*Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany
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Antje Brüser;
Antje Brüser
†Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
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Jürgen Kirchberger;
Jürgen Kirchberger
†Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
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Torsten Schöneberg;
Torsten Schöneberg
†Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
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Norbert Sträter
Norbert Sträter
1
*Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany
1To whom correspondence should be addressed (email strater@bbz.uni-leipzig.de).
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Publisher: Portland Press Ltd
Received:
February 25 2015
Revision Received:
May 20 2015
Accepted:
June 01 2015
Accepted Manuscript online:
June 10 2015
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2015 Authors; published by Portland Press Limited
2015
Biochem J (2015) 469 (3): 421–432.
Article history
Received:
February 25 2015
Revision Received:
May 20 2015
Accepted:
June 01 2015
Accepted Manuscript online:
June 10 2015
Citation
Marco Kloos, Antje Brüser, Jürgen Kirchberger, Torsten Schöneberg, Norbert Sträter; Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation. Biochem J 1 August 2015; 469 (3): 421–432. doi: https://doi.org/10.1042/BJ20150251
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