Signalling pathways mediated by heterotrimeric G-proteins are common to all eukaryotes. Plants have a limited number of each of the G-protein subunits, with the most elaborate G-protein network discovered so far in soya bean (Glycine max, also known as soybean) which has four Gα, four Gβ and ten Gγ proteins. Biochemical characterization of Gα proteins from plants suggests significant variation in their properties compared with the well-characterized non-plant proteins. Furthermore, the four soya bean Gα (GmGα) proteins exhibit distinct biochemical activities among themselves, but the extent to which such biochemical differences contribute to their in vivo function is also not known. We used the yeast gpa1 mutant which displays constitutive signalling and growth arrest in the pheromone-response pathway as an in vivo model to evaluate the effect of distinct biochemical activities of GmGα proteins. We showed that specific GmGα proteins can be activated during pheromone-dependent receptor-mediated signalling in yeast and they display different strengths towards complementation of yeast gpa1 phenotypes. We also identified amino acids that are responsible for differential complementation abilities of specific Gα proteins. These data establish that specific plant Gα proteins are functional in the receptor-mediated pheromone-response pathway in yeast and that the subtle biochemical differences in their activity are physiologically relevant.
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Research Article|
June 13 2014
Soya bean Gα proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant
Swarup Roy Choudhury;
Swarup Roy Choudhury
*Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, U.S.A.
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Yuqi Wang;
Yuqi Wang
†Department of Biology, Saint Louis University, 3507 Laclede Avenue, St. Louis, MO 63103, U.S.A.
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Sona Pandey
Sona Pandey
1
*Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, U.S.A.
1To whom correspondence should be addressed (email spandey@danforthcenter.org).
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Publisher: Portland Press Ltd
Received:
October 08 2013
Revision Received:
March 27 2014
Accepted:
April 02 2014
Accepted Manuscript online:
April 02 2014
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem J (2014) 461 (1): 75–85.
Article history
Received:
October 08 2013
Revision Received:
March 27 2014
Accepted:
April 02 2014
Accepted Manuscript online:
April 02 2014
Citation
Swarup Roy Choudhury, Yuqi Wang, Sona Pandey; Soya bean Gα proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant. Biochem J 1 July 2014; 461 (1): 75–85. doi: https://doi.org/10.1042/BJ20131341
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