Trehalase, which specifically hydrolyses trehalose into glucose, plays an important role in the metabolism of trehalose. Large amounts of trehalose are stored in the diapause encysted embryos (cysts) of Artemia, which are not only vital to their extraordinary stress resistance, but also provide a source of energy for development after diapause is terminated. In the present study, a mechanism for the transcriptional regulation of trehalase was described in Artemia parthenogenetica. A trehalase-associated protein (ArTAP) was identified in Artemia-producing diapause cysts. ArTAP was found to be expressed only in diapause-destined embryos. Further analyses revealed that ArTAP can bind to a specific intronic segment of a trehalase gene. Knockdown of ArTAP by RNAi resulted in the release of cysts with coarse shells in which two chitin-binding proteins were missing. Western blotting showed that the level of trehalase was increased and apoptosis was induced in these ArTAP-knockdown cysts compared with controls. Taken together, these results show that ArTAP is a key regulator of trehalase expression which, in turn, plays an important role in trehalose metabolism during the formation of diapause cysts.
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Research Article|
November 08 2013
Regulation of trehalase expression inhibits apoptosis in diapause cysts of Artemia
Fan Yang;
Fan Yang
1
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
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Su Chen;
Su Chen
1
†Hangzhou Wahaha Technology Co. Ltd. Street 14, Xiasha Economic and Technological Development Zone, Hangzhou 310018, Zhejiang, People's Republic of China
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Zhong-Min Dai;
Zhong-Min Dai
‡Institute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, People's Republic of China
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Dian-Fu Chen;
Dian-Fu Chen
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
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Ru-Bing Duan;
Ru-Bing Duan
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
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Hong-Liang Wang;
Hong-Liang Wang
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
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Sheng-Nan Jia;
Sheng-Nan Jia
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
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Wei-Jun Yang
Wei-Jun Yang
2
*Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, Zhejiang, People's Republic of China
2To whom correspondence should be addressed (email w_jyang@cls.zju.edu.cn).
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Publisher: Portland Press Ltd
Received:
July 31 2013
Revision Received:
September 24 2013
Accepted:
September 25 2013
Accepted Manuscript online:
September 25 2013
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem J (2013) 456 (2): 185–194.
Article history
Received:
July 31 2013
Revision Received:
September 24 2013
Accepted:
September 25 2013
Accepted Manuscript online:
September 25 2013
Citation
Fan Yang, Su Chen, Zhong-Min Dai, Dian-Fu Chen, Ru-Bing Duan, Hong-Liang Wang, Sheng-Nan Jia, Wei-Jun Yang; Regulation of trehalase expression inhibits apoptosis in diapause cysts of Artemia. Biochem J 1 December 2013; 456 (2): 185–194. doi: https://doi.org/10.1042/BJ20131020
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