Signals derived from the BCR (B-cell antigen receptor) control survival, development and antigenic responses. One mechanism by which BCR signals may mediate these responses is by regulating cell metabolism. Indeed, the bioenergetic demands of naïve B-cells increase following BCR engagement and are characterized by a metabolic switch to aerobic glycolysis; however, the signalling pathways involved in this metabolic reprogramming are poorly defined. The PKC (protein kinase C) family plays an integral role in B-cell survival and antigenic responses. Using pharmacological inhibition and mice deficient in PKCβ, we demonstrate an essential role of PKCβ in BCR-induced glycolysis in B-cells. In contrast, mice deficient in PKCδ exhibit glycolytic rates comparable with those of wild-type B-cells following BCR cross-linking. The induction of several glycolytic genes following BCR engagement is impaired in PKCβ-deficient B-cells. Moreover, blocking glycolysis results in decreased survival of B-cells despite BCR engagement. The results establish a definitive role for PKCβ in the metabolic switch to glycolysis following BCR engagement of naïve B-cells.
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October 18 2012
Protein kinase Cβ is critical for the metabolic switch to glycolysis following B-cell antigen receptor engagement
Derek Blair;
Derek Blair
1
1Department of Biology, Boston College, 140 Commonwealth Avenue, 414 Higgins Hall, Chestnut Hill, MA 02467, U.S.A.
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Fay J. Dufort;
Fay J. Dufort
1Department of Biology, Boston College, 140 Commonwealth Avenue, 414 Higgins Hall, Chestnut Hill, MA 02467, U.S.A.
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Thomas C. Chiles
Thomas C. Chiles
2
1Department of Biology, Boston College, 140 Commonwealth Avenue, 414 Higgins Hall, Chestnut Hill, MA 02467, U.S.A.
2To whom correspondence should be addressed (email chilest@bc.edu).
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Publisher: Portland Press Ltd
Received:
August 01 2012
Revision Received:
September 04 2012
Accepted:
September 21 2012
Accepted Manuscript online:
September 21 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 448 (1): 165–169.
Article history
Received:
August 01 2012
Revision Received:
September 04 2012
Accepted:
September 21 2012
Accepted Manuscript online:
September 21 2012
Citation
Derek Blair, Fay J. Dufort, Thomas C. Chiles; Protein kinase Cβ is critical for the metabolic switch to glycolysis following B-cell antigen receptor engagement. Biochem J 15 November 2012; 448 (1): 165–169. doi: https://doi.org/10.1042/BJ20121225
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