DNA damage detection and repair take place in the context of chromatin, and histone proteins play important roles in these events. Post-translational modifications of histone proteins are involved in repair and DNA damage signalling processes in response to genotoxic stresses. In particular, acetylation of histones H3 and H4 plays an important role in the mammalian and yeast DNA damage response and survival under genotoxic stress. However, the role of post-translational modifications to histones during the plant DNA damage response is currently poorly understood. Several different acetylated H3 and H4 N-terminal peptides following X-ray treatment were identified using MS analysis of purified histones, revealing previously unseen patterns of histone acetylation in Arabidopsis. Immunoblot analysis revealed an increase in the relative abundance of the H3 acetylated N-terminus, and a global decrease in hyperacetylation of H4 in response to DNA damage induced by X-rays. Conversely, mutants in the key DNA damage signalling factor ATM (ATAXIA TELANGIECTASIA MUTATED) display increased histone acetylation upon irradiation, linking the DNA damage response with dynamic changes in histone modification in plants.
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August 2012
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Research Article|
July 13 2012
Dynamics of plant histone modifications in response to DNA damage
Georgina E. Drury;
Georgina E. Drury
*Centre for Plant Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, U.K.
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Adam A. Dowle;
Adam A. Dowle
†Proteomics Technology Facility, Department of Biology, University of York, York YO10 5DD, U.K.
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David A. Ashford;
David A. Ashford
†Proteomics Technology Facility, Department of Biology, University of York, York YO10 5DD, U.K.
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Wanda M. Waterworth;
Wanda M. Waterworth
*Centre for Plant Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, U.K.
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Jerry Thomas;
Jerry Thomas
†Proteomics Technology Facility, Department of Biology, University of York, York YO10 5DD, U.K.
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Christopher E. West
Christopher E. West
1
*Centre for Plant Sciences, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, U.K.
1To whom correspondence should be addressed (email c.e.west@leeds.ac.uk).
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Publisher: Portland Press Ltd
Received:
December 09 2011
Revision Received:
March 26 2012
Accepted:
May 11 2012
Accepted Manuscript online:
May 11 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 445 (3): 393–401.
Article history
Received:
December 09 2011
Revision Received:
March 26 2012
Accepted:
May 11 2012
Accepted Manuscript online:
May 11 2012
Citation
Georgina E. Drury, Adam A. Dowle, David A. Ashford, Wanda M. Waterworth, Jerry Thomas, Christopher E. West; Dynamics of plant histone modifications in response to DNA damage. Biochem J 1 August 2012; 445 (3): 393–401. doi: https://doi.org/10.1042/BJ20111956
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