The permeabilized cells and muscle fibres technique allows one to study the functional properties of mitochondria without their isolation, thus preserving all of the contacts with cellular structures, mostly the cytoskeleton, to study the whole mitochondrial population in the cell in their natural surroundings and it is increasingly being used in both experimental and clinical studies. The functional parameters (affinity for ADP in regulation of respiration) of mitochondria in permeabilized myocytes or myocardial fibres are very different from those in isolated mitochondria in vitro. In the present study, we have analysed the data showing the dependence of this parameter upon the muscle contractile state. Most remarkable is the effect of recently described Ca2+-independent contraction of permeabilized muscle fibres induced by elevated temperatures (30–37°C). We show that very similar strong spontaneous Ca2+-independent contraction can be produced by proteolytic treatment of permeabilized muscle fibres that result in a disorganization of mitochondrial arrangement, leading to a significant increase in affinity for ADP. These data show that Ca2+-insensitive contraction may be related to the destruction of cytoskeleton structures by intracellular proteases. Therefore the use of their inhibitors is strongly advised at the permeabilization step with careful washing of fibres or cells afterwards. A possible physiologically relevant relationship between Ca2+-regulated ATP-dependent contraction and mitochondrial functional parameters is also discussed.
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Research Article|
July 13 2012
Mysterious Ca2+-independent muscular contraction: déjà vu
Andrey V. Kuznetsov;
Andrey V. Kuznetsov
*Cardiac Surgery Research Laboratory, Department of Heart Surgery, Innsbruck Medical University, Innsbruck, Austria
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Rita Guzun;
Rita Guzun
†INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France
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François Boucher;
François Boucher
†INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France
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Rafaela Bagur;
Rafaela Bagur
†INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France
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Tuuli Kaambre;
Tuuli Kaambre
‡Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia
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Valdur Saks
Valdur Saks
1
†INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France
‡Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia
1To whom correspondence should be addressed (email Valdur.Saks@ujf-grenoble.fr).
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Publisher: Portland Press Ltd
Received:
March 12 2012
Revision Received:
May 02 2012
Accepted:
May 03 2012
Accepted Manuscript online:
May 03 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 445 (3): 333–336.
Article history
Received:
March 12 2012
Revision Received:
May 02 2012
Accepted:
May 03 2012
Accepted Manuscript online:
May 03 2012
Citation
Andrey V. Kuznetsov, Rita Guzun, François Boucher, Rafaela Bagur, Tuuli Kaambre, Valdur Saks; Mysterious Ca2+-independent muscular contraction: déjà vu. Biochem J 1 August 2012; 445 (3): 333–336. doi: https://doi.org/10.1042/BJ20120439
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