MAPK (mitogen-activated protein kinase) pathways have been implicated in stress signalling in plants. In the present study, we performed yeast two-hybrid screening to identify partner MAPKs for OsMKK (Oryza sativa MAPK kinase) 6, a rice MAPK kinase, and revealed specific interactions of OsMKK6 with OsMPK3 and OsMPK6. OsMPK3 and OsMPK6 each co-immunoprecipitated OsMKK6, and both were directly phosphorylated by OsMKK6 in vitro. An MBP (myelin basic protein) kinase assay of the immunoprecipitation complex indicated that OsMPK3 and OsMPK6 were activated in response to a moderately low temperature (12°C), but not a severely low temperature (4°C) in rice seedlings. A constitutively active form of OsMKK6, OsMKK6DD, showed elevated phosphorylation activity against OsMPK3 and OsMPK6 in vitro. OsMPK3, but not OsMPK6, was constitutively activated in transgenic plants overexpressing OsMKK6DD, indicating that OsMPK3 is an in vivo target of OsMKK6. Enhanced chilling tolerance was observed in the transgenic plants overexpressing OsMKK6DD. Taken together, our data suggest that OsMKK6 and OsMPK3 constitute a moderately low-temperature signalling pathway and regulate cold stress tolerance in rice.
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Research Article|
March 14 2012
Biochemical identification of the OsMKK6–OsMPK3 signalling pathway for chilling stress tolerance in rice1
Guosheng Xie;
Guosheng Xie
*Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan
†National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
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Hideki Kato;
Hideki Kato
*Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan
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Ryozo Imai
Ryozo Imai
2
*Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan
2To whom correspondence should be addressed (email rzi@affrc.go.jp).
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Publisher: Portland Press Ltd
Received:
October 06 2011
Revision Received:
December 12 2011
Accepted:
January 16 2012
Accepted Manuscript online:
January 16 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 443 (1): 95–102.
Article history
Received:
October 06 2011
Revision Received:
December 12 2011
Accepted:
January 16 2012
Accepted Manuscript online:
January 16 2012
Citation
Guosheng Xie, Hideki Kato, Ryozo Imai; Biochemical identification of the OsMKK6–OsMPK3 signalling pathway for chilling stress tolerance in rice. Biochem J 1 April 2012; 443 (1): 95–102. doi: https://doi.org/10.1042/BJ20111792
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