Transcriptional regulation of gene expression is one major determinant of developmental control and stress adaptation in virtually all living organisms. In recent years numerous transcription factors controlling various aspects of plant life have been identified. The activity of transcription factors needs to be regulated to prevent unspecific, prolonged or inappropriate responses. The transcription factor DREB2A (DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. In the present paper we describe evidence that the interaction with RCD1 (RADICAL-INDUCED CELL DEATH 1) contributes to the control of DREB2A under a range of conditions. The interaction is mediated by a novel protein motif in DREB2A and a splice variant of DREB2A which lacks the interaction domain accumulates during heat stress and senescence. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions.
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March 2012
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Research Article|
February 24 2012
RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana
Julia P. Vainonen;
Julia P. Vainonen
1
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Pinja Jaspers;
Pinja Jaspers
1
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Michael Wrzaczek;
Michael Wrzaczek
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Airi Lamminmäki;
Airi Lamminmäki
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Ramesha A. Reddy;
Ramesha A. Reddy
2
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Lauri Vaahtera;
Lauri Vaahtera
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
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Mikael Brosché;
Mikael Brosché
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
†Institute of Technology, University of Tartu, Nooruse 1, Tartu 50411, Estonia
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Jaakko Kangasjärvi
Jaakko Kangasjärvi
3
*Plant Biology, Department of Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland
3To whom correspondence should be addressed (jaakko.kangasjarvi@helsinki.fi).
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Publisher: Portland Press Ltd
Received:
September 28 2011
Revision Received:
December 05 2011
Accepted:
December 12 2011
Accepted Manuscript online:
December 12 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 442 (3): 573–581.
Article history
Received:
September 28 2011
Revision Received:
December 05 2011
Accepted:
December 12 2011
Accepted Manuscript online:
December 12 2011
Citation
Julia P. Vainonen, Pinja Jaspers, Michael Wrzaczek, Airi Lamminmäki, Ramesha A. Reddy, Lauri Vaahtera, Mikael Brosché, Jaakko Kangasjärvi; RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem J 15 March 2012; 442 (3): 573–581. doi: https://doi.org/10.1042/BJ20111739
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