The cytosolic GST (glutathione transferase) superfamily has been annotated in the Drosophila melanogaster genome database. Of 36 genes, four undergo alternative splicing to yield a total of 41 GST proteins. In the present study, we have obtained the 41 transcripts encoding proteins by RT (reverse transcription)–PCR using RNA template from Drosophila S2 cells, an embryonic cell line. This observation suggests that all of the annotated DmGSTs (D. melanogaster GSTs) in the proteome are expressed in the late embryonic stages of D. melanogaster. To avoid confusion in naming these numerous DmGSTs, we have designated them following the universal GST nomenclature as well as previous designations that fit within this classification. Furthermore, in the cell line, we identified an apparent processed pseudogene, gste8, in addition to two isoforms from the Delta class that have been published previously. Only approximately one-third of the expressed DmGSTs could be purified by conventional GSH affinity chromatography. The diverse kinetic properties as well as physiological substrate specificity of the DmGSTs are such that each individual enzyme displayed a unique character even compared with members from the same class.
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Research Article|
January 27 2012
A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster
Chonticha Saisawang;
Chonticha Saisawang
1Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom 73170, Thailand
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Jantana Wongsantichon;
Jantana Wongsantichon
1Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom 73170, Thailand
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Albert J. Ketterman
Albert J. Ketterman
1
1Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom 73170, Thailand
1To whom correspondence should be addressed (email albertketterman@yahoo.com).
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Publisher: Portland Press Ltd
Received:
September 29 2011
Revision Received:
November 14 2011
Accepted:
November 15 2011
Accepted Manuscript online:
November 15 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 442 (1): 181–190.
Article history
Received:
September 29 2011
Revision Received:
November 14 2011
Accepted:
November 15 2011
Accepted Manuscript online:
November 15 2011
Citation
Chonticha Saisawang, Jantana Wongsantichon, Albert J. Ketterman; A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster. Biochem J 15 February 2012; 442 (1): 181–190. doi: https://doi.org/10.1042/BJ20111747
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