The fungal toxin fusicoccin induces plant wilting by affecting ion transport across the plasma membrane of plant cell. The activity of this toxin is so far unknown in humans. In the present study we show that fusicoccin is able to affect the platelet aggregation process. The toxin associates with platelet intracellular binding sites and induces aggregation in platelet-rich plasma in a dose-dependent manner. We identified the adhesion receptor glycoprotein Ib-IX-V as fusicoccin target. The toxin promotes the binding of the regulatory 14-3-3 proteins to glycoprotein Ibα and hampers that to glycoprotein Ibβ subunit. As a result, platelet adhesion to von Willebrand factor is stimulated, leading to platelet spreading and integrin αIIbβ3 activation. We anticipate the present study to be a starting point for future therapeutic use of fusicoccin in genetic bleeding diseases characterized by qualitative or quantitative abnormalities of the platelet membrane-adhesion receptors. Furthermore, the present study also sets the stage for future work to determine the potential pharmacological application of fusicoccin as a drug directed to other 14-3-3–target complexes.
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Research Article|
May 13 2011
The phytotoxin fusicoccin promotes platelet aggregation via 14-3-3–glycoprotein Ib-IX-V interaction1
Lorenzo Camoni;
1Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy
3To whom correspondence should be addressed (email camoni@uniroma2.it).
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Cristina Di Lucente;
Cristina Di Lucente
2
1Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy
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Sabina Visconti;
Sabina Visconti
1Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy
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Patrizia Aducci
Patrizia Aducci
1Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy
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Publisher: Portland Press Ltd
Received:
December 06 2010
Revision Received:
March 11 2011
Accepted:
March 11 2011
Accepted Manuscript online:
March 11 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 436 (2): 429–436.
Article history
Received:
December 06 2010
Revision Received:
March 11 2011
Accepted:
March 11 2011
Accepted Manuscript online:
March 11 2011
Citation
Lorenzo Camoni, Cristina Di Lucente, Sabina Visconti, Patrizia Aducci; The phytotoxin fusicoccin promotes platelet aggregation via 14-3-3–glycoprotein Ib-IX-V interaction. Biochem J 1 June 2011; 436 (2): 429–436. doi: https://doi.org/10.1042/BJ20102037
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