Chromatin modifications play important roles in cellular biological process. A novel conserved domain family, YEATS, has been discovered in a variety of eukaryotic species ranging from yeasts to humans. Taf14, which is involved in a few protein complexes of chromatin remodelling and gene transcription, and is essential for keeping chromosome stability, regular cell growth and transcriptional regulation, contains a YEATS domain at its N-terminus. In the present study, we determined the solution structure of the Taf14 YEATS domain using NMR spectroscopy. The Taf14 YEATS domain adopts a global fold of an elongated β-sandwich, similar to the Yaf9 YEATS domain. However, the Taf14 YEATS domain differs significantly from the Yaf9 YEATS domain in some aspects, which might indicate different structural classes of the YEATS domain family. Functional studies indicate that the YEATS domain is critical for the function of Taf14 in inhibiting cell growth under stress conditions. In addition, our results show that the C-terminus of Taf14 is responsible for its interaction with Sth1, which is an essential component of the RSC complex. Taken together, this implies that Taf14 is involved in transcriptional activation of Saccharomyces cerevisiae and the YEATS domain of Taf14 might play a negative role in cell growth.
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Research Article|
April 27 2011
Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae
Wen Zhang;
Wen Zhang
*Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China
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Jiahai Zhang;
Jiahai Zhang
*Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China
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Xuecheng Zhang;
Xuecheng Zhang
†School of Life Sciences, Anhui University, Hefei, Anhui 230039, China
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Chao Xu;
Chao Xu
‡Structural Genomics Consortium, University of Toronto, 101 College St, Toronto, Ontario, Canada M5G 1L7
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Xiaoming Tu
Xiaoming Tu
1
*Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China
1To whom correspondence should be addressed (email xmtu@ustc.edu.cn).
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Publisher: Portland Press Ltd
Received:
January 04 2011
Revision Received:
February 18 2011
Accepted:
February 28 2011
Accepted Manuscript online:
February 28 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 436 (1): 83–90.
Article history
Received:
January 04 2011
Revision Received:
February 18 2011
Accepted:
February 28 2011
Accepted Manuscript online:
February 28 2011
Citation
Wen Zhang, Jiahai Zhang, Xuecheng Zhang, Chao Xu, Xiaoming Tu; Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae. Biochem J 15 May 2011; 436 (1): 83–90. doi: https://doi.org/10.1042/BJ20110004
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