The TRIM (tripartite motif) family of proteins is characterized by the presence of the tripartite motif module, composed of a RING domain, one or two B-box domains and a coiled-coil region. TRIM proteins are involved in many cellular processes and represent the largest subfamily of RING-containing putative ubiquitin E3 ligases. Whereas their role as E3 ubiquitin ligases has been presumed, and in several cases established, little is known about their specific interactions with the ubiquitin-conjugating E2 enzymes or UBE2s. In the present paper, we report a thorough screening of interactions between the TRIM and UBE2 families. We found a general preference of the TRIM proteins for the D and E classes of UBE2 enzymes, but we also revealed very specific interactions between TRIM9 and UBE2G2, and TRIM32 and UBE2V1/2. Furthermore, we demonstrated that the TRIM E3 activity is only manifest with the UBE2 with which they interact. For most specific interactions, we could also observe subcellular co-localization of the TRIM involved and its cognate UBE2 enzyme, suggesting that the specific selection of TRIM–UBE2 pairs has physiological relevance. Our findings represent the basis for future studies on the specific reactions catalysed by the TRIM E3 ligases to determine the fate of their targets.
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Research Article|
February 11 2011
Functional interactions between ubiquitin E2 enzymes and TRIM proteins
Luisa M. Napolitano;
Luisa M. Napolitano
*Cluster in Biomedicine (CBM), AREA Science Park, s.s. 14 km 163.5 Basovizza, 34149 Trieste, Italy
†Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131 Naples, Italy
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Ellis G. Jaffray;
Ellis G. Jaffray
‡Wellcome Trust Centre for Gene Regulation and Expression, College of Life Science, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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Ronald T. Hay;
Ronald T. Hay
‡Wellcome Trust Centre for Gene Regulation and Expression, College of Life Science, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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Germana Meroni
Germana Meroni
1
*Cluster in Biomedicine (CBM), AREA Science Park, s.s. 14 km 163.5 Basovizza, 34149 Trieste, Italy
1To whom correspondence should be addressed (email germana.meroni@cbm.fvg.it).
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Publisher: Portland Press Ltd
Received:
September 15 2010
Revision Received:
December 03 2010
Accepted:
December 08 2010
Accepted Manuscript online:
December 08 2010
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 434 (2): 309–319.
Article history
Received:
September 15 2010
Revision Received:
December 03 2010
Accepted:
December 08 2010
Accepted Manuscript online:
December 08 2010
Citation
Luisa M. Napolitano, Ellis G. Jaffray, Ronald T. Hay, Germana Meroni; Functional interactions between ubiquitin E2 enzymes and TRIM proteins. Biochem J 1 March 2011; 434 (2): 309–319. doi: https://doi.org/10.1042/BJ20101487
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