LDs (lipid droplets) are cellular organelles which can be found in nearly all eukaryotic cells. Despite their importance in cell biology, the mechanism underlying LD biogenesis remains largely unknown. In the present study we report that conditions of ER (endoplasmic reticulum) stress stimulate LD formation in Saccharomyces cerevisiae. We found that LDs accumulated in yeast mutants with compromised protein glycosylation or ER-associated protein degradation. Moreover, tunicamycin and Brefeldin A, agents which induce ER stress, were found to stimulate LD formation. In contrast, the restoration of protein glycosylation reduced LD accumulation. Interestingly, enhanced neutral lipids synthesis and LD formation under conditions of ER stress was not dependent on Ire1p. Lastly, we demonstrated that the absence of LDs did not compromise cell viability under ER stress. Our results suggest that although more LDs are produced, LDs are not essential to cell survival under ER stress.
Skip Nav Destination
Article navigation
November 2009
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
October 23 2009
Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae
Weihua Fei;
Weihua Fei
*School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
Search for other works by this author on:
Han Wang;
Han Wang
†Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore
Search for other works by this author on:
Xin Fu;
Xin Fu
†Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore
Search for other works by this author on:
Christopher Bielby;
Christopher Bielby
*School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
Search for other works by this author on:
Hongyuan Yang
Hongyuan Yang
1
*School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
†Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore
1To whom correspondence should be addressed (email h.rob.yang@unsw.edu.au).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
May 22 2009
Revision Received:
August 20 2009
Accepted:
August 26 2009
Accepted Manuscript online:
August 26 2009
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem J (2009) 424 (1): 61–67.
Article history
Received:
May 22 2009
Revision Received:
August 20 2009
Accepted:
August 26 2009
Accepted Manuscript online:
August 26 2009
Citation
Weihua Fei, Han Wang, Xin Fu, Christopher Bielby, Hongyuan Yang; Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae. Biochem J 15 November 2009; 424 (1): 61–67. doi: https://doi.org/10.1042/BJ20090785
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.