SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins contribute to specific recognition between transport vesicles and target membranes and are required for fusion of membranes. The SNARE Vti1p is required for several transport steps between late Golgi, endosomes and the vacuole in the yeast Saccharomyces cerevisiae. Here, we identified the late Golgi membrane protein TVP23 as a multicopy suppressor of the growth defect in vti1-2 cells. By contrast, the growth defect in vti1-11 cells was not suppressed by TVP23 overexpression. Deletion of TVP23 aggravated the growth defect in vti1-2 cells. Genetic interactions between TVP23 and vti1-2 were not found in transport from the late Golgi via the late endosome to the vacuole or in transport from the Golgi directly to the vacuole. These results suggest that Tvp23p is not involved in forward transport from the late Golgi. Therefore retrograde traffic to the late Golgi was analysed. vti1-2 cells accumulated GFP (green fluorescent protein)–Snc1p within the cell, indicating that retrograde transport from the early endosome to the late Golgi was defective in these cells. Deletion of TVP23 in vti1-2 cells resulted in a synthetic defect in GFP–Snc1p recycling, whereas tvp23Δ cells had a slight defect. These results indicate that Tvp23p performs a partially redundant function in retrograde transport from the early endosome to the late Golgi. This transport step was unaffected in vti1-11 cells, providing an explanation for the allele-specific multicopy suppression by TVP23.
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Research Article|
March 13 2009
TVP23 interacts genetically with the yeast SNARE VTI1 and functions in retrograde transport from the early endosome to the late Golgi
Ivar S. Stein;
Ivar S. Stein
1
1Biochemie III, Fakultät für Chemie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany
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Anna Gottfried;
Anna Gottfried
1
1Biochemie III, Fakultät für Chemie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany
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Jana Zimmermann;
Jana Zimmermann
1Biochemie III, Fakultät für Chemie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany
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Gabriele Fischer von Mollard
Gabriele Fischer von Mollard
2
1Biochemie III, Fakultät für Chemie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany
2To whom correspondence should be addressed (email gabriele.mollard@uni-bielefeld.de).
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Publisher: Portland Press Ltd
Received:
September 30 2008
Revision Received:
November 27 2008
Accepted:
December 15 2008
Accepted Manuscript online:
December 15 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem J (2009) 419 (1): 229–236.
Article history
Received:
September 30 2008
Revision Received:
November 27 2008
Accepted:
December 15 2008
Accepted Manuscript online:
December 15 2008
Citation
Ivar S. Stein, Anna Gottfried, Jana Zimmermann, Gabriele Fischer von Mollard; TVP23 interacts genetically with the yeast SNARE VTI1 and functions in retrograde transport from the early endosome to the late Golgi. Biochem J 1 April 2009; 419 (1): 229–236. doi: https://doi.org/10.1042/BJ20081973
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