GSTs (glutathione transferases) are an important class of enzymes involved in cellular detoxification. GSTs are found in all classes of organisms and are implicated in resistance towards drugs, pesticides, herbicides and antibiotics. The activity, structure and folding, particularly of eukaryotic GSTs, have therefore been widely studied. The crystal structure of EGST (GST from Escherichia coli) was reported around 10 years ago and it suggested Cys10 and His106 as potential catalytic residues. However, the role of these residues in catalysis has not been further investigated, nor have the folding properties of the protein been described. In the present study we investigated the contributions of residues Cys10 and His106 to the activity and stability of EGST. We found that EGST shows a complex equilibrium unfolding profile, involving a population of at least two partially folded intermediates, one of which is dimeric. Mutation of residues Cys10 and His106 leads to stabilization of the protein and affects the apparent steady-state kinetic parameters for enzyme catalysis. The results suggest that the imidazole ring of His106 plays an important role in the catalytic mechanism of the enzyme, whereas Cys10 is involved in binding of the substrate, glutathione. Engineering of the Cys10 site can be used to increase both the stability and GST activity of EGST. However, in addition to GST activity, we discovered that EGST also possesses thiol:disulfide oxidoreductase activity, for which the residue Cys10 plays an essential role. Further, tryptophan quenching experiments indicate that a mixed disulfide is formed between the free thiol group of Cys10 and the substrate, glutathione.
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Research Article|
December 12 2008
Characterization of the activity and folding of the glutathione transferase from Escherichia coli and the roles of residues Cys10 and His106
Xin-Yu Wang;
Xin-Yu Wang
*National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China
†Department of Biophysics, Institute of Physics, Nankai University, 94 Weijin Road, Tianjin 300071, People's Republic of China
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Zai-Rong Zhang;
Zai-Rong Zhang
*National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China
‡Graduate University of the Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan District, Beijing 100049, People's Republic of China
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Sarah Perrett
Sarah Perrett
1
*National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China
1To whom correspondence should be addressed (email sarah.perrett@iname.com).
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Publisher: Portland Press Ltd
Received:
December 19 2007
Revision Received:
August 19 2008
Accepted:
September 09 2008
Accepted Manuscript online:
September 09 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem J (2009) 417 (1): 55–64.
Article history
Received:
December 19 2007
Revision Received:
August 19 2008
Accepted:
September 09 2008
Accepted Manuscript online:
September 09 2008
Citation
Xin-Yu Wang, Zai-Rong Zhang, Sarah Perrett; Characterization of the activity and folding of the glutathione transferase from Escherichia coli and the roles of residues Cys10 and His106. Biochem J 1 January 2009; 417 (1): 55–64. doi: https://doi.org/10.1042/BJ20071702
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