The dual-specificity protein kinase Mps1 (monopolar spindle 1) is a phosphoprotein required for error-free mitotic progression in eukaryotes. In the present study, we have investigated human Mps1 phosphorylation using combined mass spectrometric, mutational and phosphospecific antibody approaches. We have identified 16 sites of Mps1 autophosphorylation in vitro, several of which are required for catalytic activity after expression in bacteria or in cultured human cells. Using novel phosphospecific antibodies, we show that endogenous Mps1 is phosphorylated on Thr686 and Ser821 during mitosis, and demonstrate that phosphorylated Mps1 localizes to the centrosomes of metaphase cells. Taken together, these results reveal the complexity of Mps1 regulation by multi-site phosphorylation, and demonstrate conclusively that phosphorylated Mps1 associates with centrosomes in mitotic human cells.
Skip Nav Destination
Article navigation
January 2009
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
December 12 2008
Phosphoregulation of human Mps1 kinase
Rebecca K. Tyler;
Rebecca K. Tyler
*Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, U.K.
Search for other works by this author on:
Matthew L. H. Chu;
Matthew L. H. Chu
†School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PT, U.K.
Search for other works by this author on:
Hannah Johnson;
Hannah Johnson
‡Michael Barber Centre for Mass Spectrometry, Manchester Interdisciplinary Biocentre, School of Chemistry, University of Manchester, Manchester M1 7DN, U.K.
Search for other works by this author on:
Edward A. McKenzie;
Edward A. McKenzie
*Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, U.K.
Search for other works by this author on:
Simon J. Gaskell;
Simon J. Gaskell
‡Michael Barber Centre for Mass Spectrometry, Manchester Interdisciplinary Biocentre, School of Chemistry, University of Manchester, Manchester M1 7DN, U.K.
Search for other works by this author on:
Patrick A. Eyers
Patrick A. Eyers
1
*Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, U.K.
1To whom correspondence should be addressed at the present address: Yorkshire Cancer Research (YCR) Institute for Cancer Studies, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2RY, U.K. (email p.eyers@sheffield.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 27 2008
Revision Received:
July 31 2008
Accepted:
August 05 2008
Accepted Manuscript online:
August 05 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem J (2009) 417 (1): 173–184.
Article history
Received:
June 27 2008
Revision Received:
July 31 2008
Accepted:
August 05 2008
Accepted Manuscript online:
August 05 2008
Citation
Rebecca K. Tyler, Matthew L. H. Chu, Hannah Johnson, Edward A. McKenzie, Simon J. Gaskell, Patrick A. Eyers; Phosphoregulation of human Mps1 kinase. Biochem J 1 January 2009; 417 (1): 173–184. doi: https://doi.org/10.1042/BJ20081310
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.