MUC5B is the predominant polymeric mucin in human saliva [Thornton, Khan, Mehrotra, Howard, Veerman, Packer and Sheehan (1999) Glycobiology 9, 293–302], where it contributes to oral cavity hydration and protection. More recently, the gene for another putative polymeric mucin, MUC19, has been shown to be expressed in human salivary glands [Chen, Zhao, Kalaslavadi, Hamati, Nehrke, Le, Ann and Wu (2004) Am. J. Respir. Cell Mol. Biol. 30, 155–165]. However, to date, the MUC19 mucin has not been isolated from human saliva. Our aim was therefore to purify and characterize the MUC19 glycoprotein from human saliva. Saliva was solubilized in 4 M guanidinium chloride and the high-density mucins were purified by density-gradient centrifugation. The presence of MUC19 was investigated using tandem MS of tryptic peptides derived from this mucin preparation. Using this approach, we found multiple MUC5B-derived tryptic peptides, but were unable to detect any putative MUC19 peptides. These results suggest that MUC19 is not a major component in human saliva. In contrast, using the same experimental approach, we identified Muc19 and Muc5b glycoproteins in horse saliva. Moreover, we also identified Muc19 from pig, cow and rat saliva; the saliva of cow and rat also contained Muc5b; however, due to the lack of pig Muc5b genomic sequence data, we were unable to identify Muc5b in pig saliva. Our results suggest that unlike human saliva, which contains MUC5B, cow, horse and rat saliva are a heterogeneous mixture of Muc5b and Muc19. The functional consequence of these species differences remains to be elucidated.
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Research Article|
July 15 2008
Proteomic analysis of polymeric salivary mucins: no evidence for MUC19 in human saliva
Karine Rousseau;
Karine Rousseau
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Sara Kirkham;
Sara Kirkham
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Lindsay Johnson;
Lindsay Johnson
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Brian Fitzpatrick;
Brian Fitzpatrick
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Marj Howard;
Marj Howard
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Emily J. Adams;
Emily J. Adams
†Airway Disease, National Heart and Lung Institute, Imperial College London, London SW3 6LY, U.K.
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Duncan F. Rogers;
Duncan F. Rogers
†Airway Disease, National Heart and Lung Institute, Imperial College London, London SW3 6LY, U.K.
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David Knight;
David Knight
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
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Peter Clegg;
Peter Clegg
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
‡Faculty of Veterinary Science, University of Liverpool, Leahurst, Neston CH64 7TE, U.K.
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David J. Thornton
David J. Thornton
1
*Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, U.K.
1To whom correspondence should be addressed (email dave.thornton@manchester.ac.uk).
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Publisher: Portland Press Ltd
Received:
January 30 2008
Revision Received:
April 21 2008
Accepted:
April 22 2008
Accepted Manuscript online:
April 22 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 413 (3): 545–552.
Article history
Received:
January 30 2008
Revision Received:
April 21 2008
Accepted:
April 22 2008
Accepted Manuscript online:
April 22 2008
Citation
Karine Rousseau, Sara Kirkham, Lindsay Johnson, Brian Fitzpatrick, Marj Howard, Emily J. Adams, Duncan F. Rogers, David Knight, Peter Clegg, David J. Thornton; Proteomic analysis of polymeric salivary mucins: no evidence for MUC19 in human saliva. Biochem J 1 August 2008; 413 (3): 545–552. doi: https://doi.org/10.1042/BJ20080260
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