Sec (selenocysteine) is biosynthesized on its tRNA and incorporated into selenium-containing proteins (selenoproteins) as the 21st amino acid residue. Selenoprotein synthesis is dependent on Sec tRNA and the expression of this class of proteins can be modulated by altering Sec tRNA expression. The gene encoding Sec tRNA (Trsp) is a single-copy gene and its targeted removal in liver demonstrated that selenoproteins are essential for proper function wherein their absence leads to necrosis and hepatocellular degeneration. In the present study, we found that the complete loss of selenoproteins in liver was compensated for by an enhanced expression of several phase II response genes and their corresponding gene products. The replacement of selenoprotein synthesis in mice carrying mutant Trsp transgenes, wherein housekeeping, but not stress-related selenoproteins are expressed, led to normal expression of phase II response genes. Thus the present study provides evidence for a functional link between housekeeping selenoproteins and phase II enzymes.
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Research Article|
June 12 2008
A functional link between housekeeping selenoproteins and phase II enzymes
Aniruddha Sengupta;
Aniruddha Sengupta
*Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, CCR, NCI, NIH, Bethesda, MD 20892, U.S.A.
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Bradley A. Carlson;
Bradley A. Carlson
*Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, CCR, NCI, NIH, Bethesda, MD 20892, U.S.A.
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James A. Weaver;
James A. Weaver
*Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, CCR, NCI, NIH, Bethesda, MD 20892, U.S.A.
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Sergey V. Novoselov;
Sergey V. Novoselov
†Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, U.S.A.
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Dmitri E. Fomenko;
Dmitri E. Fomenko
†Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, U.S.A.
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Vadim N. Gladyshev;
Vadim N. Gladyshev
†Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, U.S.A.
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Dolph L. Hatfield
Dolph L. Hatfield
1
*Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, CCR, NCI, NIH, Bethesda, MD 20892, U.S.A.
1To whom correspondence should be addressed (email hatfield@mail.nih.gov).
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Publisher: Portland Press Ltd
Received:
January 30 2008
Revision Received:
March 13 2008
Accepted:
March 28 2008
Accepted Manuscript online:
March 28 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 413 (1): 151–161.
Article history
Received:
January 30 2008
Revision Received:
March 13 2008
Accepted:
March 28 2008
Accepted Manuscript online:
March 28 2008
Citation
Aniruddha Sengupta, Bradley A. Carlson, James A. Weaver, Sergey V. Novoselov, Dmitri E. Fomenko, Vadim N. Gladyshev, Dolph L. Hatfield; A functional link between housekeeping selenoproteins and phase II enzymes. Biochem J 1 July 2008; 413 (1): 151–161. doi: https://doi.org/10.1042/BJ20080277
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