In a previous study, alcS, a gene of the Aspergillus nidulans alc cluster, was shown to encode a protein that belongs to the GPR1/FUN34/YaaH membrane protein family. BLAST screening of the A. nidulans genome data identified additional genes encoding hypothetical proteins that could belong to this family. In this study we report the functional characterization of one of them, AN5226. Its expression is induced by ethanol and ethyl acetate (two inducers of the alc genes) and is mediated by the specific transcriptional activator of genes of the acetate-utilization pathway FacB. Growth of a null mutant (ΔAN5226) is notably affected when acetate is used as sole carbon source at low concentration and in a high pH medium, i.e. when protonated acetate, the form that can enter the cell by passive diffusion, is present in low amounts. Consistently, expression of AN5226 is also induced by acetate, but only when the latter is present at low concentrations. 14C-labelled acetate uptake experiments using germinating conidia demonstrate an essential role for AN5226 in mediated acetate transport. To our knowledge this report is the first to provide evidence for the identification of an acetate transporter in filamentous fungi. We have designated AN5226 as acpA (for acetate permease A).
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Research Article|
May 28 2008
AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans
Xavier Robellet;
Xavier Robellet
*Institut de Génétique et Microbiologie, Unité Mixte de Recherche 8621, CNRS - Université Paris-Sud XI, Centre Scientifique d'Orsay, Bâtiment 360, F-91405 Orsay Cedex, France
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Michel Flipphi;
Michel Flipphi
†Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, 46100 Burjassot, Valencia, Spain
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Sylvine Pégot;
Sylvine Pégot
*Institut de Génétique et Microbiologie, Unité Mixte de Recherche 8621, CNRS - Université Paris-Sud XI, Centre Scientifique d'Orsay, Bâtiment 360, F-91405 Orsay Cedex, France
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Andrew P. MacCabe;
Andrew P. MacCabe
†Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, 46100 Burjassot, Valencia, Spain
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Christian Vélot
Christian Vélot
1
*Institut de Génétique et Microbiologie, Unité Mixte de Recherche 8621, CNRS - Université Paris-Sud XI, Centre Scientifique d'Orsay, Bâtiment 360, F-91405 Orsay Cedex, France
1To whom correspondence should be addressed (email christian.velot@igmors.u-psud.fr).
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Publisher: Portland Press Ltd
Received:
January 14 2008
Revision Received:
February 19 2008
Accepted:
February 27 2008
Accepted Manuscript online:
February 27 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 412 (3): 485–493.
Article history
Received:
January 14 2008
Revision Received:
February 19 2008
Accepted:
February 27 2008
Accepted Manuscript online:
February 27 2008
Citation
Xavier Robellet, Michel Flipphi, Sylvine Pégot, Andrew P. MacCabe, Christian Vélot; AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans. Biochem J 15 June 2008; 412 (3): 485–493. doi: https://doi.org/10.1042/BJ20080124
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