SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins involved in membrane fusion usually contain a conserved α-helix (SNARE motif) that is flanked by a C-terminal transmembrane domain. They can be classified into Q-SNARE and R-SNARE based on the structural property of their motifs. Assembly of four SNARE motifs (Qa, b, c and R) is supposed to trigger membrane fusion. We have previously shown that ER (endoplasmic reticulum)-localized syntaxin 18 (Qa) forms a complex with BNIP1 (Qb), p31/Use1 (Qc), Sec22b (R) and several peripheral membrane proteins. In the present study, we examined the interaction of syntaxin 18 with other SNAREs using pulldown assays and CD spectroscopy. We found that the association of syntaxin 18 with Sec22b induces an increase in α-helicity of their SNARE motifs, which results in the formation of high-affinity binding sites for BNIP1 and p31. This R-SNARE-dependent Q-SNARE assembly is quite different from the assembly mechanisms of SNAREs localized in organelles other than the ER. The implication of the mechanism of ER SNARE assembly is discussed in the context of the physiological roles of the syntaxin 18 complex.
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Research Article|
January 29 2008
Sec22b-dependent assembly of endoplasmic reticulum Q-SNARE proteins
Takehiro Aoki;
Takehiro Aoki
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan
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Masaki Kojima;
Masaki Kojima
1
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan
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Katsuko Tani;
Katsuko Tani
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan
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Mitsuo Tagaya
Mitsuo Tagaya
2
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan
2To whom correspondence should be addressed (e-mail tagaya@ls.toyaku.ac.jp).
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Publisher: Portland Press Ltd
Received:
September 21 2007
Revision Received:
October 31 2007
Accepted:
November 02 2007
Accepted Manuscript online:
November 02 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 410 (1): 93–100.
Article history
Received:
September 21 2007
Revision Received:
October 31 2007
Accepted:
November 02 2007
Accepted Manuscript online:
November 02 2007
Citation
Takehiro Aoki, Masaki Kojima, Katsuko Tani, Mitsuo Tagaya; Sec22b-dependent assembly of endoplasmic reticulum Q-SNARE proteins. Biochem J 15 February 2008; 410 (1): 93–100. doi: https://doi.org/10.1042/BJ20071304
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