YGL196W of Saccharomyces cerevisiae encodes a putative protein that is unidentified but is predicted to have a motif similar to that of the N-terminal domain of the bacterial alanine racemase. In the present study we found that YGL196W encodes a novel D-serine dehydratase, which belongs to a different protein family from that of the known bacterial enzyme. The yeast D-serine dehydratase purified from recombinant Escherichia coli cells depends on pyridoxal 5′-phosphate and zinc, and catalyses the conversion of D-serine into pyruvate and ammonia with the Km and kcat values of 0.39 mM and 13.1 s−1 respectively. D-Threonine and β-Cl-D-alanine also serve as substrates with catalytic efficiencies which are approx. 3 and 2% of D-serine respectively. L-Serine, L-threonine and β-Cl-L-alanine are inert as substrates. Atomic absorption analysis revealed that the enzyme contains one zinc atom per enzyme monomer. The enzyme activities toward D-serine and D-threonine were decreased by EDTA treatment and recovered by the addition of Zn2+. Little recovery was observed with Mg2+, Mn2+, Ca2+, Ni2+, Cu2+, K+ or Na+. In contrast, the activity towards β-Cl-D-alanine was retained after EDTA treatment. These results suggest that zinc is involved in the elimination of the hydroxy group of D-serine and D-threonine. D-Serine dehydratase of S. cerevisiae is probably the first example of a eukaryotic D-serine dehydratase and that of a specifically zinc-dependent pyridoxal enzyme as well.
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Research Article|
December 21 2007
A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae
Tomokazu Ito;
Tomokazu Ito
*Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Aichi, Japan
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Hisashi Hemmi;
Hisashi Hemmi
*Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Aichi, Japan
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Kunishige Kataoka;
Kunishige Kataoka
†Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Ishikawa, Japan
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Yukio Mukai;
Yukio Mukai
‡Nagahama Institute of Bio-Science and Technology, Nagahama 526-0829, Shiga, Japan
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Tohru Yoshimura
Tohru Yoshimura
1
*Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Aichi, Japan
1To whom correspondence should be addressed (email yosimura@agr.nagoya-u.ac.jp).
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Publisher: Portland Press Ltd
Received:
May 15 2007
Revision Received:
October 11 2007
Accepted:
October 16 2007
Accepted Manuscript online:
October 16 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 409 (2): 399–406.
Article history
Received:
May 15 2007
Revision Received:
October 11 2007
Accepted:
October 16 2007
Accepted Manuscript online:
October 16 2007
Citation
Tomokazu Ito, Hisashi Hemmi, Kunishige Kataoka, Yukio Mukai, Tohru Yoshimura; A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae. Biochem J 15 January 2008; 409 (2): 399–406. doi: https://doi.org/10.1042/BJ20070642
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