FgFtr1 and FgFtr2 are putative iron permeases, and FgFet1 and FgFet2 are putative ferroxidases of Fusarium graminearum. They have high homologies with iron permease ScFtr1 and ferroxidase ScFet3 of Saccharomyces cerevisiae at the amino acid level. The genes encoding iron permease and ferroxidase were localized to the same chromosome in the manner of FgFtr1/FgFet1 and FgFtr2/FgFet2. The GFP (green fluorescent protein)-fused versions of FgFtr1 and FgFtr2 showed normal functions when compared with FgFtr1 and FgFtr2 in an S. cerevisiae system, and the cellular localizations of FgFtr1 and FgFtr2 in S. cerevisiae depended on the expression of their putative ferroxidase partners FgFet1 and FgFet2 respectively. Although FgFtr1 was found on the plasma membrane when FgFet1 and FgFtr1 were co-transformed in S. cerevisiae, most of the FgFtr1 was found in the endoplasmic reticulum compartment when co-expressed with FgFet2. Furthermore, FgFtr2 was found on the vacuolar membrane when FgFet2 was co-expressed. From the two-hybrid analysis, we confirmed the interaction of FgFtr1 and FgFet1, and the same result was found between FgFtr2 and FgFet2. Iron-uptake activity also depended on the existence of the respective partner. Finally, the FgFtr1 and FgFtr2 were found on the plasma and vacuolar membrane respectively, in F. graminearum. Taken together, these results strongly suggest that FgFtr1 and FgFtr2 from F. graminearum encode the iron permeases of the plasma membrane and vacuolar membrane respectively, and require their specific ferroxidases to carry out normal function. Furthermore, the present study suggests that the reductive iron-uptake system is conserved from yeast to filamentous fungi.
Skip Nav Destination
Article navigation
November 2007
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
October 29 2007
Physical and functional interaction of FgFtr1–FgFet1 and FgFtr2–FgFet2 is required for iron uptake in Fusarium graminearum
Yong-Sung Park;
Yong-Sung Park
1
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Ji-Hyun Kim;
Ji-Hyun Kim
1
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Jin-Hwa Cho;
Jin-Hwa Cho
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Hyo-Ihl Chang;
Hyo-Ihl Chang
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Seung-Wook Kim;
Seung-Wook Kim
†Department of Chemical and Biological Engineering, Korea University. Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Hyun-Dong Paik;
Hyun-Dong Paik
‡Division of Animal Life Science, Konkuk University, Seoul, 143-701, Korea
Search for other works by this author on:
Chang-Won Kang;
Chang-Won Kang
‡Division of Animal Life Science, Konkuk University, Seoul, 143-701, Korea
Search for other works by this author on:
Tae-Hyoung Kim;
Tae-Hyoung Kim
§Chosun University School of Medicine, Department of Biochemistry and Molecular Biology, 375 Seosuk-Dong, Dong-gu, Gwangju, 501-759, Korea
Search for other works by this author on:
Ha-Chin Sung;
Ha-Chin Sung
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
Search for other works by this author on:
Cheol-Won Yun
Cheol-Won Yun
2
*School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, 143-701, Korea
2To whom correspondence should be addressed (email cheolwony@korea.ac.kr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 02 2007
Revision Received:
July 20 2007
Accepted:
July 27 2007
Accepted Manuscript online:
July 27 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem J (2007) 408 (1): 97–104.
Article history
Received:
April 02 2007
Revision Received:
July 20 2007
Accepted:
July 27 2007
Accepted Manuscript online:
July 27 2007
Citation
Yong-Sung Park, Ji-Hyun Kim, Jin-Hwa Cho, Hyo-Ihl Chang, Seung-Wook Kim, Hyun-Dong Paik, Chang-Won Kang, Tae-Hyoung Kim, Ha-Chin Sung, Cheol-Won Yun; Physical and functional interaction of FgFtr1–FgFet1 and FgFtr2–FgFet2 is required for iron uptake in Fusarium graminearum. Biochem J 15 November 2007; 408 (1): 97–104. doi: https://doi.org/10.1042/BJ20070450
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.