Although PTEN (phosphatase and tensin homologue deleted on chromosome 10) is one of the most commonly mutated tumour suppressors in human cancers, loss of PTEN expression in the absence of mutation appears to occur in an even greater number of tumours. PTEN is phosphorylated in vitro on Thr366 and Ser370 by GSK3 (glycogen synthase kinase 3) and CK2 (casein kinase 2) respectively, and specific inhibitors of these kinases block these phosphorylation events in cultured cells. Although mutation of these phosphorylation sites did not alter the phosphatase activity of PTEN in vitro or in cells, blocking phosphorylation of Thr366 by either mutation or GSK3 inhibition in glioblastoma cell lines led to a stabilization of the PTEN protein. Our data support a model in which the phosphorylation of Thr366 plays a role in destabilizing the PTEN protein.
Skip Nav Destination
Article navigation
August 2007
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
July 13 2007
PTEN is destabilized by phosphorylation on Thr366
Helene Maccario;
Helene Maccario
1
1Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
Search for other works by this author on:
Nevin M. Perera;
Nevin M. Perera
1
1Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
Search for other works by this author on:
Lindsay Davidson;
Lindsay Davidson
1Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
Search for other works by this author on:
C. Peter Downes;
C. Peter Downes
1Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
Search for other works by this author on:
Nick R. Leslie
Nick R. Leslie
2
1Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
2To whom correspondence should be addressed (email n.r.leslie@dundee.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
December 11 2006
Revision Received:
April 10 2007
Accepted:
April 20 2007
Accepted Manuscript online:
April 20 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2007 Biochemical Society
2007
Biochem J (2007) 405 (3): 439–444.
Article history
Received:
December 11 2006
Revision Received:
April 10 2007
Accepted:
April 20 2007
Accepted Manuscript online:
April 20 2007
Citation
Helene Maccario, Nevin M. Perera, Lindsay Davidson, C. Peter Downes, Nick R. Leslie; PTEN is destabilized by phosphorylation on Thr366. Biochem J 1 August 2007; 405 (3): 439–444. doi: https://doi.org/10.1042/BJ20061837
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.