Amyloid β-peptide (Aβ) is a major component of plaques in Alzheimer's disease, and formation of senile plaques has been suggested to originate from regions of neuronal membrane rich in gangliosides. Here we demonstrate using NMR on 15N-labelled Aβ-(1–40) and Aβ-(1–42) that the interaction with ganglioside GM1 micelles is localized to the N-terminal region of the peptide, particularly residues His13 to Leu17, which become more helical when bound. The key interaction is with His13, which undergoes a GM1-specific conformational change. The sialic acid residue of the ganglioside headgroup is important for determining the nature of the conformational change. The isolated pentasaccharide headgroup of GM1 is not bound, suggesting the need for a polyanionic surface. Binding to heparin confirms this suggestion, since binding is of similar affinity but does not produce the same conformational changes in the peptide. A comparison of Aβ-(1–40) and Aβ-(1–42) indicates that binding to GM1 micelles is not related to oligomerization, which occurs at the C-terminal end. These results imply that binding to ganglioside micelles causes a transition from random coil to α-helix in the N-terminal region, leaving the C-terminal region unstructured.
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Research Article|
July 13 2006
Binding of amyloid β-peptide to ganglioside micelles is dependent on histidine-13
Mike P. Williamson;
Mike P. Williamson
1
*Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, U.K.
1To whom correspondence should be addressed (email m.williamson@sheffield.ac.uk).
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Yu Suzuki;
Yu Suzuki
2
†Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
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Nathan T. Bourne;
Nathan T. Bourne
2
*Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, U.K.
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Tetsuo Asakura
Tetsuo Asakura
†Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
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Publisher: Portland Press Ltd
Received:
February 20 2006
Revision Received:
March 30 2006
Accepted:
April 21 2006
Accepted Manuscript online:
April 21 2006
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2006
Biochem J (2006) 397 (3): 483–490.
Article history
Received:
February 20 2006
Revision Received:
March 30 2006
Accepted:
April 21 2006
Accepted Manuscript online:
April 21 2006
Citation
Mike P. Williamson, Yu Suzuki, Nathan T. Bourne, Tetsuo Asakura; Binding of amyloid β-peptide to ganglioside micelles is dependent on histidine-13. Biochem J 1 August 2006; 397 (3): 483–490. doi: https://doi.org/10.1042/BJ20060293
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