The PABP [poly(A)-binding protein] is able to interact with the 3′ poly(A) tail of eukaryotic mRNA, promoting its translation. Cleavage of PABP by viral proteases encoded by several picornaviruses and caliciviruses plays a role in the abrogation of cellular protein synthesis. We report that infection of MT-2 cells with HIV-1 leads to efficient proteolysis of PABP. Analysis of PABP integrity was carried out in BHK-21 (baby-hamster kidney) and COS-7 cells upon individual expression of the protease from several members of the Retroviridae family, e.g. MoMLV (Moloney murine leukaemia virus), MMTV (mouse mammary tumour virus), HTLV-I (human T-cell leukaemia virus type I), SIV (simian immunodeficiency virus), HIV-1 and HIV-2. Moreover, protease activity against PABP was tested in a HeLa-cell-free system. Only MMTV, HIV-1 and HIV-2 proteases were able to cleave PABP in the absence of other viral proteins. Purified HIV-1 and HIV-2 proteases cleave PABP1 directly at positions 237 and 477, separating the two first RNA-recognition motifs from the C-terminal domain of PABP. An additional cleavage site located at position 410 was detected for HIV-2 protease. These findings indicate that some retroviruses may share with picornaviruses and caliciviruses the capacity to proteolyse PABP.
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Research Article|
May 15 2006
HIV protease cleaves poly(A)-binding protein
Enrique Álvarez;
Enrique Álvarez
1
1Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
1To whom correspondence should be addressed at the present address: Centro Nacional de Biotecnología (CSIC), Cantoblanco, 28049 Madrid, Spain (email ealvarez@cnb.uam.es).
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Alfredo Castelló;
Alfredo Castelló
1Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
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Luis Menéndez-Arias;
Luis Menéndez-Arias
1Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
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Luis Carrasco
Luis Carrasco
1Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
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Publisher: Portland Press Ltd
Received:
January 16 2006
Revision Received:
March 01 2006
Accepted:
March 02 2006
Accepted Manuscript online:
March 02 2006
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2006
Biochem J (2006) 396 (2): 219–226.
Article history
Received:
January 16 2006
Revision Received:
March 01 2006
Accepted:
March 02 2006
Accepted Manuscript online:
March 02 2006
Connected Content
A commentary has been published:
Cleavage, a real turn-off? HIV-mediated proteolysis of PABP1
Citation
Enrique Álvarez, Alfredo Castelló, Luis Menéndez-Arias, Luis Carrasco; HIV protease cleaves poly(A)-binding protein. Biochem J 1 June 2006; 396 (2): 219–226. doi: https://doi.org/10.1042/BJ20060108
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