HSF1 (heat-shock factor 1) plays an essential role in mediating the appropriate cellular response to diverse forms of physiological stresses. However, it is not clear how HSF1 is regulated by interacting proteins under normal and stressful conditions. In the present study, Hsc70 (heat-shock cognate 70) was identified as a HSF1-interacting protein using the TAP (tandem affinity purification) system and MS. HSF1 can interact with Hsc70 in vivo and directly in vitro. Interestingly, Hsc70 is required for the regulation of HSF1 during heat stress and subsequent target gene expression in mammalian cells. Moreover, cells transfected with siRNAs (small interfering RNAs) targeted to Hsc70 showed greatly decreased HSF1 activation with expression of HSF1 target genes being dramatically reduced. Finally, loss of Hsc70 expression in cells resulted in an increase in stress-induced apoptosis. These results indicate that Hsc70 is a necessary and critical regulator of HSF1 activities.
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Research Article|
November 08 2005
Heat-shock cognate 70 is required for the activation of heat-shock factor 1 in mammalian cells
Sang-Gun Ahn;
Sang-Gun Ahn
1
*Department of Pathology, Chosun University College of Dentistry, Gwangju 501-759, South Korea
1Correspondence can be addressed to either of these authors (email ahnsg@chosun.ac.kr or vacratsi@uwindsor.ca).
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Soo-A Kim;
Soo-A Kim
*Department of Pathology, Chosun University College of Dentistry, Gwangju 501-759, South Korea
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Jung-Hoon Yoon;
Jung-Hoon Yoon
*Department of Pathology, Chosun University College of Dentistry, Gwangju 501-759, South Korea
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Panayiotis Vacratsis
Panayiotis Vacratsis
1
†Department of Chemistry/Biochemistry, University of Windsor, Windsor, Ontario, Canada N9B 3P4
1Correspondence can be addressed to either of these authors (email ahnsg@chosun.ac.kr or vacratsi@uwindsor.ca).
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Publisher: Portland Press Ltd
Received:
March 11 2005
Revision Received:
July 12 2005
Accepted:
July 29 2005
Accepted Manuscript online:
July 29 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 392 (1): 145–152.
Article history
Received:
March 11 2005
Revision Received:
July 12 2005
Accepted:
July 29 2005
Accepted Manuscript online:
July 29 2005
Citation
Sang-Gun Ahn, Soo-A Kim, Jung-Hoon Yoon, Panayiotis Vacratsis; Heat-shock cognate 70 is required for the activation of heat-shock factor 1 in mammalian cells. Biochem J 15 November 2005; 392 (1): 145–152. doi: https://doi.org/10.1042/BJ20050412
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